Preparing cDNA Libraries from Lytic Phage-Infected Cells for Whole Transcriptome Analysis by RNA-Seq.

Preparing cDNA Libraries from Lytic Phage-Infected Cells for Whole Transcriptome Analysis by RNA-Seq.

Entire genome large evaluation of transcription utilizing RNA-Seq strategies is a strong strategy to elucidate differential expression of gene options in micro organism throughout completely different circumstances in addition to for locating beforehand unique RNA species.

Certainly, RNA sequencing has revolutionized the examine of bacterial transcription with the range and amount of small noncoding RNA parts which were discovered and its capability to obviously outline operons, promoters , and terminators . We focus on our expertise with making use of RNA sequencing know-how to analyzing the lytic cycle, together with extraction, processing, and a information to the custom-made statistical evaluation needed for analyzing differential host and phage transcription.

Speedy Development of Advanced Plant RNA Virus Infectious cDNA Clones for Agroinfection Utilizing a Yeast-E. coli-Agrobacterium Shuttle Vector.

The provision of infectious full-length clone is indispensable for reverse genetics research of virus biology, pathology and development of viral vectors. Nevertheless, for RNA viruses with giant genome sizes or these exhibiting inherent cloning difficulties, process to generate biologically lively round DNA (cDNA) clones will be time-consuming or technically difficult. Right here we have now constructed a yeast-Escherichia coli-Agrobacterium shuttle vector that permits extremely environment friendly homologous recombination in yeast for meeting of Agrobacterium suitable plant virus clones. Utilizing this vector, we present that infectious cDNA clones of a plant negative-stranded RNA virus, sonchus yellow web rhabdovirus, will be quickly assembled. As well as, one-step meeting of infectious clones of potato virus Y in yeast, both with or with out intron, was readily achieved from as many as eight overlapping DNA fragments.

Extra importantly, the recovered yeast plasmids will be remodeled immediately into Agrobacterium for inoculation, thereby obviating the E. coli cloning steps and related toxicity points. This technique is speedy, extremely environment friendly and cost-effective and needs to be readily relevant to a broad vary of plant viruses.

3′ RNA ligase mediated speedy amplification of cDNA ends for validating viroid induced cleavage on the 3′ extremity of the host mRNA.

5′ RNA ligase-mediated speedy amplification of cDNA ends (5′ RLM-RACE) is a widely-accepted technique for the validation of direct cleavage of a goal gene by a microRNA (miRNA) and viroid-derived small RNA (vd-sRNA). Nevertheless, this technique can’t be used if cleavage takes place within the 3′ extremity of the goal RNA, as this offers inadequate sequence size to design nested PCR primers for five’ RLM RACE. To beat this hurdle, we have now developed 3′ RNA ligase-mediated speedy amplification of cDNA ends (3′ RLM RACE).

On this technique, an oligonucleotide adapter having 5′ adenylated and three’ blocked is ligated to the three’ finish of the cleaved RNA adopted by PCR amplification utilizing gene particular primers. In different phrases, in 3′ RLM RACE, 3′ finish is mapped utilizing 5′ fragment as a substitute of small 3′ fragment.

Preparing cDNA Libraries from Lytic Phage-Infected Cells for Whole Transcriptome Analysis by RNA-Seq.

The strategy developed right here was verified by inspecting the bioinformatics predicted and parallel evaluation of RNA ends (PARE) proved cleavage websites of chloride channel protein CLC-b-like mRNA in Potato spindle tuber viroid contaminated tomato vegetation. The three’ RLM RACE developed on this examine has the potential to validate the miRNA and vd-sRNA mediated cleavage of mRNAs at its 3′ untranslated area (3′ UTR).

Stimulation of reverse transcriptase generated cDNAs with particular indels by template RNA construction: retrotransposon, dNTP steadiness, RT-reagent utilization.

RNA dependent DNA-polymerases, reverse transcriptases, are key enzymes for retroviruses and retroelements. Their constancy, together with indel technology, is important for his or her use as reagents together with for deep sequencing. Right here, we report that sure RNA template buildings and G-rich sequences, forward of numerous reverse transcriptases will be sturdy stimulators for slippage at slippage-prone template motif sequence 3′ of such ‘slippage-stimulatory’ buildings. The place slippage is stimulated, the ensuing merchandise have a number of extra base(s) in comparison with the corresponding template motif.

Such buildings additionally inhibit slippage-mediated base omission which will be extra frequent within the absence of a related stem-loop. Slippage directionality, base insertion and omission, is delicate to the relative focus ratio of dNTPs specified by the RNA template slippage-prone sequence and its 5′ adjoining base. The retrotransposon-derived enzyme TGIRT reveals extra slippage in vitro than the retroviral enzymes examined together with that from HIV.

Construction-mediated slippage could also be exhibited by different polymerases and enrich gene expression. A cassette from Drosophila retrotransposon Dme1_chrX_2630566, a candidate for using slippage for its GagPol synthesis, reveals sturdy slippage in vitro. Given the widespread incidence and significance of retrotransposons, systematic research to disclose the extent of their purposeful utilization of RT slippage are merited.

In Vitro Synthesized RNA Generated from cDNA Clones of Each Genomic Elements of Cucurbit yellow stunting dysfunction virus Replicates in Cucumber Protoplasts.

Cucurbit yellow stunting dysfunction virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a serious risk to industrial cucurbit manufacturing worldwide. Right here, development of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts in a position to replicate in cucumber protoplasts. CYSDV RNA1 proved competent for replication; transcription of each polarities of the genomic RNA was detectable 24 h submit inoculation.

Hybridization of complete RNA extracted from transfected protoplasts or from naturally CYSDV-infected cucurbits revealed high-level transcription of the p22 subgenomic RNA species. Replication of CYSDV RNA2 following co-transfection with RNA1 was additionally noticed, with comparable transcription kinetics. A CYSDV RNA2 cDNA clone (T3CM8Δ) comprising the 5′- and three’-UTRs plus the three’-terminal gene, generated a 2.eight kb RNA in a position to replicate to excessive ranges in protoplasts within the presence of CYSDV RNA1. The clone T3CM8Δ will facilitate reverse genetics research of CYSDV gene operate and RNA replication determinants.

A single-plasmid reverse genetics system for the rescue of non-segmented negative-strand RNA viruses from cloned full-length cDNA.

Reverse genetics techniques for non-segmented negative-strand RNA viruses depend on co-transfection of a plasmid containing the full-length viral cDNA and helper plasmids encoding important viral replication proteins. Right here, a system is offered wherein virus will be rescued from a single plasmid with out the necessity for helper plasmids in cells contaminated with a host-restricted recombinant poxvirus that expresses T7 RNA polymerase.

Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit

SED143Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in Tissue homogenates and other biological fluids.

Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit

4-SED143Hu
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Lenti ORF clone of Chrna3 (mGFP-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 3 (Chrna3), (10 ug)

RR210212L4 10 µg Ask for price

CHRNA3 cloning plasmid

CSB-CL005389HU1-10ug 10ug
EUR 451.2
Description: A cloning plasmid for the CHRNA3 gene.

CHRNA3 cloning plasmid

CSB-CL005389HU2-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the CHRNA3 gene.

Chrna3/ Rat Chrna3 ELISA Kit

ELI-49491r 96 Tests
EUR 1063.2

Lenti ORF clone of Chrna3 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3)

MR226689L3 10 µg Ask for price

Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3)

PAD143Hu01 100ul
EUR 245

Lenti ORF clone of Chrna3 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 3 (Chrna3), (10 ug)

RR210212L3 10 µg Ask for price

CHRNA3

CSB-CL005389HU1 10 μg plasmid + 200μl Glycerol Ask for price

CHRNA3

CSB-CL005389HU2 10 μg plasmid + 200μl Glycerol Ask for price

CHRNA3

pro-2216 5µg
EUR 60
Description: Recombinant Human Cholinergic Receptor Nicotinic Alpha 3

Lenti-ORF clone of CHRNA3 (mGFP-tagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 2

RC230168L4 10 µg Ask for price

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse)

4-PAD143Hu01
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3)

Lenti-ORF clone of CHRNA3 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 2

RC230168L3 10 µg Ask for price

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), PE

4-PAD143Hu01-PE
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with PE.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), APC

4-PAD143Hu01-APC
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with APC.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), Cy3

4-PAD143Hu01-Cy3
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with Cy3.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), FITC

4-PAD143Hu01-FITC
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with FITC.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), HRP

4-PAD143Hu01-HRP
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with HRP.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), Biotinylated

4-PAD143Hu01-Biotin
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with Biotin.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), APC-Cy7

4-PAD143Hu01-APC-Cy7
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with APC-Cy7.

CHRNA3 siRNA

20-abx911778
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

CHRNA3 siRNA

20-abx911779
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

CHRNA3 protein

80R-4244 100 ug
EUR 367
Description: Recombinant Human CHRNA3 protein with His tag

CHRNA3 antibody

20R-1327 100 ug
EUR 407
Description: Rabbit polyclonal CHRNA3 antibody

CHRNA3 Antibody

31245 100ul
EUR 319

CHRNA3 Antibody

31245-100ul 100ul
EUR 302.4

CHRNA3 Antibody

31245-50ul 50ul
EUR 224.4

CHRNA3 Antibody

E031245 100μg/100μl
EUR 255
Description: Available in various conjugation types.

CHRNA3 Antibody

E10-31232 100ul
EUR 225
Description: Available in various conjugation types.

CHRNA3 Antibody

E312636 200ul
EUR 275
Description: Available in various conjugation types.

CHRNA3 Antibody

E93040 100μg
EUR 255
Description: Available in various conjugation types.

CHRNA3 Antibody

GWB-24C7C5 0.1 mg Ask for price

CHRNA3 Antibody

GWB-3B0E87 0.05 mg Ask for price

CHRNA3 Antibody

E310823 200ul
EUR 275
Description: Available in various conjugation types.

CHRNA3 Antibody

1-CSB-PA000802
  • Ask for price
  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against CHRNA3. Recognizes CHRNA3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000

CHRNA3 Antibody

1-CSB-PA005389GA01HU
  • Ask for price
  • Ask for price
  • 150ul
  • 50ul
Description: A polyclonal antibody against CHRNA3. Recognizes CHRNA3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

CHRNA3 antibody

70R-16408 50 ul
EUR 289
Description: Rabbit polyclonal CHRNA3 antibody

CHRNA3 antibody

70R-49550 100 ul
EUR 242
Description: Purified Polyclonal CHRNA3 antibody

CHRNA3 antibody

70R-10537 50 ug
EUR 467
Description: Affinity purified rabbit polyclonal CHRNA3 antibody

CHRNA3 antibody

70R-5188 50 ug
EUR 467
Description: Rabbit polyclonal CHRNA3 antibody raised against the N terminal of CHRNA3

CHRNA3 Antibody

1-CSB-PA085715
  • Ask for price
  • Ask for price
  • 100ul
  • 50ul
Description: A polyclonal antibody against CHRNA3. Recognizes CHRNA3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:25-1:100

CHRNA3 Antibody

RQ4415 100 ug
EUR 356.15
Description: After binding acetylcholine, Nicotinic Acetylcholine Receptor alpha 3 (CHRNA3) responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. [UniProt]

CHRNA3 Antibody

GWB-ML996F 50ug Ask for price

CHRNA3 Mouse mAb

E2222178 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

CHRNA3 Rabbit pAb

E2160644 100ul
EUR 225
Description: Available in various conjugation types.

CHRNA3 Rabbit pAb

A1674-100ul 100 ul
EUR 369.6

CHRNA3 Rabbit pAb

A1674-200ul 200 ul
EUR 550.8

CHRNA3 Rabbit pAb

A1674-20ul 20 ul
EUR 219.6

CHRNA3 Rabbit pAb

A1674-50ul 50 ul
EUR 267.6

anti- CHRNA3 antibody

FNab01676 100µg
EUR 658.5
Description: Antibody raised against CHRNA3

CHRNA6 (untagged)-Human cholinergic receptor, nicotinic, alpha polypeptide 6 (cDNA clone MGC:23050 IMAGE:4862198), complete cds

SC123631 10 µg Ask for price

CHRNA3 Blocking Peptide

33R-2458 100 ug
EUR 119
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CHRNA3 antibody, catalog no. 70R-5188

CHRNA3 Blocking Peptide

33R-7520 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CHRNA3 antibody, catalog no. 20R-1327

CHRNA3 Blocking Peptide

20-abx062425
  • Ask for price
  • Ask for price
  • 1 mg
  • 5 mg

CHRNA3 Polyclonal Antibody

E91674 100ul
EUR 225
Description: Available in various conjugation types.

CHRNA3 Conjugated Antibody

C31245 100ul
EUR 476.4

CHRNA3 Polyclonal Antibody

E-AB-15792-120uL 120uL
EUR 240
Description: Unconjugated

CHRNA3 Polyclonal Antibody

E-AB-15792-200uL 200uL
EUR 399
Description: Unconjugated

CHRNA3 Polyclonal Antibody

E-AB-15792-20uL 20uL
EUR 73
Description: Unconjugated

CHRNA3 Polyclonal Antibody

E-AB-15792-60uL 60uL
EUR 143
Description: Unconjugated

CHRNA3 Polyclonal Antibody

E-AB-91625-120uL 120uL
EUR 320
Description: Unconjugated

CHRNA3 Polyclonal Antibody

E-AB-91625-200uL 200uL
EUR 530
Description: Unconjugated

CHRNA3 Polyclonal Antibody

E-AB-91625-60uL 60uL
EUR 200
Description: Unconjugated

CHRNA3 Polyclonal Antibody

E-AB-91625-each each Ask for price
Description: Unconjugated

Human CHRNA3 ELISA KIT

ELI-49490h 96 Tests
EUR 988.8

CHRNA3 ELISA KIT|Human

EF008656 96 Tests
EUR 826.8

Mouse Chrna3 ELISA KIT

ELI-11878m 96 Tests
EUR 1038

Bovine CHRNA3 ELISA KIT

ELI-49438b 96 Tests
EUR 1113.6

Chicken CHRNA3 ELISA KIT

ELI-24546c 96 Tests
EUR 1113.6

Chrna2 (untagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal) (cDNA clone MGC:18795 IMAGE:4193582), complete, (10ug)

MC206498 10 µg Ask for price

Human CHRNA3 shRNA Plasmid

20-abx950813
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

Mouse CHRNA3 shRNA Plasmid

20-abx980106
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

3`UTR clone of cholinergic receptor nicotinic alpha 3 (CHRNA3) for miRNA target validation

SC213784 10 µg Ask for price

pcDNA3.1(+)-CHRNA3(P229H) Plasmid

PVTB00094-2a 2 ug
EUR 427.2

Chrna3 (untagged ORF) - Rat cholinergic receptor, nicotinic, alpha 3 (Chrna3), (10 ug)

RN210212 10 µg Ask for price

Chrna3 (untagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3), (10ug)

MC217192 10 µg Ask for price

CHRNA3 Recombinant Protein (Rat)

RP194945 100 ug Ask for price

OACD00943-1ML - CHRNA3 Antibody

OACD00943-1ML 1ml
EUR 669

CHRNA3 Recombinant Protein (Mouse)

RP124064 100 ug Ask for price

CHRNA3 Recombinant Protein (Human)

RP007060 100 ug Ask for price

CHRNA3 Recombinant Protein (Human)

RP007063 100 ug Ask for price

OAGA02550-100UL - CHRNA3 Antibody

OAGA02550-100UL 100ul
EUR 369

OACD00943-100UL - CHRNA3 Antibody

OACD00943-100UL 100ul
EUR 199

CHRNA3 Rabbit Polyclonal Antibody

E10G04128 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

CHRNA3 Rabbit Polyclonal Antibody

E10G11049 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Chrna3 ORF Vector (Rat) (pORF)

ORF064983 1.0 ug DNA
EUR 607.2

CHRNA3 (untagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1

SC122160 10 µg Ask for price

CHRNA3 (untagged)-Human cholinergic receptor nicotinic alpha 3 (CHRNA3) transcript variant 2

SC328806 10 µg Ask for price

Chrna3 (GFP-tagged) - Mouse cholinergic receptor nicotinic alpha polypeptide 3 (Chrna3), (10ug)

MG226689 10 µg Ask for price

Chrna3 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3)

MR226689 10 µg Ask for price

Chrna3 ORF Vector (Mouse) (pORF)

ORF041356 1.0 ug DNA
EUR 607.2

CHRNA3 ORF Vector (Human) (pORF)

ORF002354 1.0 ug DNA
EUR 114

CHRNA3 ORF Vector (Human) (pORF)

ORF002355 1.0 ug DNA
EUR 114

Human CHRNA3 knockout cell line

ABC-KH3079 1 vial Ask for price
Description: Human CHRNA3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Chrna3 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 3 (Chrna3), (10 ug)

RR210212 10 µg Ask for price

Human CHRNA3 knockdown cell line

ABC-KD3079 1 vial Ask for price
Description: Human CHRNA3 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

CHRNA3 ELISA Kit (Human) (OKCD00894)

OKCD00894 96 Wells
EUR 997.2
Description: Description of target: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.107 ng/mL

CHRNA3 (GFP-tagged) - Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1

RG212582 10 µg Ask for price

CHRNA3 (GFP-tagged) - Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 2

RG230168 10 µg Ask for price

Mouse Monoclonal Antibody to CHRNA3

sAP-1563 50ug
EUR 270

Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, mGFP tagged

RC212582L2 10 µg Ask for price

Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, mGFP tagged

RC212582L4 10 µg Ask for price

CHRNA3 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 2

RC230168 10 µg Ask for price

CHRNA3 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1

RC212582 10 µg Ask for price

CHRNA3 rabbit polyclonal antibody, Serum

AP55107SU-N 200 µl Ask for price

OPCD00631-10UG - CHRNA3 Recombinant Protein

OPCD00631-10UG 10ug
EUR 99

OPCD00631-50UG - CHRNA3 Recombinant Protein

OPCD00631-50UG 50ug
EUR 239

OPCD00631-200UG - CHRNA3 Recombinant Protein

OPCD00631-200UG 200ug
EUR 469

OKCD00894-96W - CHRNA3 ELISA Kit (Human)

OKCD00894-96W 96Wells
EUR 675

Chrna3 sgRNA CRISPR Lentivector set (Rat)

K6844801 3 x 1.0 ug
EUR 406.8

Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, Myc-DDK-tagged

RC212582L1 10 µg Ask for price

Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, Myc-DDK-tagged

RC212582L3 10 µg Ask for price

Chrna3 3'UTR GFP Stable Cell Line

TU153869 1.0 ml Ask for price

CHRNA3 3'UTR GFP Stable Cell Line

TU054393 1.0 ml
EUR 1825.2

Chrna3 3'UTR GFP Stable Cell Line

TU252321 1.0 ml Ask for price

Chrna3 sgRNA CRISPR Lentivector set (Mouse)

K3739501 3 x 1.0 ug
EUR 406.8

CHRNA3 sgRNA CRISPR Lentivector set (Human)

K0448801 3 x 1.0 ug
EUR 406.8

Rabbit polyclonal CHRNA3 Antibody (N-term)

TA324798 400 µl Ask for price

CHRNA3 Protein Vector (Rat) (pPM-C-HA)

PV259932 500 ng
EUR 723.6

CHRNA3 Protein Vector (Rat) (pPB-C-His)

PV259930 500 ng
EUR 723.6

CHRNA3 Protein Vector (Rat) (pPB-N-His)

PV259931 500 ng
EUR 723.6

CHRNA3 Protein Vector (Rat) (pPM-C-His)

PV259933 500 ng
EUR 723.6

Lenti ORF particles, Chrna3 (GFP-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 3 (Chrna3), 200ul, >10^7 TU/mL

RR210212L4V 200 µl Ask for price

CHRNA3 Protein Vector (Human) (pPM-C-HA)

PV009415 500 ng
EUR 394.8

CHRNA3 Protein Vector (Human) (pPM-C-HA)

PV009419 500 ng
EUR 394.8

CHRNA3 Protein Vector (Mouse) (pPM-C-HA)

PV165424 500 ng
EUR 723.6

CHRNA3 Protein Vector (Human) (pPB-C-His)

PV009413 500 ng
EUR 394.8

CHRNA3 Protein Vector (Human) (pPB-N-His)

PV009414 500 ng
EUR 394.8

CHRNA3 Protein Vector (Human) (pPM-C-His)

PV009416 500 ng
EUR 394.8

CHRNA3 Protein Vector (Human) (pPB-C-His)

PV009417 500 ng
EUR 394.8

CHRNA3 Protein Vector (Human) (pPB-N-His)

PV009418 500 ng
EUR 394.8

CHRNA3 Protein Vector (Human) (pPM-C-His)

PV009420 500 ng
EUR 394.8

CHRNA3 Protein Vector (Mouse) (pPB-C-His)

PV165422 500 ng
EUR 723.6

CHRNA3 Protein Vector (Mouse) (pPB-N-His)

PV165423 500 ng
EUR 723.6

CHRNA3 Protein Vector (Mouse) (pPM-C-His)

PV165425 500 ng
EUR 723.6

Chrna3 3'UTR Luciferase Stable Cell Line

TU103869 1.0 ml Ask for price

CHRNA3 3'UTR Luciferase Stable Cell Line

TU004393 1.0 ml
EUR 1825.2

Chrna3 3'UTR Luciferase Stable Cell Line

TU202321 1.0 ml Ask for price

OPCA05083-1MG - CHRNA3 Recombinant Protein (Human)

OPCA05083-1MG 1mg
EUR 1379

OPCA05083-20UG - CHRNA3 Recombinant Protein (Human)

OPCA05083-20UG 20ug
EUR 218

OPCA05083-100UG - CHRNA3 Recombinant Protein (Human)

OPCA05083-100UG 100ug
EUR 323

Lenti ORF particles, Chrna3 (GFP-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3), 200ul, >10^7 TU/mL

MR226689L4V 200 µl Ask for price

Lenti ORF particles, Chrna3 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 3 (Chrna3), 200ul, >10^7 TU/mL

RR210212L3V 200 µl Ask for price

Chrna3 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6844802 1.0 ug DNA
EUR 184.8

Chrna3 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6844803 1.0 ug DNA
EUR 184.8

Chrna3 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6844804 1.0 ug DNA
EUR 184.8

Chrna3 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3739502 1.0 ug DNA
EUR 184.8

Chrna3 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3739503 1.0 ug DNA
EUR 184.8

Chrna3 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3739504 1.0 ug DNA
EUR 184.8

CHRNA3 sgRNA CRISPR Lentivector (Human) (Target 1)

K0448802 1.0 ug DNA
EUR 184.8

CHRNA3 sgRNA CRISPR Lentivector (Human) (Target 2)

K0448803 1.0 ug DNA
EUR 184.8

CHRNA3 sgRNA CRISPR Lentivector (Human) (Target 3)

K0448804 1.0 ug DNA
EUR 184.8

Lenti ORF particles, Chrna3 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3), 200ul, >10^7 TU/mL

MR226689L3V 200 µl Ask for price

AAP34964-100UG - CHRNA3 Peptide - N-terminal region

AAP34964-100UG 100ug
EUR 99

ARP34964_P050-25UL - CHRNA3 Antibody - N-terminal region

ARP34964_P050-25UL 25ul
EUR 99

OAAB02436-400UL - CHRNA3 Antibody - N-terminal region

OAAB02436-400UL 400ul
EUR 409

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNA3) Antibody

20-abx327182
  • Ask for price
  • Ask for price
  • 100 ug
  • 50 ug

AVARP13014_P050-25UL - CHRNA3 Antibody - N-terminal region

AVARP13014_P050-25UL 25ul
EUR 99

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Antibody

20-abx130886
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Antibody

20-abx171728
  • Ask for price
  • Ask for price
  • 1 mg
  • 200 ug

Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody

20-abx125675
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody

abx231676-100ug 100 ug
EUR 610.8

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNA3) Antibody

20-abx241009
  • Ask for price
  • Ask for price
  • 100 ul
  • 50 ul

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNA3) Antibody

20-abx111610
  • Ask for price
  • Ask for price
  • 150 ul
  • 50 ul

This method depends on the insertion of T7 promoter sequences within the viral cDNA at positions that enable transcription of sub-genomic RNAs encoding important viral replication proteins.

Leave a Reply

Your email address will not be published. Required fields are marked *

Related Post

The signal transducer CD24 suppresses the germ cell program and promotes an ectodermal rather than mesodermal cell fate in embryonal carcinomas

The signal transducer CD24 suppresses the germ cell program and promotes an ectodermal rather than mesodermal cell fate in embryonal carcinomasThe signal transducer CD24 suppresses the germ cell program and promotes an ectodermal rather than mesodermal cell fate in embryonal carcinomas

Testicular germ cell tumors (GCTs) are stratified into seminomas and non-seminomas. Seminomas share many histological and molecular options with primordial germ cells, whereas the non-seminoma stem cell inhabitants – embryonal

Sero-diagnostic efficacy of various ELISA kits for diagnosis of infectious bovine rhinotracheitis (IBR) in cattle and buffaloes in India

Sero-diagnostic efficacy of various ELISA kits for diagnosis of infectious bovine rhinotracheitis (IBR) in cattle and buffaloes in IndiaSero-diagnostic efficacy of various ELISA kits for diagnosis of infectious bovine rhinotracheitis (IBR) in cattle and buffaloes in India

Bovine alphaherpesvirus-1 (BoHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is an economically vital viral pathogen affecting cattle and buffaloes. Serological assays are largely used for detection of the antibodies,

Novel Symbiotic Genome-Scale Model Reveals Wolbachia's Arboviral Pathogen Blocking Mechanism in Aedes aegypti

Novel Symbiotic Genome-Scale Model Reveals Wolbachia’s Arboviral Pathogen Blocking Mechanism in Aedes aegyptiNovel Symbiotic Genome-Scale Model Reveals Wolbachia’s Arboviral Pathogen Blocking Mechanism in Aedes aegypti

Wolbachia are endosymbiont micro organism recognized to contaminate arthropods inflicting totally different results, resembling cytoplasmic incompatibility and pathogen blocking in Aedes aegypti. Though a number of Wolbachia strains have been studied, there may