Entire genome large evaluation of transcription utilizing RNA-Seq strategies is a strong strategy to elucidate differential expression of gene options in micro organism throughout completely different circumstances in addition to for locating beforehand unique RNA species.
Certainly, RNA sequencing has revolutionized the examine of bacterial transcription with the range and amount of small noncoding RNA parts which were discovered and its capability to obviously outline operons, promoters , and terminators . We focus on our expertise with making use of RNA sequencing know-how to analyzing the lytic cycle, together with extraction, processing, and a information to the custom-made statistical evaluation needed for analyzing differential host and phage transcription.
Speedy Development of Advanced Plant RNA Virus Infectious cDNA Clones for Agroinfection Utilizing a Yeast-E. coli-Agrobacterium Shuttle Vector.
The provision of infectious full-length clone is indispensable for reverse genetics research of virus biology, pathology and development of viral vectors. Nevertheless, for RNA viruses with giant genome sizes or these exhibiting inherent cloning difficulties, process to generate biologically lively round DNA (cDNA) clones will be time-consuming or technically difficult. Right here we have now constructed a yeast-Escherichia coli-Agrobacterium shuttle vector that permits extremely environment friendly homologous recombination in yeast for meeting of Agrobacterium suitable plant virus clones. Utilizing this vector, we present that infectious cDNA clones of a plant negative-stranded RNA virus, sonchus yellow web rhabdovirus, will be quickly assembled. As well as, one-step meeting of infectious clones of potato virus Y in yeast, both with or with out intron, was readily achieved from as many as eight overlapping DNA fragments.
Extra importantly, the recovered yeast plasmids will be remodeled immediately into Agrobacterium for inoculation, thereby obviating the E. coli cloning steps and related toxicity points. This technique is speedy, extremely environment friendly and cost-effective and needs to be readily relevant to a broad vary of plant viruses.
3′ RNA ligase mediated speedy amplification of cDNA ends for validating viroid induced cleavage on the 3′ extremity of the host mRNA.
5′ RNA ligase-mediated speedy amplification of cDNA ends (5′ RLM-RACE) is a widely-accepted technique for the validation of direct cleavage of a goal gene by a microRNA (miRNA) and viroid-derived small RNA (vd-sRNA). Nevertheless, this technique can’t be used if cleavage takes place within the 3′ extremity of the goal RNA, as this offers inadequate sequence size to design nested PCR primers for five’ RLM RACE. To beat this hurdle, we have now developed 3′ RNA ligase-mediated speedy amplification of cDNA ends (3′ RLM RACE).
On this technique, an oligonucleotide adapter having 5′ adenylated and three’ blocked is ligated to the three’ finish of the cleaved RNA adopted by PCR amplification utilizing gene particular primers. In different phrases, in 3′ RLM RACE, 3′ finish is mapped utilizing 5′ fragment as a substitute of small 3′ fragment.

The strategy developed right here was verified by inspecting the bioinformatics predicted and parallel evaluation of RNA ends (PARE) proved cleavage websites of chloride channel protein CLC-b-like mRNA in Potato spindle tuber viroid contaminated tomato vegetation. The three’ RLM RACE developed on this examine has the potential to validate the miRNA and vd-sRNA mediated cleavage of mRNAs at its 3′ untranslated area (3′ UTR).
Stimulation of reverse transcriptase generated cDNAs with particular indels by template RNA construction: retrotransposon, dNTP steadiness, RT-reagent utilization.
RNA dependent DNA-polymerases, reverse transcriptases, are key enzymes for retroviruses and retroelements. Their constancy, together with indel technology, is important for his or her use as reagents together with for deep sequencing. Right here, we report that sure RNA template buildings and G-rich sequences, forward of numerous reverse transcriptases will be sturdy stimulators for slippage at slippage-prone template motif sequence 3′ of such ‘slippage-stimulatory’ buildings. The place slippage is stimulated, the ensuing merchandise have a number of extra base(s) in comparison with the corresponding template motif.
Such buildings additionally inhibit slippage-mediated base omission which will be extra frequent within the absence of a related stem-loop. Slippage directionality, base insertion and omission, is delicate to the relative focus ratio of dNTPs specified by the RNA template slippage-prone sequence and its 5′ adjoining base. The retrotransposon-derived enzyme TGIRT reveals extra slippage in vitro than the retroviral enzymes examined together with that from HIV.
Construction-mediated slippage could also be exhibited by different polymerases and enrich gene expression. A cassette from Drosophila retrotransposon Dme1_chrX_2630566, a candidate for using slippage for its GagPol synthesis, reveals sturdy slippage in vitro. Given the widespread incidence and significance of retrotransposons, systematic research to disclose the extent of their purposeful utilization of RT slippage are merited.
In Vitro Synthesized RNA Generated from cDNA Clones of Each Genomic Elements of Cucurbit yellow stunting dysfunction virus Replicates in Cucumber Protoplasts.
Cucurbit yellow stunting dysfunction virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a serious risk to industrial cucurbit manufacturing worldwide. Right here, development of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts in a position to replicate in cucumber protoplasts. CYSDV RNA1 proved competent for replication; transcription of each polarities of the genomic RNA was detectable 24 h submit inoculation.
Hybridization of complete RNA extracted from transfected protoplasts or from naturally CYSDV-infected cucurbits revealed high-level transcription of the p22 subgenomic RNA species. Replication of CYSDV RNA2 following co-transfection with RNA1 was additionally noticed, with comparable transcription kinetics. A CYSDV RNA2 cDNA clone (T3CM8Δ) comprising the 5′- and three’-UTRs plus the three’-terminal gene, generated a 2.eight kb RNA in a position to replicate to excessive ranges in protoplasts within the presence of CYSDV RNA1. The clone T3CM8Δ will facilitate reverse genetics research of CYSDV gene operate and RNA replication determinants.
A single-plasmid reverse genetics system for the rescue of non-segmented negative-strand RNA viruses from cloned full-length cDNA.
Reverse genetics techniques for non-segmented negative-strand RNA viruses depend on co-transfection of a plasmid containing the full-length viral cDNA and helper plasmids encoding important viral replication proteins. Right here, a system is offered wherein virus will be rescued from a single plasmid with out the necessity for helper plasmids in cells contaminated with a host-restricted recombinant poxvirus that expresses T7 RNA polymerase.
CHRNA3 antibody |
70R-10537 |
Fitzgerald |
50 ug |
EUR 560.4 |
Description: Affinity purified rabbit polyclonal CHRNA3 antibody |
CHRNA3 antibody |
70R-49550 |
Fitzgerald |
100 ul |
EUR 292.8 |
Description: Purified Polyclonal CHRNA3 antibody |
CHRNA3 antibody |
70R-16408 |
Fitzgerald |
50 ul |
EUR 522 |
Description: Rabbit polyclonal CHRNA3 antibody |
CHRNA3 antibody |
70R-5188 |
Fitzgerald |
50 ug |
EUR 560.4 |
Description: Rabbit polyclonal CHRNA3 antibody raised against the N terminal of CHRNA3 |
CHRNA3 antibody |
20R-1327 |
Fitzgerald |
100 ug |
EUR 452.4 |
Description: Rabbit polyclonal CHRNA3 antibody |
CHRNA3 Antibody |
31245-100ul |
SAB |
100ul |
EUR 302.4 |
CHRNA3 Antibody |
31245-50ul |
SAB |
50ul |
EUR 224.4 |
CHRNA3 siRNA |
20-abx911778 |
Abbexa |
|
|
|
CHRNA3 siRNA |
20-abx911779 |
Abbexa |
|
|
|
CHRNA3 protein |
80R-4244 |
Fitzgerald |
100 ug |
EUR 418.8 |
Description: Recombinant Human CHRNA3 protein with His tag |
CHRNA3 Antibody |
1-CSB-PA000802 |
Cusabio |
|
|
|
Description: A polyclonal antibody against CHRNA3. Recognizes CHRNA3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000 |
CHRNA3 Antibody |
1-CSB-PA085715 |
Cusabio |
|
|
|
Description: A polyclonal antibody against CHRNA3. Recognizes CHRNA3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:25-1:100 |
CHRNA3 Antibody |
1-CSB-PA005389GA01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against CHRNA3. Recognizes CHRNA3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC |
Anti-ASFV P72 Antibody | MASF-5A-Clone #10E11 |
MASF-5A-Clone-10E11 |
Immunology Consultants Laboratory |
1 mg |
EUR 667 |
Description: Anti-ASFV P72 Antibody | MASF-5A-Clone #10E11 | Immunology Consultants Laboratory Host: Mouse Format: Unconjugated AP Product Type: Primary Antibody Antibody Clonality: Monoclonal |
Anti-ASFV P72 Antibody | MASF-5A-Clone #10G5 |
MASF-5A-Clone-10G5 |
Immunology Consultants Laboratory |
1 mg |
EUR 667 |
Description: Anti-ASFV P72 Antibody | MASF-5A-Clone #10G5 | Immunology Consultants Laboratory Host: Mouse Format: Unconjugated AP Product Type: Primary Antibody Antibody Clonality: Monoclonal |
Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#3B11 |
MALV-30A-Clone-3B11 |
Immunology Consultants Laboratory |
1.0 mg |
EUR 448 |
Description: Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#3B11 | Immunology Consultants Laboratory Host: Mouse Format: Unconjugated AP Product Type: Primary Antibody Antibody Clonality: Monoclonal |
Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#5A4 |
MALV-30A-Clone-5A4 |
Immunology Consultants Laboratory |
1.0 mg |
EUR 448 |
Description: Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#5A4 | Immunology Consultants Laboratory Host: Mouse Format: Unconjugated AP Product Type: Primary Antibody Antibody Clonality: Monoclonal |
Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6C2 |
MALV-30A-Clone-6C2 |
Immunology Consultants Laboratory |
1.0 mg |
EUR 448 |
Description: Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6C2 | Immunology Consultants Laboratory Host: Mouse Format: Unconjugated AP Product Type: Primary Antibody Antibody Clonality: Monoclonal |
Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6D7 |
MALV-30A-Clone-6D7 |
Immunology Consultants Laboratory |
1.0 mg |
EUR 448 |
Description: Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6D7 | Immunology Consultants Laboratory Host: Mouse Format: Unconjugated AP Product Type: Primary Antibody Antibody Clonality: Monoclonal |
CHRNA3 Blocking Peptide |
33R-2458 |
Fitzgerald |
100 ug |
EUR 216 |
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CHRNA3 antibody, catalog no. 70R-5188 |
CHRNA3 Blocking Peptide |
33R-7520 |
Fitzgerald |
100 ug |
EUR 216 |
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CHRNA3 antibody, catalog no. 20R-1327 |
CHRNA3 Blocking Peptide |
20-abx062425 |
Abbexa |
|
|
|
CHRNA3 Rabbit pAb |
A1674-100ul |
Abclonal |
100 ul |
EUR 369.6 |
CHRNA3 Rabbit pAb |
A1674-200ul |
Abclonal |
200 ul |
EUR 550.8 |
CHRNA3 Rabbit pAb |
A1674-20ul |
Abclonal |
20 ul |
EUR 219.6 |
CHRNA3 Rabbit pAb |
A1674-50ul |
Abclonal |
50 ul |
EUR 267.6 |
CHRNA3 Conjugated Antibody |
C31245 |
SAB |
100ul |
EUR 476.4 |
CHRNA3 cloning plasmid |
CSB-CL005389HU1-10ug |
Cusabio |
10ug |
EUR 451.2 |
|
Description: A cloning plasmid for the CHRNA3 gene. |
CHRNA3 cloning plasmid |
CSB-CL005389HU2-10ug |
Cusabio |
10ug |
EUR 279.6 |
|
Description: A cloning plasmid for the CHRNA3 gene. |
anti- CHRNA3 antibody |
FNab01676 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against CHRNA3 |
Anti-CHRNA3 antibody |
STJ111103 |
St John's Laboratory |
100 µl |
EUR 332.4 |
Description: This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. |
Recombinant Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) |
4-RPD143Hu01 |
Cloud-Clone |
-
EUR 442.56
-
EUR 242.40
-
EUR 1329.60
-
EUR 523.20
-
EUR 926.40
-
EUR 372.00
-
EUR 3144.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Cholinergic Receptor, Nicotinic, Alpha 3 expressed in: E.coli |
Human CHRNA3 shRNA Plasmid |
20-abx950813 |
Abbexa |
|
|
|
Mouse CHRNA3 shRNA Plasmid |
20-abx980106 |
Abbexa |
|
|
|
CHRNA3 Recombinant Protein (Human) |
RP007060 |
ABM |
100 ug |
Ask for price |
CHRNA3 Recombinant Protein (Human) |
RP007063 |
ABM |
100 ug |
Ask for price |
CHRNA3 Recombinant Protein (Rat) |
RP194945 |
ABM |
100 ug |
Ask for price |
CHRNA3 Recombinant Protein (Mouse) |
RP124064 |
ABM |
100 ug |
Ask for price |
Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit |
SED143Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5677.8 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in Tissue homogenates and other biological fluids. |
Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit |
SED143Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 572.76 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in Tissue homogenates and other biological fluids. |
Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit |
SED143Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 766.8 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in Tissue homogenates and other biological fluids. |
Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit |
SED143Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3090.6 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in Tissue homogenates and other biological fluids. |
Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit |
4-SED143Hu |
Cloud-Clone |
-
EUR 5738.40
-
EUR 3031.20
-
EUR 768.00
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
Novo? cDNA Kit |
M1165-100 |
Biovision |
each |
EUR 424.8 |
Novo? cDNA Kit |
M1165-25 |
Biovision |
each |
EUR 320.4 |
Evo? cDNA Supermix |
M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
each |
EUR 320.4 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
each |
EUR 346.8 |
Chrna3 ORF Vector (Rat) (pORF) |
ORF064983 |
ABM |
1.0 ug DNA |
EUR 607.2 |
CHRNA3 ORF Vector (Human) (pORF) |
ORF002354 |
ABM |
1.0 ug DNA |
EUR 114 |
CHRNA3 ORF Vector (Human) (pORF) |
ORF002355 |
ABM |
1.0 ug DNA |
EUR 114 |
Chrna3 ORF Vector (Mouse) (pORF) |
ORF041356 |
ABM |
1.0 ug DNA |
EUR 607.2 |
CHRNA3 ELISA Kit (Human) (OKCD00894) |
OKCD00894 |
Aviva Systems Biology |
96 Wells |
EUR 997.2 |
Description: Description of target: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.107 ng/mL |
cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis |
C1634310 |
Biochain |
40 reactions |
EUR 745.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Plant Normal Tissue: cDNA from Plant: Corn |
C1634330 |
Biochain |
40 reactions |
EUR 745.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Plant Normal Tissue: cDNA from Plant: Orange |
C1634340 |
Biochain |
40 reactions |
EUR 745.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Plant Normal Tissue: cDNA from Plant: Potato |
C1634350 |
Biochain |
40 reactions |
EUR 745.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Plant Normal Tissue: cDNA from Plant: Rice |
C1634360 |
Biochain |
40 reactions |
EUR 745.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Plant Normal Tissue: cDNA from Plant: Wheat |
C1634390 |
Biochain |
40 reactions |
EUR 745.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse) |
4-PAD143Hu01 |
Cloud-Clone |
-
EUR 296.40
-
EUR 3012.00
-
EUR 750.00
-
EUR 372.00
-
EUR 256.80
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) |
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
20-abx09801620ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
20-abx09802120ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean |
C1634370 |
Biochain |
40 reactions |
EUR 745.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Chrna3 sgRNA CRISPR Lentivector set (Rat) |
K6844801 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
CHRNA3 sgRNA CRISPR Lentivector set (Human) |
K0448801 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
Chrna3 sgRNA CRISPR Lentivector set (Mouse) |
K3739501 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), APC |
4-PAD143Hu01-APC |
Cloud-Clone |
-
EUR 414.00
-
EUR 3930.00
-
EUR 1094.40
-
EUR 528.00
-
EUR 262.80
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with APC. |
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), Biotinylated |
4-PAD143Hu01-Biotin |
Cloud-Clone |
-
EUR 373.20
-
EUR 2952.00
-
EUR 872.40
-
EUR 457.20
-
EUR 262.80
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with Biotin. |
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), Cy3 |
4-PAD143Hu01-Cy3 |
Cloud-Clone |
-
EUR 502.80
-
EUR 5190.00
-
EUR 1410.00
-
EUR 654.00
-
EUR 301.20
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with Cy3. |
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), FITC |
4-PAD143Hu01-FITC |
Cloud-Clone |
-
EUR 355.20
-
EUR 3168.00
-
EUR 900.00
-
EUR 446.40
-
EUR 234.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with FITC. |
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), HRP |
4-PAD143Hu01-HRP |
Cloud-Clone |
-
EUR 379.20
-
EUR 3426.00
-
EUR 968.40
-
EUR 477.60
-
EUR 247.20
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with HRP. |
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), PE |
4-PAD143Hu01-PE |
Cloud-Clone |
-
EUR 355.20
-
EUR 3168.00
-
EUR 900.00
-
EUR 446.40
-
EUR 234.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with PE. |
Tetro cDNA Synthesis Kit |
BIO-65042 |
Bioline |
30 Reactions |
Ask for price |
Tetro cDNA Synthesis Kit |
BIO-65043 |
Bioline |
100 Reactions |
Ask for price |
SensiFAST cDNA Synthesis Kit |
BIO-65053 |
Bioline |
50 Reactions |
Ask for price |
SensiFAST cDNA Synthesis Kit |
BIO-65053/S |
Bioline |
Sample |
Ask for price |
SensiFAST cDNA Synthesis Kit |
BIO-65054 |
Bioline |
250 Reactions |
Ask for price |
cDNA Probe Diluent Solution |
AR0063 |
BosterBio |
5mL |
EUR 127.2 |
cDNA from Arteriosclerosis: Aorta |
C1236012Hd-4 |
Biochain |
40 reactions |
EUR 973.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from hypertension: Artery |
C1236013Hd-2 |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Arteriosclerosis: Artery |
C1236013Hd-4 |
Biochain |
40 reactions |
EUR 973.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from hypertension: Vein |
C1236020Hd-2 |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Lupus: Colon |
C1236090Lup |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from hypertension: Heart |
C1236122Hd-2 |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Lupus: Heart |
C1236122Lup |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from hypertension: Kidney |
C1236142Hd-2 |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Lupus: Kidney |
C1236142Lup |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Lupus: Liver |
C1236149Lup |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Asthma: Lung |
C1236152Ld-1 |
Biochain |
40 reactions |
EUR 973.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Bronchitis: Lung |
C1236152Ld-2 |
Biochain |
40 reactions |
EUR 973.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Emphysema: Lung |
C1236152Ld-3 |
Biochain |
40 reactions |
EUR 973.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Pneumonia: Lung |
C1236152Ld-4 |
Biochain |
40 reactions |
EUR 973.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Lupus: Lung |
C1236152Lup |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Lupus: Pancreas |
C1236188Lup |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Lupus: Spleen |
C1236246Lup |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Lupus: stomach |
C1236248Lup |
Biochain |
40 reactions |
EUR 801.6 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
OneScriptPlus cDNA Synthesis Kit |
G235 |
ABM |
25 x 20 ul reactions |
EUR 116.4 |
OneScriptPlus cDNA Synthesis Kit |
G236 |
ABM |
100 x 20 ul reactions |
EUR 202.8 |
OneScriptPlus cDNA Synthesis SuperMix |
G453 |
ABM |
25 x 20 ul reactions |
EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
G454 |
ABM |
100 x 20 ul reactions |
EUR 202.8 |
circRNA cDNA Synthesis Kit |
G627 |
ABM |
25 rxn (20 ul/rxn) |
EUR 370.8 |
Evo™ cDNA Kit |
M1164-100 |
Biovision |
each |
Ask for price |
Evo™ cDNA Kit |
M1164-25 |
Biovision |
each |
Ask for price |
Novo? Transcriptome cDNA Kit |
M1167-100 |
Biovision |
each |
EUR 1142.4 |
Novo? Transcriptome cDNA Kit |
M1167-25 |
Biovision |
each |
EUR 529.2 |
EVery cDNA Synthesis Kit |
EVery200B-1 |
SBI |
20 preps |
EUR 307 |
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), APC-Cy7 |
4-PAD143Hu01-APC-Cy7 |
Cloud-Clone |
-
EUR 685.20
-
EUR 7716.00
-
EUR 2046.00
-
EUR 912.00
-
EUR 382.80
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with APC-Cy7. |
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Antibody |
20-abx171728 |
Abbexa |
|
|
|
Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody |
20-abx009435 |
Abbexa |
-
EUR 360.00
-
EUR 526.80
-
EUR 226.80
|
|
|
Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody |
20-abx123004 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 376.80
|
|
|
Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody |
20-abx125675 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 218.40
-
EUR 376.80
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Antibody |
20-abx130886 |
Abbexa |
-
EUR 510.00
-
EUR 159.60
-
EUR 1446.00
-
EUR 693.60
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNA3) Antibody |
20-abx111610 |
Abbexa |
|
|
|
Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody |
abx032382-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody |
abx032382-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNA3) Antibody |
20-abx327182 |
Abbexa |
|
|
|
Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody |
abx231676-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNA3) Antibody |
20-abx241009 |
Abbexa |
|
|
|
Chrna3 sgRNA CRISPR Lentivector (Rat) (Target 1) |
K6844802 |
ABM |
1.0 ug DNA |
EUR 184.8 |
Chrna3 sgRNA CRISPR Lentivector (Rat) (Target 2) |
K6844803 |
ABM |
1.0 ug DNA |
EUR 184.8 |
Chrna3 sgRNA CRISPR Lentivector (Rat) (Target 3) |
K6844804 |
ABM |
1.0 ug DNA |
EUR 184.8 |
CHRNA3 sgRNA CRISPR Lentivector (Human) (Target 1) |
K0448802 |
ABM |
1.0 ug DNA |
EUR 184.8 |
CHRNA3 sgRNA CRISPR Lentivector (Human) (Target 2) |
K0448803 |
ABM |
1.0 ug DNA |
EUR 184.8 |
CHRNA3 sgRNA CRISPR Lentivector (Human) (Target 3) |
K0448804 |
ABM |
1.0 ug DNA |
EUR 184.8 |
Chrna3 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
K3739502 |
ABM |
1.0 ug DNA |
EUR 184.8 |
Chrna3 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
K3739503 |
ABM |
1.0 ug DNA |
EUR 184.8 |
This method depends on the insertion of T7 promoter sequences within the viral cDNA at positions that enable transcription of sub-genomic RNAs encoding important viral replication proteins.