Preparing cDNA Libraries from Lytic Phage-Infected Cells for Whole Transcriptome Analysis by RNA-Seq.

Preparing cDNA Libraries from Lytic Phage-Infected Cells for Whole Transcriptome Analysis by RNA-Seq.

Entire genome large evaluation of transcription utilizing RNA-Seq strategies is a strong strategy to elucidate differential expression of gene options in micro organism throughout completely different circumstances in addition to for locating beforehand unique RNA species.

Certainly, RNA sequencing has revolutionized the examine of bacterial transcription with the range and amount of small noncoding RNA parts which were discovered and its capability to obviously outline operons, promoters , and terminators . We focus on our expertise with making use of RNA sequencing know-how to analyzing the lytic cycle, together with extraction, processing, and a information to the custom-made statistical evaluation needed for analyzing differential host and phage transcription.

Speedy Development of Advanced Plant RNA Virus Infectious cDNA Clones for Agroinfection Utilizing a Yeast-E. coli-Agrobacterium Shuttle Vector.

The provision of infectious full-length clone is indispensable for reverse genetics research of virus biology, pathology and development of viral vectors. Nevertheless, for RNA viruses with giant genome sizes or these exhibiting inherent cloning difficulties, process to generate biologically lively round DNA (cDNA) clones will be time-consuming or technically difficult. Right here we have now constructed a yeast-Escherichia coli-Agrobacterium shuttle vector that permits extremely environment friendly homologous recombination in yeast for meeting of Agrobacterium suitable plant virus clones. Utilizing this vector, we present that infectious cDNA clones of a plant negative-stranded RNA virus, sonchus yellow web rhabdovirus, will be quickly assembled. As well as, one-step meeting of infectious clones of potato virus Y in yeast, both with or with out intron, was readily achieved from as many as eight overlapping DNA fragments.

Extra importantly, the recovered yeast plasmids will be remodeled immediately into Agrobacterium for inoculation, thereby obviating the E. coli cloning steps and related toxicity points. This technique is speedy, extremely environment friendly and cost-effective and needs to be readily relevant to a broad vary of plant viruses.

3′ RNA ligase mediated speedy amplification of cDNA ends for validating viroid induced cleavage on the 3′ extremity of the host mRNA.

5′ RNA ligase-mediated speedy amplification of cDNA ends (5′ RLM-RACE) is a widely-accepted technique for the validation of direct cleavage of a goal gene by a microRNA (miRNA) and viroid-derived small RNA (vd-sRNA). Nevertheless, this technique can’t be used if cleavage takes place within the 3′ extremity of the goal RNA, as this offers inadequate sequence size to design nested PCR primers for five’ RLM RACE. To beat this hurdle, we have now developed 3′ RNA ligase-mediated speedy amplification of cDNA ends (3′ RLM RACE).

On this technique, an oligonucleotide adapter having 5′ adenylated and three’ blocked is ligated to the three’ finish of the cleaved RNA adopted by PCR amplification utilizing gene particular primers. In different phrases, in 3′ RLM RACE, 3′ finish is mapped utilizing 5′ fragment as a substitute of small 3′ fragment.

Preparing cDNA Libraries from Lytic Phage-Infected Cells for Whole Transcriptome Analysis by RNA-Seq.

The strategy developed right here was verified by inspecting the bioinformatics predicted and parallel evaluation of RNA ends (PARE) proved cleavage websites of chloride channel protein CLC-b-like mRNA in Potato spindle tuber viroid contaminated tomato vegetation. The three’ RLM RACE developed on this examine has the potential to validate the miRNA and vd-sRNA mediated cleavage of mRNAs at its 3′ untranslated area (3′ UTR).

Stimulation of reverse transcriptase generated cDNAs with particular indels by template RNA construction: retrotransposon, dNTP steadiness, RT-reagent utilization.

RNA dependent DNA-polymerases, reverse transcriptases, are key enzymes for retroviruses and retroelements. Their constancy, together with indel technology, is important for his or her use as reagents together with for deep sequencing. Right here, we report that sure RNA template buildings and G-rich sequences, forward of numerous reverse transcriptases will be sturdy stimulators for slippage at slippage-prone template motif sequence 3′ of such ‘slippage-stimulatory’ buildings. The place slippage is stimulated, the ensuing merchandise have a number of extra base(s) in comparison with the corresponding template motif.

Such buildings additionally inhibit slippage-mediated base omission which will be extra frequent within the absence of a related stem-loop. Slippage directionality, base insertion and omission, is delicate to the relative focus ratio of dNTPs specified by the RNA template slippage-prone sequence and its 5′ adjoining base. The retrotransposon-derived enzyme TGIRT reveals extra slippage in vitro than the retroviral enzymes examined together with that from HIV.

Construction-mediated slippage could also be exhibited by different polymerases and enrich gene expression. A cassette from Drosophila retrotransposon Dme1_chrX_2630566, a candidate for using slippage for its GagPol synthesis, reveals sturdy slippage in vitro. Given the widespread incidence and significance of retrotransposons, systematic research to disclose the extent of their purposeful utilization of RT slippage are merited.

In Vitro Synthesized RNA Generated from cDNA Clones of Each Genomic Elements of Cucurbit yellow stunting dysfunction virus Replicates in Cucumber Protoplasts.

Cucurbit yellow stunting dysfunction virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a serious risk to industrial cucurbit manufacturing worldwide. Right here, development of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts in a position to replicate in cucumber protoplasts. CYSDV RNA1 proved competent for replication; transcription of each polarities of the genomic RNA was detectable 24 h submit inoculation.

Hybridization of complete RNA extracted from transfected protoplasts or from naturally CYSDV-infected cucurbits revealed high-level transcription of the p22 subgenomic RNA species. Replication of CYSDV RNA2 following co-transfection with RNA1 was additionally noticed, with comparable transcription kinetics. A CYSDV RNA2 cDNA clone (T3CM8Δ) comprising the 5′- and three’-UTRs plus the three’-terminal gene, generated a 2.eight kb RNA in a position to replicate to excessive ranges in protoplasts within the presence of CYSDV RNA1. The clone T3CM8Δ will facilitate reverse genetics research of CYSDV gene operate and RNA replication determinants.

A single-plasmid reverse genetics system for the rescue of non-segmented negative-strand RNA viruses from cloned full-length cDNA.

Reverse genetics techniques for non-segmented negative-strand RNA viruses depend on co-transfection of a plasmid containing the full-length viral cDNA and helper plasmids encoding important viral replication proteins. Right here, a system is offered wherein virus will be rescued from a single plasmid with out the necessity for helper plasmids in cells contaminated with a host-restricted recombinant poxvirus that expresses T7 RNA polymerase.

Chrna3/ Rat Chrna3 ELISA Kit

ELI-49491r 96 Tests
EUR 1063.2

CHRNA3 Antibody

1-CSB-PA085715
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against CHRNA3. Recognizes CHRNA3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:25-1:100

CHRNA3 Antibody

1-CSB-PA000802
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CHRNA3. Recognizes CHRNA3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000

CHRNA3 siRNA

20-abx911778
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

CHRNA3 siRNA

20-abx911779
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

CHRNA3 Antibody

1-CSB-PA005389GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against CHRNA3. Recognizes CHRNA3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

CHRNA3 antibody

70R-49550 100 ul
EUR 292.8
Description: Purified Polyclonal CHRNA3 antibody

CHRNA3 antibody

70R-5188 50 ug
EUR 560.4
Description: Rabbit polyclonal CHRNA3 antibody raised against the N terminal of CHRNA3

CHRNA3 antibody

70R-10537 50 ug
EUR 560.4
Description: Affinity purified rabbit polyclonal CHRNA3 antibody

CHRNA3 antibody

70R-16408 50 ul
EUR 522
Description: Rabbit polyclonal CHRNA3 antibody

CHRNA3 antibody

20R-1327 100 ug
EUR 452.4
Description: Rabbit polyclonal CHRNA3 antibody

CHRNA3 Antibody

31245-100ul 100ul
EUR 302.4

CHRNA3 Antibody

31245-50ul 50ul
EUR 224.4

CHRNA3 protein

80R-4244 100 ug
EUR 418.8
Description: Recombinant Human CHRNA3 protein with His tag

CHRNA3 Antibody

RQ4415 100 ug
EUR 419

Genorise® Chicken IL-6 cDNA Clone

GR147003 10 µg
EUR 183.6

Genorise® Chicken IL-4 cDNA Clone

GR147004 10 µg
EUR 183.6

Genorise® Chicken IL-10 cDNA Clone

GR147005 10 µg
EUR 183.6

Anti-ASFV P72 Antibody | MASF-5A-Clone #10E11

MASF-5A-Clone-10E11 1 mg
EUR 667
Description:

Anti-ASFV P72 Antibody | MASF-5A-Clone #10E11 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-ASFV P72 Antibody | MASF-5A-Clone #10G5

MASF-5A-Clone-10G5 1 mg
EUR 667
Description:

Anti-ASFV P72 Antibody | MASF-5A-Clone #10G5 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Genorise® Human GLUT1 cDNA Clone, 10 ug

GR154002 10 µg
EUR 183.6

Genorise® Human cTnT cDNA Clone, 10 ug

GR154003 10 µg
EUR 183.6

Genorise® Human TNN3 cDNA Clone, 10 ug

GR154004 10 µg
EUR 183.6

Genorise® Human BNP cDNA Clone, 10 ug

GR154005 10 µg
EUR 183.6

Genorise® Human Myoglobin cDNA Clone, 10 ug

GR154006 10 µg
EUR 183.6

Genorise® Human ANP cDNA Clone, 10 ug

GR154008 10 µg
EUR 183.6

Genorise® Human Myeloperoxidase cDNA Clone, 10 ug

GR154009 10 µg
EUR 183.6

Genorise® Human GPBB cDNA Clone, 10 ug

GR154010 10 µg
EUR 183.6

Genorise® Human CRP cDNA Clone, 10 ug

GR154011 10 µg
EUR 183.6

Genorise® Human MyBPC3 cDNA Clone, 10 ug

GR154012 10 µg
EUR 183.6

CHRNA3 Conjugated Antibody

C31245 100ul
EUR 476.4

CHRNA3 cloning plasmid

CSB-CL005389HU1-10ug 10ug
EUR 451.2
Description: A cloning plasmid for the CHRNA3 gene.

CHRNA3 cloning plasmid

CSB-CL005389HU2-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the CHRNA3 gene.

anti- CHRNA3 antibody

FNab01676 100µg
EUR 658.5
Description: Antibody raised against CHRNA3

CHRNA3 Blocking Peptide

33R-2458 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CHRNA3 antibody, catalog no. 70R-5188

CHRNA3 Blocking Peptide

33R-7520 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CHRNA3 antibody, catalog no. 20R-1327

CHRNA3 Blocking Peptide

20-abx062425
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

CHRNA3 Rabbit pAb

A1674-100ul 100 ul
EUR 369.6

CHRNA3 Rabbit pAb

A1674-200ul 200 ul
EUR 550.8

CHRNA3 Rabbit pAb

A1674-20ul 20 ul
EUR 219.6

CHRNA3 Rabbit pAb

A1674-50ul 50 ul
EUR 267.6

Anti-CHRNA3 antibody

PAab01676 100 ug
EUR 463.2

pOTB7-CHRNA3 Plasmid

PVTB00094S 2 ug
EUR 427.2

Anti-CHRNA3 antibody

STJ111103 100 µl
EUR 332.4
Description: This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#3B11

MALV-30A-Clone-3B11 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#3B11 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#5A4

MALV-30A-Clone-5A4 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#5A4 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6C2

MALV-30A-Clone-6C2 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6C2 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6D7

MALV-30A-Clone-6D7 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6D7 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Genorise® Sheep IL-6 cDNA Clone, 10 ug

GR151001 10 µg
EUR 183.6

Genorise® Ovine IL-2 cDNA Clone, 10 ug

GR151002 10 µg
EUR 183.6

Genorise® Ovine IL-4 cDNA Clone, 10 ug

GR151003 10 µg
EUR 183.6

Genorise® Bovine IL-1b cDNA Clone, 10 ug

GR152001 10 µg
EUR 183.6

Genorise® Rabbit IL-1b cDNA Clone, 10 ug

GR153001 10 µg
EUR 183.6

Genorise® Human H-FABP cDNA Clone, 10 ug

GR154001 10 µg
EUR 183.6

Genorise® Human Lp-PLA2 cDNA Clone, 10 ug

GR154007 10 µg
EUR 183.6

Genorise® Human PAPP-A cDNA Clone, 10 ug

GR154013 10 µg
EUR 183.6

Genorise® Human NT-proBNP cDNA Clone, 10 ug

GR154014 10 µg
EUR 183.6

Genorise® Human Pro-C3 cDNA Clone, 10 ug

GR154015 10 µg
EUR 183.6

Genorise® Canine NGF-B cDNA Clone, 10 ug

GR159001 10 µg
EUR 183.6

Genorise® Canine IL-7 cDNA Clone, 10 ug

GR159002 10 µg
EUR 183.6

Recombinant Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3)

4-RPD143Hu01
  • EUR 442.56
  • EUR 242.40
  • EUR 1329.60
  • EUR 523.20
  • EUR 926.40
  • EUR 372.00
  • EUR 3144.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Cholinergic Receptor, Nicotinic, Alpha 3 expressed in: E.coli

Mouse CHRNA3 shRNA Plasmid

20-abx980106
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Human CHRNA3 shRNA Plasmid

20-abx950813
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Bovine CHRNA3 ELISA KIT

ELI-49438b 96 Tests
EUR 1113.6

Human CHRNA3 ELISA KIT

ELI-49490h 96 Tests
EUR 988.8

Chicken CHRNA3 ELISA KIT

ELI-24546c 96 Tests
EUR 1113.6

Mouse Chrna3 ELISA KIT

ELI-11878m 96 Tests
EUR 1038

CHRNA3 ELISA KIT|Human

EF008656 96 Tests
EUR 826.8

pcDNA3.1(+)-CHRNA3(P229H) Plasmid

PVTB00094-2a 2 ug
EUR 427.2

CHRNA3 Recombinant Protein (Human)

RP007060 100 ug Ask for price

CHRNA3 Recombinant Protein (Human)

RP007063 100 ug Ask for price

CHRNA3 Recombinant Protein (Mouse)

RP124064 100 ug Ask for price

CHRNA3 Recombinant Protein (Rat)

RP194945 100 ug Ask for price

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 678.00
  • EUR 577.20
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Novo? cDNA Kit

M1165-100
EUR 424.8

Novo? cDNA Kit

M1165-25
EUR 320.4

Evo? cDNA Supermix

M1168-100
EUR 457.2

Evo? cDNA Supermix

M1168-25
EUR 320.4

Novo? cDNA Supermix

M1169-100
EUR 529.2

Novo? cDNA Supermix

M1169-25
EUR 346.8

Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit

SED143Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in Tissue homogenates and other biological fluids.

Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit

SED143Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in Tissue homogenates and other biological fluids.

Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit

SED143Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in Tissue homogenates and other biological fluids.

Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit

SED143Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in Tissue homogenates and other biological fluids.

Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit

4-SED143Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

CHRNA3 ELISA Kit (Human) (OKCD00894)

OKCD00894 96 Wells
EUR 997.2
Description: Description of target: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.107 ng/mL

Chrna3 ORF Vector (Rat) (pORF)

ORF064983 1.0 ug DNA
EUR 607.2

CHRNA3 ORF Vector (Human) (pORF)

ORF002354 1.0 ug DNA
EUR 114

CHRNA3 ORF Vector (Human) (pORF)

ORF002355 1.0 ug DNA
EUR 114

Chrna3 ORF Vector (Mouse) (pORF)

ORF041356 1.0 ug DNA
EUR 607.2

pECMV-Chrna3-m-FLAG Plasmid

PVT14993 2 ug
EUR 390

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse)

4-PAD143Hu01
  • EUR 296.40
  • EUR 3012.00
  • EUR 750.00
  • EUR 372.00
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3)

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 744.00
  • EUR 627.60
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 1045.20
  • EUR 794.40
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

CHRNA3 sgRNA CRISPR Lentivector set (Human)

K0448801 3 x 1.0 ug
EUR 406.8

Chrna3 sgRNA CRISPR Lentivector set (Mouse)

K3739501 3 x 1.0 ug
EUR 406.8

Chrna3 sgRNA CRISPR Lentivector set (Rat)

K6844801 3 x 1.0 ug
EUR 406.8

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen

C1236246Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach

C1236248Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human eNOS cDNA probe

eNOS51-D-2 2 ug
EUR 534

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

cDNA Probe Diluent Solution

AR0063 5mL
EUR 127.2

Evo™ cDNA Kit

M1164-100 Ask for price

Evo™ cDNA Kit

M1164-25 Ask for price

Novo? Transcriptome cDNA Kit

M1167-100
EUR 1142.4

Novo? Transcriptome cDNA Kit

M1167-25
EUR 529.2

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 202.8

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 202.8

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 370.8

Plant Tissue cDNA: Arabidopsis

PC34-310 10 rxn
EUR 498

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), APC

4-PAD143Hu01-APC
  • EUR 414.00
  • EUR 3930.00
  • EUR 1094.40
  • EUR 528.00
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with APC.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), Biotinylated

4-PAD143Hu01-Biotin
  • EUR 373.20
  • EUR 2952.00
  • EUR 872.40
  • EUR 457.20
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with Biotin.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), Cy3

4-PAD143Hu01-Cy3
  • EUR 502.80
  • EUR 5190.00
  • EUR 1410.00
  • EUR 654.00
  • EUR 301.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with Cy3.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), FITC

4-PAD143Hu01-FITC
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with FITC.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), HRP

4-PAD143Hu01-HRP
  • EUR 379.20
  • EUR 3426.00
  • EUR 968.40
  • EUR 477.60
  • EUR 247.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with HRP.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), PE

4-PAD143Hu01-PE
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with PE.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Human, Mouse), APC-Cy7

4-PAD143Hu01-APC-Cy7
  • EUR 685.20
  • EUR 7716.00
  • EUR 2046.00
  • EUR 912.00
  • EUR 382.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). This antibody is labeled with APC-Cy7.

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Antibody

20-abx171728
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody

20-abx123004
  • EUR 493.20
  • EUR 710.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 50 ul

Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody

20-abx125675
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Antibody

20-abx130886
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNA3) Antibody

20-abx111610
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody

20-abx009435
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody

abx032382-400ul 400 ul
EUR 627.6

Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody

abx032382-80l 80 µl
EUR 343.2

Cholinergic Receptor, Nicotinic Alpha 3 (CHRNA3) Antibody

abx231676-100ug 100 ug
EUR 610.8

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNA3) Antibody

20-abx327182
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNA3) Antibody

20-abx241009
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

CHRNA3 sgRNA CRISPR Lentivector (Human) (Target 1)

K0448802 1.0 ug DNA
EUR 184.8

CHRNA3 sgRNA CRISPR Lentivector (Human) (Target 2)

K0448803 1.0 ug DNA
EUR 184.8

CHRNA3 sgRNA CRISPR Lentivector (Human) (Target 3)

K0448804 1.0 ug DNA
EUR 184.8

Chrna3 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3739502 1.0 ug DNA
EUR 184.8

Chrna3 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3739503 1.0 ug DNA
EUR 184.8

Chrna3 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3739504 1.0 ug DNA
EUR 184.8

Chrna3 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6844802 1.0 ug DNA
EUR 184.8

Chrna3 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6844803 1.0 ug DNA
EUR 184.8

Chrna3 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6844804 1.0 ug DNA
EUR 184.8

This method depends on the insertion of T7 promoter sequences within the viral cDNA at positions that enable transcription of sub-genomic RNAs encoding important viral replication proteins.

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