Evaluation of gene expression is a standard analysis instrument to check networks controlling gene expression, the position of genes with unknown perform, and environmentally induced responses of organisms. A lot of the analytical instruments used to investigate gene expression depend on correct cDNA synthesis and quantification to acquire reproducible and quantifiable outcomes. So far, most business kits for isolation and purification of cDNA goal double-stranded molecules, which don’t precisely characterize the abundance of transcripts.

Within the current report, we offer a easy and quick technique to purify single-stranded cDNA, exhibiting excessive purity and yield. This technique is predicated on the therapy with RNase H and RNase A after cDNA synthesis, adopted by separation in silica spin-columns and ethanol precipitation. As well as, our technique avoids the usage of DNase I to get rid of genomic DNA from RNA preparations, which improves cDNA yield. As a case report, our technique proved to be helpful within the purification of single-stranded cDNA from the pathogenic fungus Sporothrix schenckii.

Destiny of HIV-1 cDNA intermediates throughout reverse transcription is dictated by transcription initiation web site of virus genomic RNA.

Retroviral reverse transcription is completed by sequential strand-transfers of partial cDNA intermediates copied from viral genomic RNA. Right here, we revealed an unprecedented position of 5′-end guanosine (G) of HIV-1 genomic RNA for reverse transcription. Based mostly on present consensus for HIV-1 transcription initiation web site, HIV-1 transcripts possess a single G at 5′-ends (G1-form). Nonetheless, we discovered that HIV-1 transcripts with further Gs at 5′-ends (G2- and G3-forms) had been abundantly expressed in contaminated cells by utilizing various transcription initiation websites.

The G2- and G3-forms had been additionally detected within the virus particle, though the G1-form predominated. To handle organic affect of the 5′-G quantity, we generated HIV clone DNA to precise the G1-form solely by deleting the choice initiation websites. Virus produced from the clone confirmed considerably larger strand-transfer of minus strong-stop cDNA (-sscDNA). The in vitro assay utilizing artificial HIV-1 RNAs revealed that the abortive types of -sscDNA had been abundantly generated from the G3-form RNA, however dramatically diminished from the G1-form. Furthermore, the strand-transfer of -sscDNA from the G1-form was prominently stimulated by HIV-1 nucleocapsid. Taken collectively, our outcomes demonstrated that the 5′-G quantity that corresponds to HIV-1 transcription initiation web site was vital for profitable strand-transfer of -sscDNA throughout reverse transcription.

Extraction of Complete DNA and RNA from Marine Filter Samples and Era of a cDNA as Common Template for Marker Gene Research.

Microbial communities play an vital position in marine ecosystem processes. Though the variety of research concentrating on marker genes such because the 16S rRNA gene has been elevated in the previous few years, the overwhelming majority of marine range is relatively unexplored.

Furthermore, most research centered on the complete bacterial group and thus disregarded energetic microbial group gamers. Right here, we describe an in depth protocol for the simultaneous extraction of DNA and RNA from marine water samples and for the technology of cDNA from the remoted RNA which can be utilized as a common template in numerous marker gene research.

Probe-Directed Degradation (PDD) for Versatile Removing of Undesirable cDNA Sequences from RNA-Seq Libraries.

Most purposes for RNA-seq require the depletion of plentiful transcripts to realize higher protection of the underlying transcriptome. The sequences to be focused for depletion rely upon software and species and in lots of circumstances will not be supported by business depletion kits. This unit describes a way for producing RNA-seq libraries that includes probe-directed degradation (PDD), which might deplete any undesirable sequence set, with the low-bias split-adapter technique of library technology (though many different library technology strategies are in precept suitable).

The general technique is appropriate for purposes requiring personalized sequence depletion or the place trustworthy illustration of fragment ends and lack of sequence bias is paramount. We offer tips to quickly design particular probes towards the goal sequence, and an in depth protocol for library technology utilizing the split-adapter technique together with a number of methods for streamlining the approach and lowering adapter dimer content material.

Defects in RNase H2 Stimulate DNA Break Restore by RNA Reverse Transcribed into cDNA.

Eukaryotic ribonucleases (RNase) H1 and H2 are endonucleases that cleave RNA in a double- stranded RNA-DNA molecule. RNase H2 may cleave a single ribonucleotide embedded in DNA duplex. Whereas the exercise of RNase H1 and H2 has been extensively characterised in vitro, nonetheless a lot is unclear concerning the particular targets of those enzymes in vivo. We not too long ago demonstrated that yeast cells can restore a double-strand break (DSB) in DNA by homologous recombination (HR) utilizing antisense (non-coding) RNA, both straight, or not directly after changing RNA into cDNA.

In wildtype RNase H1 and/or H2 cells, restore by cDNA dominates, whereas within the absence of RNase H1 and H2 capabilities cDNA and, particularly, direct transcript-RNA restore mechanisms are markedly stimulated. Right here we discovered that null alleles of any of the three RNase H2 subunits stimulate DSB restore by cDNA considerably greater than a null allele of RNase H1.

These outcomes present that RNase H2 is the popular RNase H enzyme to focus on cDNA in yeast. Concentrating on of cDNA by RNase H2 doesn’t require RNase H2 interplay with the DNA clamp proliferating cell nuclear antigen (PCNA).

Genorise® Human cTnT cDNA Clone, 10 ug
GR154003	Genorise Scientific	10 µg	EUR 153

Genorise® Human TNN3 cDNA Clone, 10 ug
GR154004	Genorise Scientific	10 µg	EUR 153

Genorise® Human GPBB cDNA Clone, 10 ug
GR154010	Genorise Scientific	10 µg	EUR 153

Genorise® Human GLUT1 cDNA Clone, 10 ug
GR154002	Genorise Scientific	10 µg	EUR 153

Genorise® Human MyBPC3 cDNA Clone, 10 ug
GR154012	Genorise Scientific	10 µg	EUR 153

Genorise® Human Myoglobin cDNA Clone, 10 ug
GR154006	Genorise Scientific	10 µg	EUR 153

Genorise® Sheep IL-6 cDNA Clone, 10 ug
GR151001	Genorise Scientific	10 µg	EUR 153

Genorise® Ovine IL-2 cDNA Clone, 10 ug
GR151002	Genorise Scientific	10 µg	EUR 153

Genorise® Ovine IL-4 cDNA Clone, 10 ug
GR151003	Genorise Scientific	10 µg	EUR 153

Genorise® Canine IL-7 cDNA Clone, 10 ug
GR159002	Genorise Scientific	10 µg	EUR 153

Genorise® Bovine IL-1b cDNA Clone, 10 ug
GR152001	Genorise Scientific	10 µg	EUR 153

Genorise® Rabbit IL-1b cDNA Clone, 10 ug
GR153001	Genorise Scientific	10 µg	EUR 153

Genorise® Human H-FABP cDNA Clone, 10 ug
GR154001	Genorise Scientific	10 µg	EUR 153

Genorise® Human PAPP-A cDNA Clone, 10 ug
GR154013	Genorise Scientific	10 µg	EUR 153

Genorise® Human Pro-C3 cDNA Clone, 10 ug
GR154015	Genorise Scientific	10 µg	EUR 153

Genorise® Canine NGF-B cDNA Clone, 10 ug
GR159001	Genorise Scientific	10 µg	EUR 153

Genorise® Human Lp-PLA2 cDNA Clone, 10 ug
GR154007	Genorise Scientific	10 µg	EUR 153

Genorise® Human Myeloperoxidase cDNA Clone, 10 ug
GR154009	Genorise Scientific	10 µg	EUR 153

Genorise® Human NT-proBNP cDNA Clone, 10 ug
GR154014	Genorise Scientific	10 µg	EUR 153

Human Neuroplastin (NPTN) ELISA Kit
SEC666Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neuroplastin (NPTN) in tissue homogenates, cell lysates and other biological fluids.

Human Neuroplastin (NPTN) ELISA Kit
SEC666Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neuroplastin (NPTN) in tissue homogenates, cell lysates and other biological fluids.

Human Neuroplastin (NPTN) ELISA Kit
SEC666Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neuroplastin (NPTN) in tissue homogenates, cell lysates and other biological fluids.

Human Neuroplastin (NPTN) ELISA Kit
SEC666Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neuroplastin (NPTN) in tissue homogenates, cell lysates and other biological fluids.

Human Neuroplastin (NPTN) ELISA Kit
4-SEC666Hu	Cloud-Clone	EUR 5738.40 EUR 3031.20 EUR 768.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Neuroplastin (NPTN) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

NPTN cloning plasmid
CSB-CL016028HU-10ug	Cusabio	10ug	EUR 279.6
Description: A cloning plasmid for the NPTN gene.

NPTN siRNA
20-abx903630	Abbexa	EUR 661.20 EUR 878.40	15 nmol 30 nmol

NPTN siRNA
20-abx926287	Abbexa	EUR 661.20 EUR 878.40	15 nmol 30 nmol

NPTN siRNA
20-abx926288	Abbexa	EUR 661.20 EUR 878.40	15 nmol 30 nmol

NPTN antibody
10R-7067	Fitzgerald	100 ul	EUR 871.2
Description: Mouse monoclonal NPTN antibody

NPTN antibody
10R-7068	Fitzgerald	100 ul	EUR 829.2
Description: Mouse monoclonal NPTN antibody

NPTN antibody
10R-7069	Fitzgerald	100 ul	EUR 829.2
Description: Mouse monoclonal NPTN antibody

NPTN Antibody
1-CSB-PA008837	Cusabio	EUR 266.40 EUR 234.00	100ug 50ug
Description: A polyclonal antibody against NPTN. Recognizes NPTN from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/5000

NPTN Antibody
1-CSB-PA016028ESR1HU	Cusabio	EUR 266.40 EUR 402.00	100ul 50ul
Description: A polyclonal antibody against NPTN. Recognizes NPTN from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

NPTN Antibody
1-CSB-PA016028ESR2HU	Cusabio	EUR 266.40 EUR 402.00	100ul 50ul
Description: A polyclonal antibody against NPTN. Recognizes NPTN from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

NPTN Antibody
DF13829	Affinity Biosciences	100ul	EUR 280
Description: Human,Mouse,Rat

NPTN Antibody
E308476	EnoGene	200ul	EUR 275
Description: Available in various conjugation types.

NPTN Antibody
E11-17029C	EnoGene	100μg	EUR 225
Description: Available in various conjugation types.

NPTN Antibody
DF13829-100ul	Affinity Biosciences	100ul	EUR 280

NPTN Antibody
DF13829-200ul	Affinity Biosciences	200ul	EUR 350

NPTN Antibody
CAC10808-100ul	Biomatik Corporation	100ul	EUR 314

NPTN Antibody
CAC10808-50ul	Biomatik Corporation	50ul	EUR 199.2

NPTN Rabbit pAb
A7972-100ul	Abclonal	100 ul	EUR 369.6

NPTN Rabbit pAb
A7972-200ul	Abclonal	200 ul	EUR 550.8

NPTN Rabbit pAb
A7972-20ul	Abclonal	20 ul	EUR 219.6

NPTN Rabbit pAb
A7972-50ul	Abclonal	50 ul	EUR 267.6

pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]
CD510VB-1	SBI	>1 x 10^6 IFUs	EUR 720

pCDF1-MCS1 cDNA Cloning and Expression Vector
CD100A-1	SBI	10 ug	EUR 483

NPTN Polyclonal Antibody
31395-100ul	SAB	100ul	EUR 302.4

NPTN Polyclonal Antibody
31395-50ul	SAB	50ul	EUR 224.4

NPTN Polyclonal Antibody
E97972	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

Neuroplastin (NPTN) Antibody
20-abx173801	Abbexa	EUR 1028.40 EUR 526.80	1 mg 200 ug

Neuroplastin (NPTN) Antibody
20-abx177788	Abbexa	EUR 1446.00 EUR 693.60	1 mg 200 ug

Neuroplastin (NPTN) Antibody
abx146042-100ug	Abbexa	100 ug	EUR 469.2

Neuroplastin (NPTN) Antibody
20-abx321948	Abbexa	EUR 526.80 EUR 393.60	100 ul 50 ul

Neuroplastin (NPTN) Antibody
20-abx326686	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Neuroplastin (NPTN) Antibody
abx027055-400ul	Abbexa	400 ul	EUR 627.6

Neuroplastin (NPTN) Antibody
abx027055-80l	Abbexa	80 µl	EUR 343.2

Neuroplastin (NPTN) Antibody
20-abx321072	Abbexa	EUR 526.80 EUR 393.60	100 ul 50 ul

Neuroplastin (NPTN) Antibody
20-abx006050	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

NPTN ELISA KIT|Human
EF007364	Lifescience Market	96 Tests	EUR 826.8

pCDH-CMV-MCS cDNA Cloning and Expression Vector
CD500B-1	SBI	10 ug	EUR 483

pCDH-EF1-MCS cDNA Cloning and Expression Vector
CD502A-1	SBI	10 ug	EUR 483

pCDH-CMV-MCS2 cDNA Cloning and Expression Vector
CD501A-1	SBI	10 ug	EUR 483

Rat NPTN shRNA Plasmid
20-abx985858	Abbexa	EUR 961.20 EUR 1345.20	150 µg 300 µg

Human NPTN shRNA Plasmid
20-abx958925	Abbexa	EUR 961.20 EUR 1345.20	150 µg 300 µg

Mouse NPTN shRNA Plasmid
20-abx972619	Abbexa	EUR 961.20 EUR 1345.20	150 µg 300 µg

cDNA - Plant Normal Tissue: cDNA - Plant: Corn
C1634330	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Rice
C1634360	Biochain	40 reactions	EUR 674

NPTN Recombinant Protein (Rat)
RP214388	ABM	100 ug	Ask for price

cDNA - Plant Normal Tissue: cDNA - Plant: Wheat
C1634390	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Orange
C1634340	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Potato
C1634350	Biochain	40 reactions	EUR 674

NPTN Recombinant Protein (Mouse)
RP154784	ABM	100 ug	Ask for price

NPTN Recombinant Protein (Human)
RP041734	ABM	100 ug	Ask for price

Human Neuroplastin (NPTN) Protein
20-abx654543	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis
C1634310	Biochain	40 reactions	EUR 674

Nptn ORF Vector (Rat) (pORF)
ORF071464	ABM	1.0 ug DNA	EUR 607.2

pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector
CD111B-1	SBI	10 ug	EUR 572

NPTN Polyclonal Conjugated Antibody
C31395	SAB	100ul	EUR 476.4

NPTN ORF Vector (Human) (pORF)
ORF013912	ABM	1.0 ug DNA	EUR 424.8

Nptn ORF Vector (Mouse) (pORF)
ORF051596	ABM	1.0 ug DNA	EUR 607.2

PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector
PB511B-1	SBI	10 ug	EUR 638

PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector
PB512B-1	SBI	10 ug	EUR 638

cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean
C1634370	Biochain	40 reactions	EUR 674

pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector
CD110B-1	SBI	10 ug	EUR 572

PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector
PB510B-1	SBI	10 ug	EUR 638

PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector
PB530A-2	SBI	10 ug	EUR 638

PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
PB531A-2	SBI	10 ug	EUR 638

PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector
PB533A-2	SBI	10 ug	EUR 638

pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector
CD512B-1	SBI	10 ug	EUR 572

pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector
CD514B-1	SBI	10 ug	EUR 572

pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector
CD510B-1	SBI	10 ug	EUR 572

pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector
CD530A-2	SBI	10 ug	EUR 590

pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
CD531A-2	SBI	10 ug	EUR 590

pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector
CD533A-2	SBI	10 ug	EUR 590

pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector
CD630A-1	SBI	10 ug	EUR 590

pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector
CD711B-1	SBI	10 ug	EUR 572

pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector
CD515B-1	SBI	10 ug	EUR 572

pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector
CD522A-1	SBI	10 ug	EUR 590

pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector
CD615B-1	SBI	10 ug	EUR 572

pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector
CD532A-2	SBI	10 ug	EUR 590

pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector
CD710B-1	SBI	10 ug	EUR 572

pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector
CD731B-1	SBI	10 ug	EUR 590

PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector
PB514B-2	SBI	10 ug	EUR 638

NPTN ELISA Kit (Human) (OKCD08220)
OKCD08220	Aviva Systems Biology	96 Wells	EUR 1170
Description: Description of target: Neuroplastin is a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. The alpha and beta transcripts show differential localization within the brain.Neuroplastin is a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. The alpha and beta transcripts show differential localization within the brain.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.058ng/mL

NPTN ELISA Kit (Human) (OKDD00432)
OKDD00432	Aviva Systems Biology	96 Wells	EUR 1170
Description: Description of target: This gene encodes a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. Alternative splicing results in multiple transcript variants.;Species reactivity: Human;Application: ;Assay info: Quantitative Sandwich ELISA;Sensitivity: < 0.052 ng/mL

Human NPTN Protein Lysate 20ug
IHUNPTNPLLY20UG	Innovative research	each	EUR 213
Description: Human NPTN Protein Lysate 20ug

pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector
CD511B-1	SBI	10 ug	EUR 572

pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector
CD524A-1	SBI	10 ug	EUR 590

Rat Neuroplastin (NPTN) ELISA Kit
abx391703-96tests	Abbexa	96 tests	EUR 1093.2

Rat Neuroplastin, Nptn ELISA KIT
ELI-35323r	Lifescience Market	96 Tests	EUR 1063.2

PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector
PB513B-1	SBI	10 ug	EUR 638

pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged]
CD512VA-1	SBI	>1 x 10^6 IFUs	EUR 720

Human Neuroplastin (NPTN) CLIA Kit
20-abx493824	Abbexa	EUR 9567.60 EUR 5095.20 EUR 1177.20	10 × 96 tests 5 × 96 tests 96 tests

PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
PB713B-1	SBI	10 ug	EUR 638

Human Neuroplastin (NPTN) ELISA Kit
20-abx152508	Abbexa	EUR 8853.60 EUR 4719.60 EUR 1093.20	10 × 96 tests 5 × 96 tests 96 tests

Human Neuroplastin (NPTN) ELISA Kit
abx257574-96tests	Abbexa	96 tests	EUR 764.4

Mouse Neuroplastin (NPTN) ELISA Kit
abx390037-96tests	Abbexa	96 tests	EUR 1093.2

Human Neuroplastin (NPTN) ELISA Kit
DLR-NPTN-Hu-48T	DL Develop	48T	EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Neuroplastin (NPTN) in samples from tissue homogenates or other biological fluids.

Human Neuroplastin (NPTN) ELISA Kit
DLR-NPTN-Hu-96T	DL Develop	96T	EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Neuroplastin (NPTN) in samples from tissue homogenates or other biological fluids.

Human NPTN(Neuroplastin) ELISA Kit
EH4175	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Mouse Neuroplastin, Nptn ELISA KIT
ELI-39707m	Lifescience Market	96 Tests	EUR 1038

Human Neuroplastin, NPTN ELISA KIT
ELI-14849h	Lifescience Market	96 Tests	EUR 988.8

Human Neuroplastin (NPTN) ELISA Kit
RD-NPTN-Hu-48Tests	Reddot Biotech	48 Tests	EUR 625.2

Human Neuroplastin (NPTN) ELISA Kit
RD-NPTN-Hu-96Tests	Reddot Biotech	96 Tests	EUR 867.6

Human Neuroplastin (NPTN) ELISA Kit
RDR-NPTN-Hu-48Tests	Reddot Biotech	48 Tests	EUR 652.8

Human Neuroplastin (NPTN) ELISA Kit
RDR-NPTN-Hu-96Tests	Reddot Biotech	96 Tests	EUR 907.2

Human Neuroplastin (NPTN) ELISA Kit
RDR-NPTN-Hu-48T	Reddot Biotech	48T	EUR 475.34
Description: tissue homogenates and other biological fluids.

Human Neuroplastin (NPTN) ELISA Kit
RDR-NPTN-Hu-96T	Reddot Biotech	96T	EUR 679.04
Description: tissue homogenates and other biological fluids.

Human Neuroplastin (NPTN) ELISA Kit
RD-NPTN-Hu-48T	Reddot Biotech	48T	EUR 452.7
Description: tissue homogenates and other biological fluids.

Human Neuroplastin (NPTN) ELISA Kit
RD-NPTN-Hu-96T	Reddot Biotech	96T	EUR 646.7
Description: tissue homogenates and other biological fluids.

Human Neuroplastin(NPTN)ELISA Kit
QY-E04762	Qayee Biotechnology	96T	EUR 433.2

Human Neuroplastin (NPTN) ELISA Kit
DL-NPTN-Hu	DL Develop	96T	EUR 441
Description: tissue homogenates or other biological fluids.

Human Neuroplastin (NPTN) ELISA Kit
DLR-NPTN-Hu	DL Develop	96T	EUR 463
Description: tissue homogenates or other biological fluids.

Human Neuroplastin (NPTN) ELISA Kit
EKN47374-48T	Biomatik Corporation	48T	EUR 378.7

Human Neuroplastin (NPTN) ELISA Kit
EKN47374-5x96T	Biomatik Corporation	5x96T	EUR 2569.75

Human Neuroplastin (NPTN) ELISA Kit
EKN47374-96T	Biomatik Corporation	96T	EUR 541

Human Neuroplastin (NPTN) ELISA Kit
EKU06263-48T	Biomatik Corporation	48T	EUR 555.66

Human Neuroplastin (NPTN) ELISA Kit
EKU06263-5x96T	Biomatik Corporation	5x96T	EUR 3770.55

Human Neuroplastin (NPTN) ELISA Kit
EKU06263-96T	Biomatik Corporation	96T	EUR 793.8

Human NPTN(Neuroplastin) ELISA Kit
ELK4704-48T	ELK Biotech	48T	Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NPTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NPTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPTN in the samples is then determined by comparing the OD of the samples to the standard curve.

Human NPTN(Neuroplastin) ELISA Kit
ELK4704-96T	ELK Biotech	96T	Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NPTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NPTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPTN in the samples is then determined by comparing the OD of the samples to the standard curve.

pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
CD513B-1	SBI	10 ug	EUR 572

Novo? cDNA Kit
M1165-100	Biovision	each	EUR 424.8

Novo? cDNA Kit
M1165-25	Biovision	each	EUR 320.4

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector
CD516B-2	SBI	10 ug	EUR 572

pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector
CD713B-1	SBI	10 ug	EUR 572

cDNA - Lupus: Lung
C1236152Lup	Biochain	40 reactions	EUR 737

cDNA - Lupus: Colon
C1236090Lup	Biochain	40 reactions	EUR 737

cDNA - Lupus: Heart
C1236122Lup	Biochain	40 reactions	EUR 737

cDNA - Lupus: Liver
C1236149Lup	Biochain	40 reactions	EUR 737

cDNA - Asthma: Lung
C1236152Ld-1	Biochain	40 reactions	EUR 899

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged]
CD516VB-2	SBI	>1 x 10^6 IFUs	EUR 706

h NPTN inducible lentiviral particles
LVP406	GenTarget	1x107 IFU/ml x 200ul	EUR 541.2
Description: Pre-made inducible lentiviral particles for expressing human target: NPTN (alternative name: GP55; GP65; SDR1; np55; np65; SDFR1), with ORF sequence 100% matching to CDS region in NCBI ID: NM_017455.

cDNA - Lupus: Kidney
C1236142Lup	Biochain	40 reactions	EUR 737

cDNA - Lupus: Spleen
C1236246Lup	Biochain	40 reactions	EUR 737

Evo? cDNA Supermix
M1168-100	Biovision	each	EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision	each	EUR 320.4

pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg
CD527A-1	SBI	10 ug	EUR 590

cDNA - Lupus: stomach
C1236248Lup	Biochain	40 reactions	EUR 737

Novo? cDNA Supermix
M1169-100	Biovision	each	EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision	each	EUR 346.8

cDNA - Emphysema: Lung
C1236152Ld-3	Biochain	40 reactions	EUR 899

cDNA - Pneumonia: Lung
C1236152Ld-4	Biochain	40 reactions	EUR 899

cDNA - Lupus: Pancreas
C1236188Lup	Biochain	40 reactions	EUR 737

pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged]
CD513VB-1	SBI	>1 x 10^6 IFUs	EUR 720

cDNA - Bronchitis: Lung
C1236152Ld-2	Biochain	40 reactions	EUR 899

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus]
CD511VB-1	SBI	>1 x 10^6 IFUs	EUR 720

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems	Abbexa	EUR 744.00 EUR 627.60	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)
20-abx09802120ulSystems	Abbexa	EUR 1045.20 EUR 794.40	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

cDNA - hypertension: Vein
C1236020Hd-2	Biochain	40 reactions	EUR 729

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	EUR 678.00 EUR 577.20	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

cDNA - hypertension: Heart
C1236122Hd-2	Biochain	40 reactions	EUR 729

Evo™ cDNA Kit
M1164-100	Biovision	each	Ask for price

Evo™ cDNA Kit
M1164-25	Biovision	each	Ask for price

ELISA kit for Human NPTN (Neuroplastin)
ELK4704	ELK Biotech	1 plate of 96 wells	EUR 518.4
Description: A sandwich ELISA kit for detection of Neuroplastin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Nptn sgRNA CRISPR Lentivector set (Rat)
K6791401	ABM	3 x 1.0 ug	EUR 406.8

cDNA - hypertension: Artery
C1236013Hd-2	Biochain	40 reactions	EUR 729

cDNA - hypertension: Kidney
C1236142Hd-2	Biochain	40 reactions	EUR 729

Nptn 3'UTR GFP Stable Cell Line
TU264142	ABM	1.0 ml	Ask for price

Nptn 3'UTR GFP Stable Cell Line
TU164271	ABM	1.0 ml	Ask for price

NPTN 3'UTR GFP Stable Cell Line
TU065917	ABM	1.0 ml	EUR 1672.8

Nptn sgRNA CRISPR Lentivector set (Mouse)
K4866601	ABM	3 x 1.0 ug	EUR 406.8

NPTN sgRNA CRISPR Lentivector set (Human)
K1449901	ABM	3 x 1.0 ug	EUR 406.8

cDNA - Arteriosclerosis: Aorta
C1236012Hd-4	Biochain	40 reactions	EUR 899

cDNA - Arteriosclerosis: Artery
C1236013Hd-4	Biochain	40 reactions	EUR 899

NPTN Protein Vector (Rat) (pPM-C-HA)
PV285856	ABM	500 ng	EUR 723.6

pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector
CD550A-1	SBI	10 ug	EUR 563

Human eNOS cDNA probe
eNOS51-D-2	Alpha Diagnostics	2 ug	EUR 534

NPTN Protein Vector (Rat) (pPB-C-His)
PV285854	ABM	500 ng	EUR 723.6

NPTN Protein Vector (Rat) (pPB-N-His)
PV285855	ABM	500 ng	EUR 723.6

NPTN Protein Vector (Rat) (pPM-C-His)
PV285857	ABM	500 ng	EUR 723.6

Neuroplastin (NPTN) Magnetic Luminex Assay Kit
LKU606111-96T	Biomatik Corporation	96T	EUR 966

cDNA - Lupus: Spinal Cord
C1236234Lup	Biochain	40 reactions	EUR 737

NPTN Protein Vector (Human) (pPM-C-HA)
PV055647	ABM	500 ng	EUR 577.2

NPTN Protein Vector (Mouse) (pPM-C-HA)
PV206384	ABM	500 ng	EUR 723.6

Furthermore, yeast RNase H2 orthologous mutants of two widespread RNase H2 defects related to Aicardi Goutières syndrome (AGS) in people, displayed elevated cDNA-driven restore of a DSB when mixed with one another or with RNase H1 null mutation. Our findings assist the speculation that faulty RNase H2 alleles have larger stage of cDNA derived from both coding or non-coding RNA within the type of RNA-cDNA hybrids.