Evaluation of gene expression is a standard analysis instrument to check networks controlling gene expression, the position of genes with unknown perform, and environmentally induced responses of organisms. A lot of the analytical instruments used to investigate gene expression depend on correct cDNA synthesis and quantification to acquire reproducible and quantifiable outcomes. So far, most business kits for isolation and purification of cDNA goal double-stranded molecules, which don’t precisely characterize the abundance of transcripts.
Within the current report, we offer a easy and quick technique to purify single-stranded cDNA, exhibiting excessive purity and yield. This technique is predicated on the therapy with RNase H and RNase A after cDNA synthesis, adopted by separation in silica spin-columns and ethanol precipitation. As well as, our technique avoids the usage of DNase I to get rid of genomic DNA from RNA preparations, which improves cDNA yield. As a case report, our technique proved to be helpful within the purification of single-stranded cDNA from the pathogenic fungus Sporothrix schenckii.
Destiny of HIV-1 cDNA intermediates throughout reverse transcription is dictated by transcription initiation web site of virus genomic RNA.
Retroviral reverse transcription is completed by sequential strand-transfers of partial cDNA intermediates copied from viral genomic RNA. Right here, we revealed an unprecedented position of 5′-end guanosine (G) of HIV-1 genomic RNA for reverse transcription. Based mostly on present consensus for HIV-1 transcription initiation web site, HIV-1 transcripts possess a single G at 5′-ends (G1-form). Nonetheless, we discovered that HIV-1 transcripts with further Gs at 5′-ends (G2- and G3-forms) had been abundantly expressed in contaminated cells by utilizing various transcription initiation websites.
The G2- and G3-forms had been additionally detected within the virus particle, though the G1-form predominated. To handle organic affect of the 5′-G quantity, we generated HIV clone DNA to precise the G1-form solely by deleting the choice initiation websites. Virus produced from the clone confirmed considerably larger strand-transfer of minus strong-stop cDNA (-sscDNA). The in vitro assay utilizing artificial HIV-1 RNAs revealed that the abortive types of -sscDNA had been abundantly generated from the G3-form RNA, however dramatically diminished from the G1-form. Furthermore, the strand-transfer of -sscDNA from the G1-form was prominently stimulated by HIV-1 nucleocapsid. Taken collectively, our outcomes demonstrated that the 5′-G quantity that corresponds to HIV-1 transcription initiation web site was vital for profitable strand-transfer of -sscDNA throughout reverse transcription.
Extraction of Complete DNA and RNA from Marine Filter Samples and Era of a cDNA as Common Template for Marker Gene Research.
Microbial communities play an vital position in marine ecosystem processes. Though the variety of research concentrating on marker genes such because the 16S rRNA gene has been elevated in the previous few years, the overwhelming majority of marine range is relatively unexplored.

Furthermore, most research centered on the complete bacterial group and thus disregarded energetic microbial group gamers. Right here, we describe an in depth protocol for the simultaneous extraction of DNA and RNA from marine water samples and for the technology of cDNA from the remoted RNA which can be utilized as a common template in numerous marker gene research.
Probe-Directed Degradation (PDD) for Versatile Removing of Undesirable cDNA Sequences from RNA-Seq Libraries.
Most purposes for RNA-seq require the depletion of plentiful transcripts to realize higher protection of the underlying transcriptome. The sequences to be focused for depletion rely upon software and species and in lots of circumstances will not be supported by business depletion kits. This unit describes a way for producing RNA-seq libraries that includes probe-directed degradation (PDD), which might deplete any undesirable sequence set, with the low-bias split-adapter technique of library technology (though many different library technology strategies are in precept suitable).
The general technique is appropriate for purposes requiring personalized sequence depletion or the place trustworthy illustration of fragment ends and lack of sequence bias is paramount. We offer tips to quickly design particular probes towards the goal sequence, and an in depth protocol for library technology utilizing the split-adapter technique together with a number of methods for streamlining the approach and lowering adapter dimer content material.
Defects in RNase H2 Stimulate DNA Break Restore by RNA Reverse Transcribed into cDNA.
Eukaryotic ribonucleases (RNase) H1 and H2 are endonucleases that cleave RNA in a double- stranded RNA-DNA molecule. RNase H2 may cleave a single ribonucleotide embedded in DNA duplex. Whereas the exercise of RNase H1 and H2 has been extensively characterised in vitro, nonetheless a lot is unclear concerning the particular targets of those enzymes in vivo. We not too long ago demonstrated that yeast cells can restore a double-strand break (DSB) in DNA by homologous recombination (HR) utilizing antisense (non-coding) RNA, both straight, or not directly after changing RNA into cDNA.
In wildtype RNase H1 and/or H2 cells, restore by cDNA dominates, whereas within the absence of RNase H1 and H2 capabilities cDNA and, particularly, direct transcript-RNA restore mechanisms are markedly stimulated. Right here we discovered that null alleles of any of the three RNase H2 subunits stimulate DSB restore by cDNA considerably greater than a null allele of RNase H1.
These outcomes present that RNase H2 is the popular RNase H enzyme to focus on cDNA in yeast. Concentrating on of cDNA by RNase H2 doesn’t require RNase H2 interplay with the DNA clamp proliferating cell nuclear antigen (PCNA).
Human Neuroplastin (NPTN) ELISA Kit |
SEC666Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5677.8 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neuroplastin (NPTN) in tissue homogenates, cell lysates and other biological fluids. |
Human Neuroplastin (NPTN) ELISA Kit |
SEC666Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 572.76 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neuroplastin (NPTN) in tissue homogenates, cell lysates and other biological fluids. |
Human Neuroplastin (NPTN) ELISA Kit |
SEC666Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 766.8 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neuroplastin (NPTN) in tissue homogenates, cell lysates and other biological fluids. |
Human Neuroplastin (NPTN) ELISA Kit |
SEC666Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3090.6 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neuroplastin (NPTN) in tissue homogenates, cell lysates and other biological fluids. |
Human Neuroplastin (NPTN) ELISA Kit |
4-SEC666Hu |
Cloud-Clone |
-
EUR 5738.40
-
EUR 3031.20
-
EUR 768.00
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Neuroplastin (NPTN) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. |
NPTN cloning plasmid |
CSB-CL016028HU-10ug |
Cusabio |
10ug |
EUR 279.6 |
|
Description: A cloning plasmid for the NPTN gene. |
NPTN siRNA |
20-abx903630 |
Abbexa |
|
|
|
NPTN siRNA |
20-abx926287 |
Abbexa |
|
|
|
NPTN siRNA |
20-abx926288 |
Abbexa |
|
|
|
NPTN antibody |
10R-7067 |
Fitzgerald |
100 ul |
EUR 871.2 |
Description: Mouse monoclonal NPTN antibody |
NPTN antibody |
10R-7068 |
Fitzgerald |
100 ul |
EUR 829.2 |
Description: Mouse monoclonal NPTN antibody |
NPTN antibody |
10R-7069 |
Fitzgerald |
100 ul |
EUR 829.2 |
Description: Mouse monoclonal NPTN antibody |
NPTN Antibody |
1-CSB-PA008837 |
Cusabio |
|
|
|
Description: A polyclonal antibody against NPTN. Recognizes NPTN from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/5000 |
NPTN Antibody |
1-CSB-PA016028ESR1HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against NPTN. Recognizes NPTN from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
NPTN Antibody |
1-CSB-PA016028ESR2HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against NPTN. Recognizes NPTN from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
NPTN Antibody |
E308476 |
EnoGene |
200ul |
EUR 275 |
Description: Available in various conjugation types. |
NPTN Antibody |
E11-17029C |
EnoGene |
100μg |
EUR 225 |
Description: Available in various conjugation types. |
NPTN Rabbit pAb |
A7972-100ul |
Abclonal |
100 ul |
EUR 369.6 |
NPTN Rabbit pAb |
A7972-200ul |
Abclonal |
200 ul |
EUR 550.8 |
NPTN Rabbit pAb |
A7972-20ul |
Abclonal |
20 ul |
EUR 219.6 |
NPTN Rabbit pAb |
A7972-50ul |
Abclonal |
50 ul |
EUR 267.6 |
pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus] |
CD510VB-1 |
SBI |
>1 x 10^6 IFUs |
EUR 720 |
pCDF1-MCS1 cDNA Cloning and Expression Vector |
CD100A-1 |
SBI |
10 ug |
EUR 483 |
NPTN Polyclonal Antibody |
31395-100ul |
SAB |
100ul |
EUR 302.4 |
NPTN Polyclonal Antibody |
31395-50ul |
SAB |
50ul |
EUR 224.4 |
NPTN Polyclonal Antibody |
E97972 |
EnoGene |
100ul |
EUR 225 |
Description: Available in various conjugation types. |
Neuroplastin (NPTN) Antibody |
20-abx173801 |
Abbexa |
|
|
|
Neuroplastin (NPTN) Antibody |
20-abx177788 |
Abbexa |
|
|
|
Neuroplastin (NPTN) Antibody |
abx146042-100ug |
Abbexa |
100 ug |
EUR 469.2 |
|
Neuroplastin (NPTN) Antibody |
20-abx321948 |
Abbexa |
|
|
|
Neuroplastin (NPTN) Antibody |
20-abx326686 |
Abbexa |
|
|
|
Neuroplastin (NPTN) Antibody |
abx027055-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Neuroplastin (NPTN) Antibody |
abx027055-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Neuroplastin (NPTN) Antibody |
20-abx321072 |
Abbexa |
|
|
|
Neuroplastin (NPTN) Antibody |
20-abx006050 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 218.40
-
EUR 376.80
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
pCDH-CMV-MCS cDNA Cloning and Expression Vector |
CD500B-1 |
SBI |
10 ug |
EUR 483 |
pCDH-EF1-MCS cDNA Cloning and Expression Vector |
CD502A-1 |
SBI |
10 ug |
EUR 483 |
pCDH-CMV-MCS2 cDNA Cloning and Expression Vector |
CD501A-1 |
SBI |
10 ug |
EUR 483 |
Rat NPTN shRNA Plasmid |
20-abx985858 |
Abbexa |
|
|
|
Human NPTN shRNA Plasmid |
20-abx958925 |
Abbexa |
|
|
|
Mouse NPTN shRNA Plasmid |
20-abx972619 |
Abbexa |
|
|
|
cDNA - Plant Normal Tissue: cDNA - Plant: Corn |
C1634330 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Rice |
C1634360 |
Biochain |
40 reactions |
EUR 674 |
NPTN Recombinant Protein (Rat) |
RP214388 |
ABM |
100 ug |
Ask for price |
cDNA - Plant Normal Tissue: cDNA - Plant: Wheat |
C1634390 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Orange |
C1634340 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Potato |
C1634350 |
Biochain |
40 reactions |
EUR 674 |
NPTN Recombinant Protein (Mouse) |
RP154784 |
ABM |
100 ug |
Ask for price |
NPTN Recombinant Protein (Human) |
RP041734 |
ABM |
100 ug |
Ask for price |
Human Neuroplastin (NPTN) Protein |
20-abx654543 |
Abbexa |
-
EUR 693.60
-
EUR 309.60
-
EUR 2064.00
-
EUR 828.00
-
EUR 510.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis |
C1634310 |
Biochain |
40 reactions |
EUR 674 |
Nptn ORF Vector (Rat) (pORF) |
ORF071464 |
ABM |
1.0 ug DNA |
EUR 607.2 |
pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector |
CD111B-1 |
SBI |
10 ug |
EUR 572 |
NPTN Polyclonal Conjugated Antibody |
C31395 |
SAB |
100ul |
EUR 476.4 |
NPTN ORF Vector (Human) (pORF) |
ORF013912 |
ABM |
1.0 ug DNA |
EUR 424.8 |
Nptn ORF Vector (Mouse) (pORF) |
ORF051596 |
ABM |
1.0 ug DNA |
EUR 607.2 |
PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector |
PB511B-1 |
SBI |
10 ug |
EUR 638 |
PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector |
PB512B-1 |
SBI |
10 ug |
EUR 638 |
cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean |
C1634370 |
Biochain |
40 reactions |
EUR 674 |
pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector |
CD110B-1 |
SBI |
10 ug |
EUR 572 |
PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector |
PB510B-1 |
SBI |
10 ug |
EUR 638 |
PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector |
PB530A-2 |
SBI |
10 ug |
EUR 638 |
PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
PB531A-2 |
SBI |
10 ug |
EUR 638 |
PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector |
PB533A-2 |
SBI |
10 ug |
EUR 638 |
pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector |
CD512B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector |
CD514B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
CD510B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector |
CD530A-2 |
SBI |
10 ug |
EUR 590 |
pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
CD531A-2 |
SBI |
10 ug |
EUR 590 |
pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector |
CD533A-2 |
SBI |
10 ug |
EUR 590 |
pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector |
CD630A-1 |
SBI |
10 ug |
EUR 590 |
pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector |
CD711B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
CD515B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector |
CD522A-1 |
SBI |
10 ug |
EUR 590 |
pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
CD615B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector |
CD532A-2 |
SBI |
10 ug |
EUR 590 |
pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
CD710B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector |
CD731B-1 |
SBI |
10 ug |
EUR 590 |
PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector |
PB514B-2 |
SBI |
10 ug |
EUR 638 |
NPTN ELISA Kit (Human) (OKCD08220) |
OKCD08220 |
Aviva Systems Biology |
96 Wells |
EUR 1170 |
Description: Description of target: Neuroplastin is a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. The alpha and beta transcripts show differential localization within the brain.Neuroplastin is a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. The alpha and beta transcripts show differential localization within the brain.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.058ng/mL |
NPTN ELISA Kit (Human) (OKDD00432) |
OKDD00432 |
Aviva Systems Biology |
96 Wells |
EUR 1170 |
Description: Description of target: This gene encodes a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. Alternative splicing results in multiple transcript variants.;Species reactivity: Human;Application: ;Assay info: Quantitative Sandwich ELISA;Sensitivity: < 0.052 ng/mL |
Human NPTN Protein Lysate 20ug |
IHUNPTNPLLY20UG |
Innovative research |
each |
EUR 213 |
|
Description: Human NPTN Protein Lysate 20ug |
pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector |
CD511B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector |
CD524A-1 |
SBI |
10 ug |
EUR 590 |
Rat Neuroplastin (NPTN) ELISA Kit |
abx391703-96tests |
Abbexa |
96 tests |
EUR 1093.2 |
|
PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector |
PB513B-1 |
SBI |
10 ug |
EUR 638 |
pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged] |
CD512VA-1 |
SBI |
>1 x 10^6 IFUs |
EUR 720 |
Human Neuroplastin (NPTN) CLIA Kit |
20-abx493824 |
Abbexa |
-
EUR 9567.60
-
EUR 5095.20
-
EUR 1177.20
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
PB713B-1 |
SBI |
10 ug |
EUR 638 |
Human Neuroplastin (NPTN) ELISA Kit |
20-abx152508 |
Abbexa |
-
EUR 8853.60
-
EUR 4719.60
-
EUR 1093.20
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Human Neuroplastin (NPTN) ELISA Kit |
abx257574-96tests |
Abbexa |
96 tests |
EUR 764.4 |
|
Mouse Neuroplastin (NPTN) ELISA Kit |
abx390037-96tests |
Abbexa |
96 tests |
EUR 1093.2 |
|
Human Neuroplastin (NPTN) ELISA Kit |
DLR-NPTN-Hu-48T |
DL Develop |
48T |
EUR 620.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Neuroplastin (NPTN) in samples from tissue homogenates or other biological fluids. |
Human Neuroplastin (NPTN) ELISA Kit |
DLR-NPTN-Hu-96T |
DL Develop |
96T |
EUR 807.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Neuroplastin (NPTN) in samples from tissue homogenates or other biological fluids. |
Human NPTN(Neuroplastin) ELISA Kit |
EH4175 |
FN Test |
96T |
EUR 628.92 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
Human Neuroplastin (NPTN) ELISA Kit |
RD-NPTN-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 625.2 |
Human Neuroplastin (NPTN) ELISA Kit |
RD-NPTN-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 867.6 |
Human Neuroplastin (NPTN) ELISA Kit |
RDR-NPTN-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 652.8 |
Human Neuroplastin (NPTN) ELISA Kit |
RDR-NPTN-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 907.2 |
Human Neuroplastin (NPTN) ELISA Kit |
RDR-NPTN-Hu-48T |
Reddot Biotech |
48T |
EUR 475.34 |
|
Description: tissue homogenates and other biological fluids. |
Human Neuroplastin (NPTN) ELISA Kit |
RDR-NPTN-Hu-96T |
Reddot Biotech |
96T |
EUR 679.04 |
|
Description: tissue homogenates and other biological fluids. |
Human Neuroplastin (NPTN) ELISA Kit |
RD-NPTN-Hu-48T |
Reddot Biotech |
48T |
EUR 452.7 |
|
Description: tissue homogenates and other biological fluids. |
Human Neuroplastin (NPTN) ELISA Kit |
RD-NPTN-Hu-96T |
Reddot Biotech |
96T |
EUR 646.7 |
|
Description: tissue homogenates and other biological fluids. |
Human Neuroplastin (NPTN) ELISA Kit |
DL-NPTN-Hu |
DL Develop |
96T |
EUR 441 |
|
Description: tissue homogenates or other biological fluids. |
Human Neuroplastin (NPTN) ELISA Kit |
DLR-NPTN-Hu |
DL Develop |
96T |
EUR 463 |
|
Description: tissue homogenates or other biological fluids. |
Human NPTN(Neuroplastin) ELISA Kit |
ELK4704-48T |
ELK Biotech |
48T |
Ask for price |
|
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NPTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NPTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPTN in the samples is then determined by comparing the OD of the samples to the standard curve. |
Human NPTN(Neuroplastin) ELISA Kit |
ELK4704-96T |
ELK Biotech |
96T |
Ask for price |
|
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NPTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NPTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPTN in the samples is then determined by comparing the OD of the samples to the standard curve. |
pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
CD513B-1 |
SBI |
10 ug |
EUR 572 |
Novo? cDNA Kit |
M1165-100 |
Biovision |
each |
EUR 424.8 |
Novo? cDNA Kit |
M1165-25 |
Biovision |
each |
EUR 320.4 |
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector |
CD516B-2 |
SBI |
10 ug |
EUR 572 |
pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector |
CD713B-1 |
SBI |
10 ug |
EUR 572 |
cDNA - Lupus: Lung |
C1236152Lup |
Biochain |
40 reactions |
EUR 737 |
cDNA - Lupus: Colon |
C1236090Lup |
Biochain |
40 reactions |
EUR 737 |
cDNA - Lupus: Heart |
C1236122Lup |
Biochain |
40 reactions |
EUR 737 |
cDNA - Lupus: Liver |
C1236149Lup |
Biochain |
40 reactions |
EUR 737 |
cDNA - Asthma: Lung |
C1236152Ld-1 |
Biochain |
40 reactions |
EUR 899 |
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged] |
CD516VB-2 |
SBI |
>1 x 10^6 IFUs |
EUR 706 |
h NPTN inducible lentiviral particles |
LVP406 |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 541.2 |
Description: Pre-made inducible lentiviral particles for expressing human target: NPTN (alternative name: GP55; GP65; SDR1; np55; np65; SDFR1), with ORF sequence 100% matching to CDS region in NCBI ID: NM_017455. |
cDNA - Lupus: Kidney |
C1236142Lup |
Biochain |
40 reactions |
EUR 737 |
cDNA - Lupus: Spleen |
C1236246Lup |
Biochain |
40 reactions |
EUR 737 |
Evo? cDNA Supermix |
M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
each |
EUR 320.4 |
pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg |
CD527A-1 |
SBI |
10 ug |
EUR 590 |
cDNA - Lupus: stomach |
C1236248Lup |
Biochain |
40 reactions |
EUR 737 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
each |
EUR 346.8 |
cDNA - Emphysema: Lung |
C1236152Ld-3 |
Biochain |
40 reactions |
EUR 899 |
cDNA - Pneumonia: Lung |
C1236152Ld-4 |
Biochain |
40 reactions |
EUR 899 |
cDNA - Lupus: Pancreas |
C1236188Lup |
Biochain |
40 reactions |
EUR 737 |
pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged] |
CD513VB-1 |
SBI |
>1 x 10^6 IFUs |
EUR 720 |
cDNA - Bronchitis: Lung |
C1236152Ld-2 |
Biochain |
40 reactions |
EUR 899 |
pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus] |
CD511VB-1 |
SBI |
>1 x 10^6 IFUs |
EUR 720 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
20-abx09801620ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
20-abx09802120ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
cDNA - hypertension: Vein |
C1236020Hd-2 |
Biochain |
40 reactions |
EUR 729 |
cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
cDNA - hypertension: Heart |
C1236122Hd-2 |
Biochain |
40 reactions |
EUR 729 |
Evo™ cDNA Kit |
M1164-100 |
Biovision |
each |
Ask for price |
Evo™ cDNA Kit |
M1164-25 |
Biovision |
each |
Ask for price |
ELISA kit for Human NPTN (Neuroplastin) |
ELK4704 |
ELK Biotech |
1 plate of 96 wells |
EUR 518.4 |
|
Description: A sandwich ELISA kit for detection of Neuroplastin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Nptn sgRNA CRISPR Lentivector set (Rat) |
K6791401 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
cDNA - hypertension: Artery |
C1236013Hd-2 |
Biochain |
40 reactions |
EUR 729 |
cDNA - hypertension: Kidney |
C1236142Hd-2 |
Biochain |
40 reactions |
EUR 729 |
Nptn 3'UTR GFP Stable Cell Line |
TU264142 |
ABM |
1.0 ml |
Ask for price |
Nptn 3'UTR GFP Stable Cell Line |
TU164271 |
ABM |
1.0 ml |
Ask for price |
NPTN 3'UTR GFP Stable Cell Line |
TU065917 |
ABM |
1.0 ml |
EUR 1672.8 |
Nptn sgRNA CRISPR Lentivector set (Mouse) |
K4866601 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
NPTN sgRNA CRISPR Lentivector set (Human) |
K1449901 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
cDNA - Arteriosclerosis: Aorta |
C1236012Hd-4 |
Biochain |
40 reactions |
EUR 899 |
cDNA - Arteriosclerosis: Artery |
C1236013Hd-4 |
Biochain |
40 reactions |
EUR 899 |
NPTN Protein Vector (Rat) (pPM-C-HA) |
PV285856 |
ABM |
500 ng |
EUR 723.6 |
pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector |
CD550A-1 |
SBI |
10 ug |
EUR 563 |
NPTN Protein Vector (Rat) (pPB-C-His) |
PV285854 |
ABM |
500 ng |
EUR 723.6 |
NPTN Protein Vector (Rat) (pPB-N-His) |
PV285855 |
ABM |
500 ng |
EUR 723.6 |
NPTN Protein Vector (Rat) (pPM-C-His) |
PV285857 |
ABM |
500 ng |
EUR 723.6 |
cDNA - Lupus: Spinal Cord |
C1236234Lup |
Biochain |
40 reactions |
EUR 737 |
NPTN Protein Vector (Human) (pPM-C-HA) |
PV055647 |
ABM |
500 ng |
EUR 577.2 |
NPTN Protein Vector (Mouse) (pPM-C-HA) |
PV206384 |
ABM |
500 ng |
EUR 723.6 |
Furthermore, yeast RNase H2 orthologous mutants of two widespread RNase H2 defects related to Aicardi Goutières syndrome (AGS) in people, displayed elevated cDNA-driven restore of a DSB when mixed with one another or with RNase H1 null mutation. Our findings assist the speculation that faulty RNase H2 alleles have larger stage of cDNA derived from both coding or non-coding RNA within the type of RNA-cDNA hybrids.