Tag: immunohistochemistry troubleshooting

DNA Replication-Transcription Conflicts Do Not Significantly Contribute to Spontaneous Mutations Due to Replication Errors in Escherichia coli

DNA Replication-Transcription Conflicts Do Not Significantly Contribute to Spontaneous Mutations Due to Replication Errors in Escherichia coliDNA Replication-Transcription Conflicts Do Not Significantly Contribute to Spontaneous Mutations Due to Replication Errors in Escherichia coli

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Encounters between DNA replication and transcription could cause genomic disruption, significantly when the 2 meet head-on. Whether or not these conflicts produce level mutations is debated. This paper presents detailed

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Visible Immunoprecipitation (VIP) Assay: a Simple and Versatile Method forVisual Detection of Protein-protein Interactions

Visible Immunoprecipitation (VIP) Assay: a Simple and Versatile Method forVisual Detection of Protein-protein InteractionsVisible Immunoprecipitation (VIP) Assay: a Simple and Versatile Method forVisual Detection of Protein-protein Interactions

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The seen immunoprecipitation (VIP) assay is a handy various to standard co-immunoprecipitation ( Katoh et al., 2015 ). By processing lysates from cells co-expressing GFP-fusion and RFP-fusion proteins for immunoprecipitation with GST-tagged anti-GFP

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