NAD⁺ is a redox cofactor important to the right functioning of a wide range of essential metabolic pathways, together with key steps in mitochondrial vitality metabolism. As well as, it serves as a signaling substrate for enzymes reminiscent of sirtuins and the poly-ADP ribosyl-polymerase household of enzymes.

Sirtuins, that are NAD⁺-dependent protein deacylases, harness modifications in mobile NAD⁺ concentrations to provide modifications in protein acylation standing, thereby affecting downstream features together with vitality metabolism, stress resistance, and cell survival.

Thus, the provision of NAD⁺ in cells, or in particular organelles such because the mitochondrion, regulates downstream signaling and key organic processes. This idea has pushed a necessity for researchers to simply and exactly measure NAD⁺ concentrations in organic samples.

Paraffin Wax Dispenser

HIS7000

Paraffin wax, granular (56 - 60)

GL4115-1KG

Paraffin wax, granular (56 - 60)

GL4115-5KG

8KG Histoplast PE Paraffin

HIS3324

Paraffin oil, BP, Ph. Eur. grade

GL4141-1L

We herein describe a number of protocols for the measurement of NAD⁺ and NADH concentrations in tissues, cells, or subcellular compartments reminiscent of mitochondria. These protocols embody a biking assay that may shortly measure NAD⁺ or NADH ranges utilizing a plate reader outfitted with fluorescence measurement capabilities.

This plate assay depends solely upon commercially out there supplies along with the organic samples of curiosity. As well as, we describe a protocol using steady isotope-labeled NAD⁺ as an inside customary to find out organic NAD⁺ content material by isotope-dilution strategies. This technique requires mass spectrometry to ratio endogenous NAD⁺ with exogenous isotope-labeled NAD⁺ to acquire quantification utilizing HPLC and mass spectrometry.

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D	Bioingentech
MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D	Bioingentech
MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-48D	Bioingentech
MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-96D	Bioingentech
Blood PCR Kit
20-abx09801320ulSystems	Abbexa

A novel and environment friendly light-controlled colorimetric assay for the quantification and detection of nitroreductase (NTR) was constructed primarily based on p-aminophenol (pAP)-catalyzed and nicotinamide adenine dinucleotide (NADH)-mediated technology of AgNPs.

Because of the hydrolysis of p-nitrophenol by NTR within the presence of NADH, the hydrolysis product can be utilized as a catalyst to catalyze the discount of Ag+ by NADH beneath the sunshine. Because the focus of NTR will increase, the worth of absorbance at ca. 400 nm (A400) decreases and the colour of the answer turns from brown to vivid yellow.

A linear correlation was obtained between A400 and the NTR focus within the vary from 1-50 μg mL-1 and the restrict of detection (LOD) is 0.27 μg mL-1.

Lastly, we utilized the assay to find out NTR in human serum samples by spiking totally different concentrations of NTR with a restoration of 85.2%-92.5%.

Pir ELISA Kit| Rat Pirin ELISA Kit

EF019186

REN ELISA Kit| Rat Renin ELISA Kit

EF017245

Kl ELISA Kit| Rat Klotho ELISA Kit

EF017474

Understanding of microbial metallic discount relies nearly solely on research of Gram-negative organisms. On this research, we deal with Desulfotomaculum reducens MI-1, a Gram-positive metallic reducer whose genome lacks genes with similarity to any characterised metallic reductase.

Utilizing non-denaturing separations and mass spectrometry identification, together with a colorimetric display screen for chelated Fe(III)-NTA discount with NADH as electron donor, we’ve got recognized proteins from the D. reducens proteome not beforehand characterised as iron reductases.

Their perform was confirmed by heterologous expression in Escherichia coli. Moreover, we present that these proteins have the aptitude to scale back soluble Cr(VI) and U(VI) with NADH as electron donor. The proteins recognized are NADH : flavin oxidoreductase (Dred_2421) and a protein complicated composed of oxidoreductase flavin adenine dinucleotide/NAD(P)-binding subunit (Paraffin Wax) and dihydroorotate dehydrogenase 1B (Dred_1686).

Dred_2421 was recognized within the soluble proteome and is predicted to be a cytoplasmic protein. Dred_1685 and Dred_1686 had been recognized in each the soluble in addition to the insoluble protein fraction, suggesting a sort of membrane affiliation, though PSORTb predicts each proteins are cytoplasmic. This research is the primary purposeful proteomic evaluation of D. reducens and one of many first analyses of metallic and radionuclide discount in an environmentally related Gram-positive bacterium.

Pir ELISA Kit| Rat Pirin ELISA Kit
EF019186	Lifescience Market
REN ELISA Kit| Rat Renin ELISA Kit
EF017245	Lifescience Market
Kl ELISA Kit| Rat Klotho ELISA Kit
EF017474	Lifescience Market
LN ELISA Kit| Rat Laminin ELISA Kit
EF017041	Lifescience Market
Lep ELISA Kit| Rat Leptin ELISA Kit
EF017074	Lifescience Market

Biosynthesis of poly-3-hydroxybutyrate (PHB) as a fermentation product allows the coupling of progress and product technology. Furthermore, the discount of oxygen provide ought to cut back operative value and enhance product yield.

Era of PHB as a fermentation product relies on the in vivo exercise of an NADH-preferring acetoacetyl-CoA reductase.

Proof of this idea requires

(i) quantification of the cofactor desire, in physiologically related circumstances, of a putative NADH-preferring acetoacetyl-CoA reductase and

(ii) verification of PHB accumulation utilizing an NADH-preferring acetoacetyl-CoA reductase in a species naturally incapable of doing so, for instance, Escherichia coli.

This dataset comprises kinetic knowledge obtained by spectrophotometry and knowledge from a steady tradition of an engineered E. coli pressure accumulating PHB beneath oxygen-limiting circumstances.

On this dataset it’s attainable to search out

(1) enzyme stability assays;

(4) estimations of the flux capability of the response catalyzed by an acetoacetyl-CoA reductase;

(7) metabolic fluxes distributions throughout the steady progress of this engineered pressure.

As a result of a comparatively small variety of acetoacetyl-CoA reductases have been kinetically characterised, knowledge and scripts right here offered may very well be helpful for additional kinetic characterizations.

Furthermore, the process described to estimate biomass composition may very well be fascinating to estimate plasmid and protein burden in different strains. Software of knowledge reconciliation to fermentations ought to assist to acquire particular charges in step with the precept of mass and electron conservation.

All of the required knowledge and scripts to carry out these analyses are deposited in a Mendeley Knowledge repository. This text was co-submitted with the manuscript entitled “An NADH preferring acetoacetyl-CoA reductase is engaged in poly-3-hydroxybutyrate accumulation in Escherichiasia. coli”.

Pir ELISA Kit| Rat Pirin ELISA Kit

Lifescience Market

REN ELISA Kit| Rat Renin ELISA Kit

Lifescience Market

Kl ELISA Kit| Rat Klotho ELISA Kit

Lifescience Market

A Widespread Primer Multiplex PCR (CP-M-PCR) was developed to detect meat origin of 4 teams of animal (pig, ruminant, avian and rabbit). This technique demonstrated increased sensitivity and effectivity than the traditional multiplex PCR.

On this method, a standard ahead primer was designed within the 5′ finish of a homologous area of mitochondrial NADH dehyrogenase subunit 4 (Nad 4) gene sequences of all of the animal teams. Particular adapter reverse primers had been designed by including an adapter sequence on the 5′ finish.

The identical adapter sequence was used because the widespread adapter reverse primer. The primers generated particular fragments of 267, 370, 504, and 548 bp lengths for pig, ruminant, avian and rabbit meats, respectively. The usage of adapter sequence on the 5′ finish of the widespread adapter reverse primers elevated the effectivity of the amplification and the appliance of a standard ahead primer solved the complexity in multiplex PCR system.

Bands of particular amplification will be detected within the PCR assays containing as little as 10(-6) μM of adapter reverse primer. This end result indicated that the sensitivity was tremendously elevated as in comparison with the traditional multiplex PCR (10(-3) μM).

EnzyChrom™ NAD/NADH Assay Kit
E2ND-100	BioAssay Systems	100	EUR 479
Description: For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 µM and linearity up to 10 µM NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Cell or tissue extracts. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.05 µM.

Amplite® Colorimetric NADH Assay Kit
15271	AAT Bioquest	400 Tests	EUR 278

Amplite® Colorimetric NADH Assay Kit
15271-400Tests	AAT Bioquest	400 Tests	EUR 278
Description: Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

PicoProbe? NADH Fluorometric Assay Kit
K338-100	Biovision	each	EUR 692.4

Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*
15261	AAT Bioquest	400 Tests	EUR 334

Amplite® Colorimetric Total NAD and NADH Assay Kit
15258	AAT Bioquest	400 Tests	EUR 334

Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*
15261-400Tests	AAT Bioquest	400 Tests	EUR 334
Description: Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

Amplite® Colorimetric Total NAD and NADH Assay Kit
15258-400Tests	AAT Bioquest	400 Tests	EUR 334
Description: Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

Amplite® Colorimetric NAD/NADH Ratio Assay Kit
15273	AAT Bioquest	250 Tests	EUR 390

Amplite® Colorimetric NAD/NADH Ratio Assay Kit
15273-250Tests	AAT Bioquest	250 Tests	EUR 390
Description: Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

EZScreen? NAD+/NADH Colorimetric Assay Kit (384-well)
K958-400	Biovision	each	EUR 777.6

Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*
15257	AAT Bioquest	400 Tests	EUR 334

Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*
15257-400Tests	AAT Bioquest	400 Tests	EUR 334
Description: Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

Amplite® Colorimetric Total NAD and NADH Assay Kit *Enhanced Sensitivity*
15275	AAT Bioquest	400 Tests	EUR 334

Amplite® Colorimetric Total NAD and NADH Assay Kit *Enhanced Sensitivity*
15275-400Tests	AAT Bioquest	400 Tests	EUR 334
Description: Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*
15263	AAT Bioquest	250 Tests	EUR 390

Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*
15263-250Tests	AAT Bioquest	250 Tests	EUR 390
Description: Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

NADPase Assay Kit
abx097983-100Assays	Abbexa	100 Assays	EUR 604.8

NADP / NADPH Assay Kit
abx096008-100Assays	Abbexa	100 Assays	EUR 566.4

NADP+/NADPH Assay Kit
MET-5018	Cell Biolabs	100 assays	EUR 686.4
Description: Our NADP+/NADPH Assay Kit detects NADP+ and NADPH in cell and tissue lysates. Total NADP+/NADPH can be detected or samples can be treated with an acid or base treatment to specifically detect NADP+ or NADPH. This assay uses an enzymatic cycling reaction that reduces NADP+ to NADPH, which then reacts with a colorimetric probe and is detected with a spectrophotometric plate reader at 450nm. NADP+/ NADPH levels in unknown samples are calculated based on the provided NADP+ standard curve.

NADP/NADPH Assay Kit
Z5030038	Biochain	100 assays	EUR 805

NAD Kinase Assay Kit
abx298807-100Assays	Abbexa	100 Assays	EUR 661.2

EnzyFluo NADP/NADPH Assay Kit
EFNP-100	BioAssay Systems	100	EUR 537.6
Description: Sensitive determination of NADP and NADPH by fluorometric (530/585nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 0.01 µM. Shelf life: 6 months. Shipping: on ice; storage: -20°C.

EnzyChrom NADP/NADPH Assay Kit
ECNP-100	BioAssay Systems	100	EUR 537.6
Description: Quantitative determination of NADP and NADPH by colorimetric (565nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 0.1 µM. Shelf life: 6 months. Shipping: on ice; storage: -20°C.

MicroMolar NADPH Assay Kit
NADPH100	ProFoldin	100 assays	EUR 221.78
Description: This product includes 1 ml of 10x Assay buffer, 0.1 ml of 20 x Reagent A, 0.1 ml of 20 x Reagent B, 0.5 ml of 10x Reagent C and 0.020 ml of 10 mM NADPH.

NADP+/NADPH Assay Kit (Fluorometric)
MET-5031	Cell Biolabs	100 assays	EUR 686.4
Description: Our NADP+/NADPH Assay Kit detects NADP+ and NADPH in cell and tissue lysates. Total NADP+/NADPH can be detected or samples can be treated with an acid or base treatment to specifically detect NADP+ or NADPH. This assay uses an enzymatic cycling reaction that reduces NADP+ to NADPH, which then reacts with a fluorometric probe and is detected with a fluorescence plate reader at 530-570nm excitation and 590-600nm emission. NADP+/ NADPH levels in unknown samples are calculated based on the provided NADP+ standard curve.

EnzyFluo™ NADP/NADPH Assay Kit
EF2NP-100	BioAssay Systems	100	EUR 479
Description: For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism (This product is a replacement for the discontinued product EFNP-100). Key Features: Sensitive and accurate. Detection limit of 0.01 µM and linearity up to 1 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm. Samples: Cell, tissue extracts etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.01 µM.

EnzyChrom™ NADP/NADPH Assay Kit
E2NP-100	BioAssay Systems	100	EUR 479
Description: For quantitative determination of NADP+/NADPH concentrations and ratios in cell or tissue extracts. Key Features: Sensitive and accurate. Detection limit 0.1 µM, linearity up to 10 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Colorimetric Determination at 565 nm. Samples: Cells, Tissue. Species: human, mouse, rat and more. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 µM.

NADH Na2
20-abx082105	Abbexa	EUR 343.20 EUR 260.40	1 g 250 mg

XTT CELL VIABILITY ASSAY KIT (1000 ASSAY)
30007	Biotium	1KIT	EUR 283.2
Description: Minimum order quantity: 1 unit of 1KIT

NADH-Inhibitor
Y4681000	Bio Basic	10mg	EUR 240.96

E. coli NAD+ Kinase Assay Kit Plus
NADK100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes 600 ul of 10 x assay buffer, 35 ul of 100 x NAD+, 35 ul of 100 x E. coli NAD kinase, 35 ul of 100 x ATP, 35 ul of 100 x MUK Reagent A, 35 ul of 100 x MUK Reagent B and 300 ul of 10 x fluorescence dye for 100 assays in a 384-well assay format.

Amplite® Colorimetric NADPH Assay Kit
15272	AAT Bioquest	400 Tests	EUR 278

Amplite® Colorimetric NADPH Assay Kit
15272-400Tests	AAT Bioquest	400 Tests	EUR 278
Description: Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

NADPH-Cytochrome c Reductase Assay Kit
abx097996-100Assays	Abbexa	100 Assays	EUR 698.4

E. coli NAD+ Kinase Assay Kit Plus-500
NADK500KE	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 170 ul of 100 x NAD+, 170 ul of 100 x E. coli NAD kinase, 170 ul of 100 x ATP, 170 ul of 100 x MUK Reagent A, 170 ul of 100 x MUK Reagent B and 1.7 ml of 10 x fluorescence dye for 500 assays in a 384-well assay format.

Amplite® Colorimetric NADP/NADPH Ratio Assay Kit
15274	AAT Bioquest	250 Tests	EUR 390

Amplite® Colorimetric NADP/NADPH Ratio Assay Kit
15274-250Tests	AAT Bioquest	250 Tests	EUR 390
Description: Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

NADH, disodium salt
2735-1000	Biovision	each	EUR 202.8

CP-M-PCR detection restrict of the DNA samples was 0.1 ng for the 4 teams of meats. CP-M-PCR has enormously improved the sensitivity and effectivity of the PCR system for a extra dependable and correct final result than typical multiplex PCR system.

Pir ELISA Kit| Rat Pirin ELISA Kit

EF019186

REN ELISA Kit| Rat Renin ELISA Kit

EF017245

Kl ELISA Kit| Rat Klotho ELISA Kit

EF017474