A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol

A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol

Crosslinking and immunoprecipitation (CLIP) strategies are highly effective strategies to interrogate direct protein-RNA interactions and dissect posttranscriptional gene regulatory networks. One broadly used CLIP variant is photoactivatable ribonucleoside enhanced CLIP (PAR-CLIP) that entails in vivo labeling of nascent RNAs with the photoreactive nucleosides 4-thiouridine (4SU) or 6-thioguanosine (6SG), which might effectively crosslink to interacting proteins utilizing UVA and UVB mild.

Crosslinking of 4SU or 6SG to interacting amino acids adjustments their base-pairing properties and leads to attribute mutations in cDNA libraries ready for high-throughput sequencing, which could be computationally exploited to take away ample background from non-crosslinked sequences and assist pinpoint RNA binding protein binding websites at nucleotide decision on a transcriptome-wide scale.

Right here we current a streamlined protocol for fluorescence-based PAR-CLIP (fPAR-CLIP) that eliminates the necessity to use radioactivity. It’s primarily based on direct ligation of a fluorescently labeled adapter to the three’finish of crosslinked RNA on immobilized ribonucleoproteins, adopted by isolation of the adapter-ligated RNA and environment friendly conversion into cDNA with out the beforehand wanted measurement fractionation on denaturing polyacrylamide gels. These enhancements reduce the experimentation by half to 2 days and will increase sensitivity by 10-100-fold.

Characterization and evaluation of the transcriptome response to drought in Larix kaempferi utilizing PacBio full-length cDNA sequencing built-in with de novo RNA-seq reads

A hypothetical mannequin of drought tolerance mechanism of Larix kaempferi was established via SMRT-seq and Illumina HiSeq. Larix kaempferi is a vital financial and ecological species and a significant afforestation species in north-eastern China. Up to now, no info has been reliably derived concerning full-length cDNA sequencing info on L. kaempferi. By single-molecule long-read isoform sequencing (SMRT-seq), right here we report a complete of 26,153,342 subreads (21.24 Gb) and 330,371 round consensus sequence (CCS) reads after the modification of web site mismatch, and 35,414 unigenes have been efficiently collected.

To achieve deeper insights into the molecular mechanisms of L. kaempferi response to drought stress, we mixed Illumina HiSeq with SMRT-seq to decode full-length transcripts. On this examine, we report 27 differentially expressed genes (DEGs) concerned within the notion and transmission of drought stress indicators in L. kaempferi.

Numerous DEGs responding to drought stress have been detected in L. kaempferi, particularly DEGs concerned within the reactive oxygen species (ROS) scavenging, lignin biosynthesis, and sugar metabolism, and DEGs encoding drought stress proteins. We detected 73 transcription components (TFs) below drought stress, together with AP2/ERF, bZIP, TCP, and MYB. This examine offers primary full sequence sources for L. kaempferi analysis and can assist us to raised perceive the capabilities of drought-resistance genes in L. kaempferi.

A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol

cDNA-derived RNA phage meeting reveals important residues within the maturation protein of the Pseudomonas aeruginosa leviphage, PP7

PP7 is a leviphage with single-stranded RNA genome, which infects Pseudomonas aeruginosa PAO1. A reverse genetic system for PP7 was beforehand created through the use of reverse-transcribed cDNA (PP7O) from virion-derived RNA genome. Right here, we’ve discovered that the PP7O cDNA contained 20 nucleotide variations from the PP7 genome sequence deposited within the database.

We created one other reverse genetic system exploiting chemically synthesized cDNA (PP7S) primarily based on the database sequence. In contrast to PP7O that rendered infectious PP7 virions, PP7S-derived particles have been incapable of plaque formation on PAO1 cells, which was restored on the PAO1 cells expressing the maturation protein (MP) from PP7O Utilizing this reverse genetic system, we revealed two amino acid residues concerned within the identified roles of MP (i.e. adsorption and genome replication), fortuitously offering a lesson that the viral RNA genome sequencing wants purposeful verification presumably by a reverse genetic system.

IMPORTANCE Organic significance of RNA phages has been largely ignored, mockingly as a result of few research have been specializing in RNA phages. As an preliminary try to correctly signify RNA phages within the phageome, we beforehand created, through the use of reverse-transcribed cDNA, a reverse genetic system for the small RNA phage, PP7 that infects the opportunistic human pathogen, Pseudomonas aeruginosa We right here report one other system through the use of chemically synthesized cDNA primarily based on the database genome that has 20 nucleotide variations from the earlier cDNA.

Investigation of these cDNA-derived phage virions unveiled that two amino acids of the maturation protein are essential for the traditional phage lifecycle at totally different steps. Our examine offers an perception into the molecular foundation for the RNA phage lifecycle and a lesson that the RNA genome sequencing must be rigorously validated by cDNA-based phage meeting methods.

RNAcDNA hybrids mediate transposition by way of totally different mechanisms

Retrotransposons can signify half of eukaryotic genomes. Retrotransposon dysregulation destabilizes genomes and has been linked to varied human ailments. Rising regulators of retromobility embrace RNA-DNA hybrid-containing constructions generally known as R-loops. Accumulation of those constructions on the transposons of yeast 1 (Ty1) parts has been proven to extend Ty1 retromobility via an unknown mechanism. Right here, by way of a focused genetic display screen, we recognized the rnh1Δ rad27Δ yeast mutant, which lacked each the Ty1 inhibitor Rad27 and the RNA-DNA hybrid suppressor Rnh1.

The mutant exhibited elevated ranges of Ty1 cDNA-associated RNA-DNA hybrids that promoted Ty1 mobility. Furthermore, on this rnh1Δ rad27Δ mutant, however not within the double RNase H mutant rnh1Δ rnh201Δ, RNA-DNA hybrids preferentially existed as duplex nucleic acid constructions and elevated Ty1 mobility in a Rad52-dependent method.

POLR3F siRNA

20-abx929232
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  • 15 nmol
  • 30 nmol

POLR3F Peptide

4727P 0.05 mg
EUR 197.7
Description: (NT) POLR3F peptide

POLR3F Antibody

24726 100ul
EUR 479

POLR3F Antibody

24726-100ul 100ul
EUR 468

POLR3F Antibody

4727-002mg 0.02 mg
EUR 206.18
Description: POLR3F Antibody: The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.

POLR3F Antibody

4727-01mg 0.1 mg
EUR 523.7
Description: POLR3F Antibody: The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.

POLR3F Antibody

E38PA2497 100ul
EUR 225
Description: Available in various conjugation types.

POLR3F Antibody

E912224 100ul
EUR 255
Description: Available in various conjugation types.

POLR3F Antibody

E94441 100μg
EUR 255
Description: Available in various conjugation types.

POLR3F antibody

70R-2962 50 ug
EUR 467
Description: Rabbit polyclonal POLR3F antibody raised against the N terminal of POLR3F

POLR3F antibody

70R-50816 100 ul
EUR 242
Description: Purified Polyclonal POLR3F antibody

POLR3F antibody

70R-19415 50 ul
EUR 289
Description: Rabbit polyclonal POLR3F antibody

POLR3F antibody

70R-2038 50 ug
EUR 467
Description: Rabbit polyclonal POLR3F antibody raised against the middle region of POLR3F

POLR3F Antibody

1-CSB-PA018348GA01HU
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  • 150ul
  • 50ul
Description: A polyclonal antibody against POLR3F. Recognizes POLR3F from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

POLR3F Antibody

1-CSB-PA884440LA01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against POLR3F. Recognizes POLR3F from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000

POLR3F Antibody

GWB-MR240F 50ug Ask for price

POLR3F Antibody

GWB-MR241G 50ug Ask for price

POLR3F Rabbit pAb

E2510867 100ul
EUR 225
Description: Available in various conjugation types.

POLR3F Rabbit pAb

A12224-100ul 100 ul
EUR 369.6

POLR3F Rabbit pAb

A12224-200ul 200 ul
EUR 550.8

POLR3F Rabbit pAb

A12224-20ul 20 ul
EUR 219.6

POLR3F Rabbit pAb

A12224-50ul 50 ul
EUR 267.6

anti- POLR3F antibody

FNab06632 100µg
EUR 658.5
Description: Antibody raised against POLR3F

POLR3F Blocking Peptide

33R-5238 100 ug
EUR 119
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of POLR3F antibody, catalog no. 70R-2038

POLR3F Blocking Peptide

33R-5647 100 ug
EUR 119
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of POLR3F antibody, catalog no. 70R-2962

POLR3F Blocking Peptide

20-abx063691
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  • 1 mg
  • 5 mg

POLR3F Polyclonal Antibody

27655 100ul
EUR 439

POLR3F Polyclonal Antibody

27655-100ul 100ul
EUR 302.4

POLR3F Polyclonal Antibody

27655-50ul 50ul
EUR 224.4

POLR3F Polyclonal Antibody

A60346
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  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

POLR3F Polyclonal Antibody

A72143 50 μl
EUR 408

POLR3F Polyclonal Antibody

E-AB-18972-120uL 120uL
EUR 240
Description: Unconjugated

POLR3F Polyclonal Antibody

E-AB-18972-200uL 200uL
EUR 399
Description: Unconjugated

POLR3F Polyclonal Antibody

E-AB-18972-20uL 20uL
EUR 73
Description: Unconjugated

POLR3F Polyclonal Antibody

E-AB-18972-60uL 60uL
EUR 143
Description: Unconjugated

POLR3F Polyclonal Antibody

E-AB-52882-120uL 120uL
EUR 240
Description: Unconjugated

POLR3F Polyclonal Antibody

E-AB-52882-200uL 200uL
EUR 399
Description: Unconjugated

POLR3F Polyclonal Antibody

E-AB-52882-20uL 20uL
EUR 73
Description: Unconjugated

POLR3F Polyclonal Antibody

E-AB-52882-60uL 60uL
EUR 143
Description: Unconjugated

POLR3F protein (His tag)

30R-2961 100 ug
EUR 329
Description: Purified recombinant Human POLR3F protein (His tag)

Human POLR3F ELISA KIT

ELI-53168h 96 Tests
EUR 988.8

POLR3F ELISA KIT|Human

EF001942 96 Tests
EUR 826.8

Mouse Polr3f ELISA KIT

ELI-14354m 96 Tests
EUR 1038

Bovine POLR3F ELISA KIT

ELI-14353b 96 Tests
EUR 1113.6

Human POLR3F shRNA Plasmid

20-abx957233
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  • 150 µg
  • 300 µg

Mouse POLR3F shRNA Plasmid

20-abx977203
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  • 150 µg
  • 300 µg

Polr3f (untagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide F (Polr3f), (10ug)

MC200703 10 µg Ask for price

Polr3f (GFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide F (Polr3f)

MG204539 10 µg Ask for price

POLR3F Antibody, HRP conjugated

1-CSB-PA884440LB01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against POLR3F. Recognizes POLR3F from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

POLR3F Recombinant Protein (Rat)

RP221387 100 ug Ask for price

POLR3F Antibody, FITC conjugated

1-CSB-PA884440LC01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against POLR3F. Recognizes POLR3F from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

POLR3F Recombinant Protein (Mouse)

RP163421 100 ug Ask for price

POLR3F Recombinant Protein (Human)

RP024124 100 ug Ask for price

Rabbit Polyclonal POLR3F Antibody

TA306617 100 µg Ask for price

POLR3F Rabbit Polyclonal Antibody

E10G10090 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

POLR3F Rabbit Polyclonal Antibody

E10G03084 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

POLR3F Antibody, Biotin conjugated

1-CSB-PA884440LD01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against POLR3F. Recognizes POLR3F from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

OAPB00722-100UG - POLR3F Antibody

OAPB00722-100UG 0.1mg
EUR 389

POLR3F (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F)

SC116048 10 µg Ask for price

Polr3f ORF Vector (Rat) (pORF)

ORF073797 1.0 ug DNA
EUR 607.2

Polr3f (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide F (Polr3f)

MR204539 10 µg Ask for price

POLR3F Polyclonal Conjugated Antibody

C27655 100ul
EUR 476.4

POLR3F ORF Vector (Human) (pORF)

ORF008042 1.0 ug DNA
EUR 114

Polr3f ORF Vector (Mouse) (pORF)

ORF054475 1.0 ug DNA
EUR 607.2

Lenti ORF clone of Polr1e (mGFP-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372)

MR207725L4 10 µg Ask for price

POLR3F (GFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F)

RG203100 10 µg Ask for price

Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F), mGFP tagged

RC203100L4 10 µg Ask for price

Human POLR3F Protein Lysate 20ug

IHUPOLR3FPLLY20UG each
EUR 213
Description: Human POLR3F Protein Lysate 20ug

Lenti ORF clone of Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372)

MR207725L3 10 µg Ask for price

POLR3F (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F)

RC203100 10 µg Ask for price

OCOA12096-20UG - POLR3F Protein Lysate

OCOA12096-20UG 20ug
EUR 169

Polr3f (untagged ORF) - Rat polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (Polr3f), (10 ug)

RN202392 10 µg Ask for price

POLR3F Over-expression Lysate Product

GWB-C5647C 0.1 mg Ask for price

Polr1e (GFP-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372)

MG207725 10 µg Ask for price

POLR3F Polyclonal Antibody, HRP Conjugated

A60349
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  • 100 µg
  • 50 ul
  • 100 ul

3`UTR clone of polymerase (RNA) III (DNA directed) polypeptide F 39 kDa (POLR3F) for miRNA target validation

SC212480 10 µg Ask for price

Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F), Myc-DDK-tagged

RC203100L3 10 µg Ask for price

POLR3F Polyclonal Antibody, FITC Conjugated

A60348
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  • 100 µg
  • 50 ul
  • 100 ul

Polr1e (untagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372), (10ug)

MC217814 10 µg Ask for price

POLR3F Polyclonal Antibody, Biotin Conjugated

A60347
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  • 100 µg
  • 50 ul
  • 100 ul

Lenti ORF clone of Polr3g (mGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833)

MR201232L4 10 µg Ask for price

Lenti ORF clone of Polr2e (mGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide E (cDNA clone MGC:28579 IMAGE:4210827)

MR201273L4 10 µg Ask for price

Lenti ORF clone of Polr2h (mGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide H (cDNA clone MGC:103049 IMAGE:5042770)

MR201055L4 10 µg Ask for price

Lenti ORF clone of Polr2c (mGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide C (cDNA clone MGC:5895 IMAGE:3492868)

MR203684L4 10 µg Ask for price

Lenti ORF clone of Polr3g (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833)

MR201232L3 10 µg Ask for price

Lenti ORF clone of Polr2e (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide E (cDNA clone MGC:28579 IMAGE:4210827)

MR201273L3 10 µg Ask for price

Lenti ORF clone of Polr2h (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide H (cDNA clone MGC:103049 IMAGE:5042770)

MR201055L3 10 µg Ask for price

Lenti ORF clone of Polr2c (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide C (cDNA clone MGC:5895 IMAGE:3492868)

MR203684L3 10 µg Ask for price

POLR3F (untagged) - Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F), transcript variant 2

SC334983 10 µg Ask for price

Polr3f sgRNA CRISPR Lentivector set (Rat)

K6496101 3 x 1.0 ug
EUR 406.8

Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372)

MR207725 10 µg Ask for price

POLR3F 3'UTR GFP Stable Cell Line

TU068441 1.0 ml
EUR 1672.8

Polr3f 3'UTR GFP Stable Cell Line

TU266543 1.0 ml Ask for price

Polr3f 3'UTR GFP Stable Cell Line

TU166697 1.0 ml Ask for price

Polr3f sgRNA CRISPR Lentivector set (Mouse)

K4059301 3 x 1.0 ug
EUR 406.8

POLR3F sgRNA CRISPR Lentivector set (Human)

K1685001 3 x 1.0 ug
EUR 406.8

Polr3f (Myc-DDK-tagged ORF) - Rat polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (Polr3f), (10 ug)

RR202392 10 µg Ask for price

POLR3F Protein Vector (Rat) (pPM-C-HA)

PV295188 500 ng
EUR 723.6

AAP48705-100UG - POLR3F Peptide - middle region

AAP48705-100UG 100ug
EUR 99

Lenti ORF particles, Polr3f (GFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide F (Polr3f), 200ul, >10^7 TU/mL

MR204539L4V 200 µl Ask for price

POLR3F Protein Vector (Rat) (pPB-C-His)

PV295186 500 ng
EUR 723.6

POLR3F Protein Vector (Rat) (pPB-N-His)

PV295187 500 ng
EUR 723.6

POLR3F Protein Vector (Rat) (pPM-C-His)

PV295189 500 ng
EUR 723.6

POLR3F Protein Vector (Human) (pPM-C-HA)

PV032167 500 ng
EUR 394.8

POLR3F Protein Vector (Mouse) (pPM-C-HA)

PV217900 500 ng
EUR 723.6

Polr2h (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide H (cDNA clone MGC:8248 IMAGE:3591968), (10ug)

MC200357 10 µg Ask for price

Polr2k (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide K (cDNA clone MGC:41431 IMAGE:3468916), (10ug)

MC206567 10 µg Ask for price

Polr2b (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide B (cDNA clone MGC:47046 IMAGE:5026936), (10ug)

MC206769 10 µg Ask for price

Polr2e (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide E (cDNA clone MGC:28579 IMAGE:4210827), (10ug)

MC207091 10 µg Ask for price

Polr3g (GFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:76564 IMAGE:30464021)

MG202593 10 µg Ask for price

Polr2c (GFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide C (cDNA clone MGC:118242 IMAGE:30312083)

MG203683 10 µg Ask for price

Polr2c (GFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide C (cDNA clone MGC:5895 IMAGE:3492868)

MG203684 10 µg Ask for price

Polr2c (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide C (cDNA clone MGC:5895 IMAGE:3492868), (10ug)

MC218102 10 µg Ask for price

Polr2k (GFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide K (cDNA clone MGC:41431 IMAGE:3468916)

MG200028 10 µg Ask for price

Polr2d (GFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide D (cDNA clone MGC:8083 IMAGE:3588284)

MG200351 10 µg Ask for price

Polr2h (GFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide H (cDNA clone MGC:103049 IMAGE:5042770)

MG201055 10 µg Ask for price

The info point out that in cells missing RNA-DNA hybrid and Ty1 repressors, elevated ranges of RNA-cDNA hybrids, that are related to duplex nucleic acid constructions, increase Ty1 mobility by way of a Rad52-dependent mechanism. In distinction, in cells missing RNA-DNA hybrid repressors alone, elevated ranges of RNA-cDNA hybrids, that are related to triplex nucleic acid constructions, increase Ty1 mobility by way of a Rad52-independent course of. We suggest that duplex and triplex RNA-DNA hybrids promote transposon mobility by way of Rad52-dependent or -independent mechanisms.

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Activated CD4 + T cells and CD14 hi CD16 + monocytes correlate with antibody response following influenza virus infection in humans

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