A number of biosafety stage Three and/or 4 (BSL-3/4) pathogens are high-consequence, single-stranded RNA viruses, and their genomes, when launched into permissive cells, are infectious. Furthermore, many of those viruses are choose brokers (SAs), and their genomes are additionally thought-about SAs. Because of this, cDNAs and/or their derivatives have to be examined to make sure the absence of infectious virus and/or viral RNA earlier than switch out of the BSL-3/Four and/or SA laboratory.

This tremendously limits the capability to conduct viral genomic analysis, significantly the appliance of next-generation sequencing (NGS). Right here, we current a sequence-independent methodology to quickly amplify viral genomic RNA whereas concurrently abolishing each viral and genomic RNA infectivity throughout a number of single-stranded positive-sense RNA (ssRNA+) virus households.

The method generates barcoded DNA amplicons that vary in size from 300 to 1,000 bp, which can’t be used to rescue a virus and are secure to move at room temperature. Our barcoding method permits for as much as 288 barcoded samples to be pooled right into a single library and run throughout varied NGS platforms with out potential reconstitution of the viral genome.

Our information display that this method gives full-length genomic sequence data not solely from high-titer virion preparations however it will possibly additionally get better particular viral sequence from samples with restricted beginning materials within the background of mobile RNA, and it may be used to determine pathogens from unknown samples.

In abstract, we describe a fast, common customary working process that generates high-quality NGS libraries freed from infectious virus and infectious viral RNA. IMPORTANCE This report establishes and validates a typical working process (SOP) for choose brokers (SAs) and different biosafety stage Three and/or 4 (BSL-3/4) RNA viruses to quickly generate noninfectious, barcoded cDNA amenable for next-generation sequencing (NGS).

This eliminates the burden of testing all processed samples derived from high-consequence pathogens previous to switch from high-containment laboratories to lower-containment services for sequencing. Our established protocol might be scaled up for high-throughput sequencing of tons of of samples concurrently, which might dramatically scale back the associated fee and energy required for NGS library building. NGS information from this SOP can present full genome protection from viral shares and also can detect virus-specific reads from restricted beginning materials. Our information counsel that the process might be carried out and simply validated by institutional biosafety committees throughout analysis laboratories.

PAR-CLIP and streamlined small RNA cDNA library preparation protocol for the identification of RNA binding protein goal websites.

The research of protein-RNA interactions is essential for our understanding of mobile processes and regulatory circuits managed by RNA binding proteins (RBPs). Latest subsequent era sequencing-based approaches considerably promoted our understanding of RNA biology and its significance for cell operate. We current a streamlined protocol for Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP), a method that permits for the characterization of RBP binding websites on the right track RNAs at nucleotide decision and transcriptome-wide scale.

PAR-CLIP entails irreversible UV-mediated crosslinking of RNAs labeled with photoreactive nucleosides to interacting proteins, adopted by stringent purification steps and the conversion of crosslinked RNA into small RNA cDNA libraries suitable with next-generation sequencing. The defining hallmark of PAR-CLIP is a diagnostic mutation on the crosslinking website that’s launched into cDNA in the course of the library preparation course of. This characteristic permits for environment friendly computational removing of contaminating sequences derived from non-crosslinked fragments of ample mobile RNAs.

Within the following, we current two completely different step-by-step procedures for PAR-CLIP, which differ within the small RNA cDNA library preparation process: (1) Customary library preparation involving gel measurement alternatives after every enzymatic manipulation, and (2) A modified PAR-CLIP process (“on-beads” PAR-CLIP), the place most RNA manipulations together with the required adapter ligation steps are carried out on the immobilized RNP. This streamlined process reduces the protocol preparation time by three days in comparison with the usual workflow.

Reverse genetics in excessive throughput: fast era of full adverse strand RNA virus cDNA clones and recombinant viruses thereof.

Reverse genetics approaches are indispensable instruments for proof of ideas in virus replication and pathogenesis. For adverse strand RNA viruses (NSVs) the restricted variety of infectious cDNA clones represents a bottleneck as clones are sometimes generated from cell tradition tailored or attenuated viruses, with restricted potential for pathogenesis analysis.

We developed a system through which cDNA copies of full NSV genomes have been immediately cloned into reverse genetics vectors by linear-to-linear RedE/T recombination. Fast cloning of a number of rabies virus (RABV) full size genomes and identification of clones an identical to area virus consensus sequence confirmed the approache’s reliability.

Recombinant viruses have been recovered from area virus cDNA clones. Related progress kinetics of parental and recombinant viruses, preservation of area virus characters in cell sort particular replication and virulence within the mouse mannequin have been confirmed. Diminished titers after reporter gene insertion indicated that the low stage of area virus replication is affected by gene insertions.

Genorise® Human ANP cDNA Clone, 10 ug
GR154008	Genorise Scientific	10 µg	EUR 153

Genorise® Human CRP cDNA Clone, 10 ug
GR154011	Genorise Scientific	10 µg	EUR 153

Genorise® Human cTnT cDNA Clone, 10 ug
GR154003	Genorise Scientific	10 µg	EUR 153

Genorise® Human TNN3 cDNA Clone, 10 ug
GR154004	Genorise Scientific	10 µg	EUR 153

Genorise® Human GPBB cDNA Clone, 10 ug
GR154010	Genorise Scientific	10 µg	EUR 153

Genorise® Human GLUT1 cDNA Clone, 10 ug
GR154002	Genorise Scientific	10 µg	EUR 153

Genorise® Human MyBPC3 cDNA Clone, 10 ug
GR154012	Genorise Scientific	10 µg	EUR 153

Genorise® Human Myoglobin cDNA Clone, 10 ug
GR154006	Genorise Scientific	10 µg	EUR 153

Genorise® Sheep IL-6 cDNA Clone, 10 ug
GR151001	Genorise Scientific	10 µg	EUR 153

Genorise® Ovine IL-2 cDNA Clone, 10 ug
GR151002	Genorise Scientific	10 µg	EUR 153

Genorise® Ovine IL-4 cDNA Clone, 10 ug
GR151003	Genorise Scientific	10 µg	EUR 153

Genorise® Canine IL-7 cDNA Clone, 10 ug
GR159002	Genorise Scientific	10 µg	EUR 153

Genorise® Bovine IL-1b cDNA Clone, 10 ug
GR152001	Genorise Scientific	10 µg	EUR 153

Genorise® Rabbit IL-1b cDNA Clone, 10 ug
GR153001	Genorise Scientific	10 µg	EUR 153

Genorise® Human H-FABP cDNA Clone, 10 ug
GR154001	Genorise Scientific	10 µg	EUR 153

Genorise® Human PAPP-A cDNA Clone, 10 ug
GR154013	Genorise Scientific	10 µg	EUR 153

Genorise® Human Pro-C3 cDNA Clone, 10 ug
GR154015	Genorise Scientific	10 µg	EUR 153

Genorise® Canine NGF-B cDNA Clone, 10 ug
GR159001	Genorise Scientific	10 µg	EUR 153

Genorise® Human Lp-PLA2 cDNA Clone, 10 ug
GR154007	Genorise Scientific	10 µg	EUR 153

Genorise® Human Myeloperoxidase cDNA Clone, 10 ug
GR154009	Genorise Scientific	10 µg	EUR 153

Genorise® Human NT-proBNP cDNA Clone, 10 ug
GR154014	Genorise Scientific	10 µg	EUR 153

pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]
CD510VB-1	SBI	>1 x 10^6 IFUs	EUR 720

pCDF1-MCS1 cDNA Cloning and Expression Vector
CD100A-1	SBI	10 ug	EUR 483

pCDH-CMV-MCS cDNA Cloning and Expression Vector
CD500B-1	SBI	10 ug	EUR 483

pCDH-EF1-MCS cDNA Cloning and Expression Vector
CD502A-1	SBI	10 ug	EUR 483

pCDH-CMV-MCS2 cDNA Cloning and Expression Vector
CD501A-1	SBI	10 ug	EUR 483

cDNA - Plant Normal Tissue: cDNA - Plant: Corn
C1634330	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Rice
C1634360	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Wheat
C1634390	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Orange
C1634340	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Potato
C1634350	Biochain	40 reactions	EUR 674

pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector
CD111B-1	SBI	10 ug	EUR 572

cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis
C1634310	Biochain	40 reactions	EUR 674

PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector
PB511B-1	SBI	10 ug	EUR 638

PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector
PB512B-1	SBI	10 ug	EUR 638

pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector
CD110B-1	SBI	10 ug	EUR 572

PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector
PB510B-1	SBI	10 ug	EUR 638

PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector
PB530A-2	SBI	10 ug	EUR 638

PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
PB531A-2	SBI	10 ug	EUR 638

PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector
PB533A-2	SBI	10 ug	EUR 638

cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean
C1634370	Biochain	40 reactions	EUR 674

pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector
CD512B-1	SBI	10 ug	EUR 572

pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector
CD514B-1	SBI	10 ug	EUR 572

pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector
CD510B-1	SBI	10 ug	EUR 572

pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector
CD530A-2	SBI	10 ug	EUR 590

pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
CD531A-2	SBI	10 ug	EUR 590

pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector
CD533A-2	SBI	10 ug	EUR 590

pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector
CD630A-1	SBI	10 ug	EUR 590

pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector
CD711B-1	SBI	10 ug	EUR 572

pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector
CD515B-1	SBI	10 ug	EUR 572

pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector
CD522A-1	SBI	10 ug	EUR 590

pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector
CD615B-1	SBI	10 ug	EUR 572

pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector
CD532A-2	SBI	10 ug	EUR 590

pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector
CD710B-1	SBI	10 ug	EUR 572

pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector
CD731B-1	SBI	10 ug	EUR 590

PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector
PB514B-2	SBI	10 ug	EUR 638

pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector
CD511B-1	SBI	10 ug	EUR 572

pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector
CD524A-1	SBI	10 ug	EUR 590

PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector
PB513B-1	SBI	10 ug	EUR 638

pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged]
CD512VA-1	SBI	>1 x 10^6 IFUs	EUR 720

PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
PB713B-1	SBI	10 ug	EUR 638

pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
CD513B-1	SBI	10 ug	EUR 572

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector
CD516B-2	SBI	10 ug	EUR 572

Novo? cDNA Kit
M1165-100	Biovision	each	EUR 424.8

Novo? cDNA Kit
M1165-25	Biovision	each	EUR 320.4

Recombinant Angiogenin (ANG)
4-RPA007Mu01	Cloud-Clone	EUR 573.73 EUR 277.20 EUR 1821.50 EUR 687.17 EUR 1254.34 EUR 459.60 EUR 4373.76	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)
4-RPA007Mu02	Cloud-Clone	EUR 573.73 EUR 277.20 EUR 1821.50 EUR 687.17 EUR 1254.34 EUR 459.60 EUR 4373.76	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)
4-RPA007Po01	Cloud-Clone	EUR 636.10 EUR 294.00 EUR 2055.36 EUR 765.12 EUR 1410.24 EUR 501.60 EUR 4958.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Pig Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)
4-RPA007Ra01	Cloud-Clone	EUR 582.34 EUR 279.60 EUR 1853.76 EUR 697.92 EUR 1275.84 EUR 465.60 EUR 4454.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)
4-RPA007Bo01	Cloud-Clone	EUR 605.99 EUR 285.60 EUR 1942.46 EUR 727.49 EUR 1334.98 EUR 481.20 EUR 4676.16	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Bovine Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)
4-RPA007Hu01	Cloud-Clone	EUR 550.08 EUR 271.20 EUR 1732.80 EUR 657.60 EUR 1195.20 EUR 444.00 EUR 4152.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Angiogenin expressed in: E.coli

pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector
CD713B-1	SBI	10 ug	EUR 572

Recombinant Angiostatin (ANG)
4-RPB254Hu01	Cloud-Clone	EUR 496.32 EUR 256.80 EUR 1531.20 EUR 590.40 EUR 1060.80 EUR 408.00 EUR 3648.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Angiostatin expressed in: E.coli

Recombinant Angiostatin (ANG)
4-RPB254Mu01	Cloud-Clone	EUR 496.32 EUR 256.80 EUR 1531.20 EUR 590.40 EUR 1060.80 EUR 408.00 EUR 3648.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Angiostatin expressed in: E.coli

Recombinant Angiostatin (ANG)
4-RPB254Ra01	Cloud-Clone	EUR 550.08 EUR 271.20 EUR 1732.80 EUR 657.60 EUR 1195.20 EUR 444.00 EUR 4152.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Angiostatin expressed in: E.coli

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged]
CD516VB-2	SBI	>1 x 10^6 IFUs	EUR 706

cDNA - Lupus: Lung
C1236152Lup	Biochain	40 reactions	EUR 737

pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg
CD527A-1	SBI	10 ug	EUR 590

cDNA - Lupus: Colon
C1236090Lup	Biochain	40 reactions	EUR 737

cDNA - Lupus: Heart
C1236122Lup	Biochain	40 reactions	EUR 737

cDNA - Lupus: Liver
C1236149Lup	Biochain	40 reactions	EUR 737

cDNA - Asthma: Lung
C1236152Ld-1	Biochain	40 reactions	EUR 899

cDNA - Lupus: Kidney
C1236142Lup	Biochain	40 reactions	EUR 737

cDNA - Lupus: Spleen
C1236246Lup	Biochain	40 reactions	EUR 737

Evo? cDNA Supermix
M1168-100	Biovision	each	EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision	each	EUR 320.4

pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged]
CD513VB-1	SBI	>1 x 10^6 IFUs	EUR 720

cDNA - Lupus: stomach
C1236248Lup	Biochain	40 reactions	EUR 737

Novo? cDNA Supermix
M1169-100	Biovision	each	EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision	each	EUR 346.8

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus]
CD511VB-1	SBI	>1 x 10^6 IFUs	EUR 720

cDNA - Emphysema: Lung
C1236152Ld-3	Biochain	40 reactions	EUR 899

cDNA - Pneumonia: Lung
C1236152Ld-4	Biochain	40 reactions	EUR 899

cDNA - Lupus: Pancreas
C1236188Lup	Biochain	40 reactions	EUR 737

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems	Abbexa	EUR 744.00 EUR 627.60	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)
20-abx09802120ulSystems	Abbexa	EUR 1045.20 EUR 794.40	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

cDNA - Bronchitis: Lung
C1236152Ld-2	Biochain	40 reactions	EUR 899

cDNA - hypertension: Vein
C1236020Hd-2	Biochain	40 reactions	EUR 729

Monoclonal GOK / STIM1 Antibody (C-Terminus, clone CDN3H4), Clone: CDN3H4
AMM05163G	Leading Biology	0.05mg	EUR 580.8
Description: A Monoclonal antibody against Human GOK / STIM1 (C-Terminus, clone CDN3H4). The antibodies are raised in Mouse and are from clone CDN3H4. This antibody is applicable in WB and IHC-P, ICC, IP

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	EUR 678.00 EUR 577.20	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

cDNA - hypertension: Heart
C1236122Hd-2	Biochain	40 reactions	EUR 729

Evo™ cDNA Kit
M1164-100	Biovision	each	Ask for price

Evo™ cDNA Kit
M1164-25	Biovision	each	Ask for price

cDNA - hypertension: Artery
C1236013Hd-2	Biochain	40 reactions	EUR 729

cDNA - hypertension: Kidney
C1236142Hd-2	Biochain	40 reactions	EUR 729

cDNA - Arteriosclerosis: Aorta
C1236012Hd-4	Biochain	40 reactions	EUR 899

cDNA - Arteriosclerosis: Artery
C1236013Hd-4	Biochain	40 reactions	EUR 899

pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector
CD550A-1	SBI	10 ug	EUR 563

Human eNOS cDNA probe
eNOS51-D-2	Alpha Diagnostics	2 ug	EUR 534

cDNA - Lupus: Spinal Cord
C1236234Lup	Biochain	40 reactions	EUR 737

PB-CuO-CMV-MCS-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector
PBQM800A-1	SBI	10 µg	EUR 921

Pig Angiogenin (ANG) ELISA Kit
SEA007Po-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 6117.62
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Angiogenin (ANG) in serum, plasma and other biological fluids.

Pig Angiogenin (ANG) ELISA Kit
SEA007Po-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 608.98
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Angiogenin (ANG) in serum, plasma and other biological fluids.

Pig Angiogenin (ANG) ELISA Kit
SEA007Po-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 818.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Angiogenin (ANG) in serum, plasma and other biological fluids.

Pig Angiogenin (ANG) ELISA Kit
SEA007Po-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3323.45
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Angiogenin (ANG) in serum, plasma and other biological fluids.

Pig Angiogenin (ANG) ELISA Kit
4-SEA007Po	Cloud-Clone	EUR 6178.80 EUR 3264.00 EUR 819.60	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Angiogenin (ANG) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.

Rat Angiostatin (ANG) ELISA Kit
SEB254Ra-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5850.59
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiostatin (ANG) in serum, plasma, tissue homogenates and other biological fluids.

Rat Angiostatin (ANG) ELISA Kit
SEB254Ra-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 586.99
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiostatin (ANG) in serum, plasma, tissue homogenates and other biological fluids.

Rat Angiostatin (ANG) ELISA Kit
SEB254Ra-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 787.13
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiostatin (ANG) in serum, plasma, tissue homogenates and other biological fluids.

Rat Angiostatin (ANG) ELISA Kit
SEB254Ra-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3182.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiostatin (ANG) in serum, plasma, tissue homogenates and other biological fluids.

Rat Angiostatin (ANG) ELISA Kit
4-SEB254Ra	Cloud-Clone	EUR 5911.20 EUR 3122.40 EUR 787.20	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Angiostatin (ANG) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Tetro cDNA Synthesis Kit
BIO-65042	Bioline	30 Reactions	Ask for price

Tetro cDNA Synthesis Kit
BIO-65043	Bioline	100 Reactions	Ask for price

EVery cDNA Synthesis Kit
EVery200B-1	SBI	20 preps	EUR 307

Human Angiogenin (ANG) ELISA Kit
SEA007Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4091.29
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Angiogenin (ANG) ELISA Kit
SEA007Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 442.1
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Angiogenin (ANG) ELISA Kit
SEA007Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 580.15
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Angiogenin (ANG) ELISA Kit
SEA007Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2250.68
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Angiogenin (ANG) ELISA Kit
4-SEA007Hu	Cloud-Clone	EUR 4152.00 EUR 2191.20 EUR 580.80	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Angiogenin (ANG) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Angiogenin (ANG) ELISA Kit
SEA007Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5269.39
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiogenin (ANG) in serum, plasma and other biological fluids.

Mouse Angiogenin (ANG) ELISA Kit
SEA007Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 539.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiogenin (ANG) in serum, plasma and other biological fluids.

Mouse Angiogenin (ANG) ELISA Kit
SEA007Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 718.75
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiogenin (ANG) in serum, plasma and other biological fluids.

Mouse Angiogenin (ANG) ELISA Kit
SEA007Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2874.38
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiogenin (ANG) in serum, plasma and other biological fluids.

Mouse Angiogenin (ANG) ELISA Kit
4-SEA007Mu	Cloud-Clone	EUR 5330.40 EUR 2815.20 EUR 718.80	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Angiogenin (ANG) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Cattle Angiogenin (ANG) ELISA Kit
SEA007Bo-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 6117.62
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Angiogenin (ANG) in serum, plasma and other biological fluids.

Cattle Angiogenin (ANG) ELISA Kit
SEA007Bo-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 608.98
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Angiogenin (ANG) in serum, plasma and other biological fluids.

Cattle Angiogenin (ANG) ELISA Kit
SEA007Bo-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 818.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Angiogenin (ANG) in serum, plasma and other biological fluids.

Cattle Angiogenin (ANG) ELISA Kit
SEA007Bo-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3323.45
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Angiogenin (ANG) in serum, plasma and other biological fluids.

Cattle Angiogenin (ANG) ELISA Kit
4-SEA007Bo	Cloud-Clone	EUR 6178.80 EUR 3264.00 EUR 819.60	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Angiogenin (ANG) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.

Human Angiostatin (ANG) ELISA Kit
SEB254Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5402.92
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiostatin (ANG) in serum, plasma, urine and other biological fluids.

Human Angiostatin (ANG) ELISA Kit
SEB254Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 550.13
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiostatin (ANG) in serum, plasma, urine and other biological fluids.

Human Angiostatin (ANG) ELISA Kit
SEB254Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 734.46
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiostatin (ANG) in serum, plasma, urine and other biological fluids.

Human Angiostatin (ANG) ELISA Kit
SEB254Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2945.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiostatin (ANG) in serum, plasma, urine and other biological fluids.

Human Angiostatin (ANG) ELISA Kit
4-SEB254Hu	Cloud-Clone	EUR 5463.60 EUR 2886.00 EUR 735.60	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Angiostatin (ANG) in samples from Serum, plasma, urine and other biological fluids. with no significant corss-reactivity with analogues from other species.

Mouse Angiostatin (ANG) ELISA Kit
SEB254Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5552.14
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiostatin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Angiostatin (ANG) ELISA Kit
SEB254Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 562.42
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiostatin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Angiostatin (ANG) ELISA Kit
SEB254Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 752.02
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiostatin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Angiostatin (ANG) ELISA Kit
SEB254Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3024.07
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiostatin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Angiostatin (ANG) ELISA Kit
4-SEB254Mu	Cloud-Clone	EUR 5612.40 EUR 2965.20 EUR 752.40	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Angiostatin (ANG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

cDNA - Liver Cirrhosis: Lung
C1236152Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Skin
C1236218Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Brain
C1236035Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Colon
C1236090Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Heart
C1236122Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Liver
C1236149Lcs	Biochain	40 reactions	EUR 729

cDNA - Lupus: Small Intestine
C1236226Lup	Biochain	40 reactions	EUR 737

circRNA cDNA Synthesis Kit
G627	ABM	25 rxn (20 ul/rxn)	EUR 370.8

Angiogenin (ANG) Polyclonal Antibody (Pig)
4-PAA007Po01	Cloud-Clone	EUR 310.80 EUR 3232.80 EUR 800.40 EUR 391.20 EUR 261.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Pig Angiogenin (ANG)

Angiogenin (ANG) Polyclonal Antibody (Rat)
4-PAA007Ra01	Cloud-Clone	EUR 291.60 EUR 2948.40 EUR 735.60 EUR 366.00 EUR 254.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Angiogenin (ANG)

Angiostatin (ANG) Polyclonal Antibody (Rat)
4-PAB254Ra01	Cloud-Clone	EUR 301.20 EUR 3091.20 EUR 768.00 EUR 379.20 EUR 258.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Angiostatin (ANG)

Angiogenin (ANG) Polyclonal Antibody (Human)
4-PAA007Hu01	Cloud-Clone	EUR 232.80 EUR 2013.60 EUR 523.20 EUR 280.80 EUR 229.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Angiogenin (ANG)

Angiogenin (ANG) Polyclonal Antibody (Mouse)
4-PAA007Mu01	Cloud-Clone	EUR 283.20 EUR 2805.60 EUR 703.20 EUR 352.80 EUR 250.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Angiogenin (ANG)

Angiogenin (ANG) Polyclonal Antibody (Mouse)
4-PAA007Mu02	Cloud-Clone	EUR 283.20 EUR 2805.60 EUR 703.20 EUR 352.80 EUR 250.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Angiogenin (ANG)

Angiogenin (ANG) Monoclonal Antibody (Human)
4-MAA007Hu22	Cloud-Clone	EUR 298.80 EUR 3058.80 EUR 760.80 EUR 375.60 EUR 258.00	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Angiogenin (ANG)

cDNA Probe Diluent Solution
AR0063	BosterBio	5mL	EUR 127.2

cDNA - Liver Cirrhosis: Kidney
C1236142Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Spleen
C1236246Lcs	Biochain	40 reactions	EUR 729

Novo? Transcriptome cDNA Kit
M1167-100	Biovision	each	EUR 1142.4

Novo? Transcriptome cDNA Kit
M1167-25	Biovision	each	EUR 529.2

Angiogenin (ANG) Polyclonal Antibody (Bovine)
4-PAA007Bo01	Cloud-Clone	EUR 310.80 EUR 3232.80 EUR 800.40 EUR 391.20 EUR 261.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Angiogenin (ANG)

Angiostatin (ANG) Polyclonal Antibody (Mouse)
4-PAB254Mu01	Cloud-Clone	EUR 291.60 EUR 2940.00 EUR 733.20 EUR 364.80 EUR 254.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Angiostatin (ANG)

Angiogenin (ANG) Monoclonal Antibody (Bovine)
4-MAA007Bo22	Cloud-Clone	EUR 320.40 EUR 3391.20 EUR 836.40 EUR 405.60 EUR 266.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Bovine Angiogenin (ANG)

PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector
PBQM812A-1	SBI	10 ug	EUR 1023

SensiFAST cDNA Synthesis Kit
BIO-65053	Bioline	50 Reactions	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053/S	Bioline	Sample	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65054	Bioline	250 Reactions	Ask for price

The pliability of the technique was demonstrated by cloning a number of copies of an orthobunyavirus L genome phase. This necessary step in reverse genetics expertise improvement opens novel avenues for the evaluation of virus variability mixed with phenotypical characterization of recombinant viruses at a clonal stage.