A number of biosafety stage Three and/or 4 (BSL-3/4) pathogens are high-consequence, single-stranded RNA viruses, and their genomes, when launched into permissive cells, are infectious. Furthermore, many of those viruses are choose brokers (SAs), and their genomes are additionally thought-about SAs. Because of this, cDNAs and/or their derivatives have to be examined to make sure the absence of infectious virus and/or viral RNA earlier than switch out of the BSL-3/Four and/or SA laboratory.
This tremendously limits the capability to conduct viral genomic analysis, significantly the appliance of next-generation sequencing (NGS). Right here, we current a sequence-independent methodology to quickly amplify viral genomic RNA whereas concurrently abolishing each viral and genomic RNA infectivity throughout a number of single-stranded positive-sense RNA (ssRNA+) virus households.
The method generates barcoded DNA amplicons that vary in size from 300 to 1,000 bp, which can’t be used to rescue a virus and are secure to move at room temperature. Our barcoding method permits for as much as 288 barcoded samples to be pooled right into a single library and run throughout varied NGS platforms with out potential reconstitution of the viral genome.
Our information display that this method gives full-length genomic sequence data not solely from high-titer virion preparations however it will possibly additionally get better particular viral sequence from samples with restricted beginning materials within the background of mobile RNA, and it may be used to determine pathogens from unknown samples.
In abstract, we describe a fast, common customary working process that generates high-quality NGS libraries freed from infectious virus and infectious viral RNA. IMPORTANCE This report establishes and validates a typical working process (SOP) for choose brokers (SAs) and different biosafety stage Three and/or 4 (BSL-3/4) RNA viruses to quickly generate noninfectious, barcoded cDNA amenable for next-generation sequencing (NGS).
This eliminates the burden of testing all processed samples derived from high-consequence pathogens previous to switch from high-containment laboratories to lower-containment services for sequencing. Our established protocol might be scaled up for high-throughput sequencing of tons of of samples concurrently, which might dramatically scale back the associated fee and energy required for NGS library building. NGS information from this SOP can present full genome protection from viral shares and also can detect virus-specific reads from restricted beginning materials. Our information counsel that the process might be carried out and simply validated by institutional biosafety committees throughout analysis laboratories.
PAR-CLIP and streamlined small RNA cDNA library preparation protocol for the identification of RNA binding protein goal websites.
The research of protein-RNA interactions is essential for our understanding of mobile processes and regulatory circuits managed by RNA binding proteins (RBPs). Latest subsequent era sequencing-based approaches considerably promoted our understanding of RNA biology and its significance for cell operate. We current a streamlined protocol for Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP), a method that permits for the characterization of RBP binding websites on the right track RNAs at nucleotide decision and transcriptome-wide scale.

PAR-CLIP entails irreversible UV-mediated crosslinking of RNAs labeled with photoreactive nucleosides to interacting proteins, adopted by stringent purification steps and the conversion of crosslinked RNA into small RNA cDNA libraries suitable with next-generation sequencing. The defining hallmark of PAR-CLIP is a diagnostic mutation on the crosslinking website that’s launched into cDNA in the course of the library preparation course of. This characteristic permits for environment friendly computational removing of contaminating sequences derived from non-crosslinked fragments of ample mobile RNAs.
Within the following, we current two completely different step-by-step procedures for PAR-CLIP, which differ within the small RNA cDNA library preparation process: (1) Customary library preparation involving gel measurement alternatives after every enzymatic manipulation, and (2) A modified PAR-CLIP process (“on-beads” PAR-CLIP), the place most RNA manipulations together with the required adapter ligation steps are carried out on the immobilized RNP. This streamlined process reduces the protocol preparation time by three days in comparison with the usual workflow.
Reverse genetics in excessive throughput: fast era of full adverse strand RNA virus cDNA clones and recombinant viruses thereof.
Reverse genetics approaches are indispensable instruments for proof of ideas in virus replication and pathogenesis. For adverse strand RNA viruses (NSVs) the restricted variety of infectious cDNA clones represents a bottleneck as clones are sometimes generated from cell tradition tailored or attenuated viruses, with restricted potential for pathogenesis analysis.
We developed a system through which cDNA copies of full NSV genomes have been immediately cloned into reverse genetics vectors by linear-to-linear RedE/T recombination. Fast cloning of a number of rabies virus (RABV) full size genomes and identification of clones an identical to area virus consensus sequence confirmed the approache’s reliability.
Recombinant viruses have been recovered from area virus cDNA clones. Related progress kinetics of parental and recombinant viruses, preservation of area virus characters in cell sort particular replication and virulence within the mouse mannequin have been confirmed. Diminished titers after reporter gene insertion indicated that the low stage of area virus replication is affected by gene insertions.
pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus] |
CD510VB-1 |
SBI |
>1 x 10^6 IFUs |
EUR 720 |
pCDF1-MCS1 cDNA Cloning and Expression Vector |
CD100A-1 |
SBI |
10 ug |
EUR 483 |
pCDH-CMV-MCS cDNA Cloning and Expression Vector |
CD500B-1 |
SBI |
10 ug |
EUR 483 |
pCDH-EF1-MCS cDNA Cloning and Expression Vector |
CD502A-1 |
SBI |
10 ug |
EUR 483 |
pCDH-CMV-MCS2 cDNA Cloning and Expression Vector |
CD501A-1 |
SBI |
10 ug |
EUR 483 |
cDNA - Plant Normal Tissue: cDNA - Plant: Corn |
C1634330 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Rice |
C1634360 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Wheat |
C1634390 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Orange |
C1634340 |
Biochain |
40 reactions |
EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Potato |
C1634350 |
Biochain |
40 reactions |
EUR 674 |
pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector |
CD111B-1 |
SBI |
10 ug |
EUR 572 |
cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis |
C1634310 |
Biochain |
40 reactions |
EUR 674 |
PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector |
PB511B-1 |
SBI |
10 ug |
EUR 638 |
PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector |
PB512B-1 |
SBI |
10 ug |
EUR 638 |
pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector |
CD110B-1 |
SBI |
10 ug |
EUR 572 |
PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector |
PB510B-1 |
SBI |
10 ug |
EUR 638 |
PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector |
PB530A-2 |
SBI |
10 ug |
EUR 638 |
PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
PB531A-2 |
SBI |
10 ug |
EUR 638 |
PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector |
PB533A-2 |
SBI |
10 ug |
EUR 638 |
cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean |
C1634370 |
Biochain |
40 reactions |
EUR 674 |
pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector |
CD512B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector |
CD514B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
CD510B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector |
CD530A-2 |
SBI |
10 ug |
EUR 590 |
pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
CD531A-2 |
SBI |
10 ug |
EUR 590 |
pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector |
CD533A-2 |
SBI |
10 ug |
EUR 590 |
pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector |
CD630A-1 |
SBI |
10 ug |
EUR 590 |
pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector |
CD711B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
CD515B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector |
CD522A-1 |
SBI |
10 ug |
EUR 590 |
pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
CD615B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector |
CD532A-2 |
SBI |
10 ug |
EUR 590 |
pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
CD710B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector |
CD731B-1 |
SBI |
10 ug |
EUR 590 |
PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector |
PB514B-2 |
SBI |
10 ug |
EUR 638 |
pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector |
CD511B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector |
CD524A-1 |
SBI |
10 ug |
EUR 590 |
PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector |
PB513B-1 |
SBI |
10 ug |
EUR 638 |
pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged] |
CD512VA-1 |
SBI |
>1 x 10^6 IFUs |
EUR 720 |
PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
PB713B-1 |
SBI |
10 ug |
EUR 638 |
pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
CD513B-1 |
SBI |
10 ug |
EUR 572 |
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector |
CD516B-2 |
SBI |
10 ug |
EUR 572 |
Novo? cDNA Kit |
M1165-100 |
Biovision |
each |
EUR 424.8 |
Novo? cDNA Kit |
M1165-25 |
Biovision |
each |
EUR 320.4 |
Recombinant Angiogenin (ANG) |
4-RPA007Mu01 |
Cloud-Clone |
-
EUR 573.73
-
EUR 277.20
-
EUR 1821.50
-
EUR 687.17
-
EUR 1254.34
-
EUR 459.60
-
EUR 4373.76
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Angiogenin expressed in: E.coli |
Recombinant Angiogenin (ANG) |
4-RPA007Mu02 |
Cloud-Clone |
-
EUR 573.73
-
EUR 277.20
-
EUR 1821.50
-
EUR 687.17
-
EUR 1254.34
-
EUR 459.60
-
EUR 4373.76
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Angiogenin expressed in: E.coli |
Recombinant Angiogenin (ANG) |
4-RPA007Po01 |
Cloud-Clone |
-
EUR 636.10
-
EUR 294.00
-
EUR 2055.36
-
EUR 765.12
-
EUR 1410.24
-
EUR 501.60
-
EUR 4958.40
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Pig Angiogenin expressed in: E.coli |
Recombinant Angiogenin (ANG) |
4-RPA007Ra01 |
Cloud-Clone |
-
EUR 582.34
-
EUR 279.60
-
EUR 1853.76
-
EUR 697.92
-
EUR 1275.84
-
EUR 465.60
-
EUR 4454.40
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Rat Angiogenin expressed in: E.coli |
Recombinant Angiogenin (ANG) |
4-RPA007Bo01 |
Cloud-Clone |
-
EUR 605.99
-
EUR 285.60
-
EUR 1942.46
-
EUR 727.49
-
EUR 1334.98
-
EUR 481.20
-
EUR 4676.16
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Bovine Angiogenin expressed in: E.coli |
Recombinant Angiogenin (ANG) |
4-RPA007Hu01 |
Cloud-Clone |
-
EUR 550.08
-
EUR 271.20
-
EUR 1732.80
-
EUR 657.60
-
EUR 1195.20
-
EUR 444.00
-
EUR 4152.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Angiogenin expressed in: E.coli |
pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector |
CD713B-1 |
SBI |
10 ug |
EUR 572 |
Recombinant Angiostatin (ANG) |
4-RPB254Hu01 |
Cloud-Clone |
-
EUR 496.32
-
EUR 256.80
-
EUR 1531.20
-
EUR 590.40
-
EUR 1060.80
-
EUR 408.00
-
EUR 3648.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Angiostatin expressed in: E.coli |
Recombinant Angiostatin (ANG) |
4-RPB254Mu01 |
Cloud-Clone |
-
EUR 496.32
-
EUR 256.80
-
EUR 1531.20
-
EUR 590.40
-
EUR 1060.80
-
EUR 408.00
-
EUR 3648.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Angiostatin expressed in: E.coli |
Recombinant Angiostatin (ANG) |
4-RPB254Ra01 |
Cloud-Clone |
-
EUR 550.08
-
EUR 271.20
-
EUR 1732.80
-
EUR 657.60
-
EUR 1195.20
-
EUR 444.00
-
EUR 4152.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Rat Angiostatin expressed in: E.coli |
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged] |
CD516VB-2 |
SBI |
>1 x 10^6 IFUs |
EUR 706 |
cDNA - Lupus: Lung |
C1236152Lup |
Biochain |
40 reactions |
EUR 737 |
pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg |
CD527A-1 |
SBI |
10 ug |
EUR 590 |
cDNA - Lupus: Colon |
C1236090Lup |
Biochain |
40 reactions |
EUR 737 |
cDNA - Lupus: Heart |
C1236122Lup |
Biochain |
40 reactions |
EUR 737 |
cDNA - Lupus: Liver |
C1236149Lup |
Biochain |
40 reactions |
EUR 737 |
cDNA - Asthma: Lung |
C1236152Ld-1 |
Biochain |
40 reactions |
EUR 899 |
cDNA - Lupus: Kidney |
C1236142Lup |
Biochain |
40 reactions |
EUR 737 |
cDNA - Lupus: Spleen |
C1236246Lup |
Biochain |
40 reactions |
EUR 737 |
Evo? cDNA Supermix |
M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
each |
EUR 320.4 |
pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged] |
CD513VB-1 |
SBI |
>1 x 10^6 IFUs |
EUR 720 |
cDNA - Lupus: stomach |
C1236248Lup |
Biochain |
40 reactions |
EUR 737 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
each |
EUR 346.8 |
pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus] |
CD511VB-1 |
SBI |
>1 x 10^6 IFUs |
EUR 720 |
cDNA - Emphysema: Lung |
C1236152Ld-3 |
Biochain |
40 reactions |
EUR 899 |
cDNA - Pneumonia: Lung |
C1236152Ld-4 |
Biochain |
40 reactions |
EUR 899 |
cDNA - Lupus: Pancreas |
C1236188Lup |
Biochain |
40 reactions |
EUR 737 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
20-abx09801620ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
20-abx09802120ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
cDNA - Bronchitis: Lung |
C1236152Ld-2 |
Biochain |
40 reactions |
EUR 899 |
cDNA - hypertension: Vein |
C1236020Hd-2 |
Biochain |
40 reactions |
EUR 729 |
Monoclonal GOK / STIM1 Antibody (C-Terminus, clone CDN3H4), Clone: CDN3H4 |
AMM05163G |
Leading Biology |
0.05mg |
EUR 580.8 |
Description: A Monoclonal antibody against Human GOK / STIM1 (C-Terminus, clone CDN3H4). The antibodies are raised in Mouse and are from clone CDN3H4. This antibody is applicable in WB and IHC-P, ICC, IP |
cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
cDNA - hypertension: Heart |
C1236122Hd-2 |
Biochain |
40 reactions |
EUR 729 |
Evo™ cDNA Kit |
M1164-100 |
Biovision |
each |
Ask for price |
Evo™ cDNA Kit |
M1164-25 |
Biovision |
each |
Ask for price |
cDNA - hypertension: Artery |
C1236013Hd-2 |
Biochain |
40 reactions |
EUR 729 |
cDNA - hypertension: Kidney |
C1236142Hd-2 |
Biochain |
40 reactions |
EUR 729 |
cDNA - Arteriosclerosis: Aorta |
C1236012Hd-4 |
Biochain |
40 reactions |
EUR 899 |
cDNA - Arteriosclerosis: Artery |
C1236013Hd-4 |
Biochain |
40 reactions |
EUR 899 |
pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector |
CD550A-1 |
SBI |
10 ug |
EUR 563 |
cDNA - Lupus: Spinal Cord |
C1236234Lup |
Biochain |
40 reactions |
EUR 737 |
PB-CuO-CMV-MCS-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector |
PBQM800A-1 |
SBI |
10 µg |
EUR 921 |
Pig Angiogenin (ANG) ELISA Kit |
SEA007Po-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 6117.62 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Angiogenin (ANG) in serum, plasma and other biological fluids. |
Pig Angiogenin (ANG) ELISA Kit |
SEA007Po-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 608.98 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Angiogenin (ANG) in serum, plasma and other biological fluids. |
Pig Angiogenin (ANG) ELISA Kit |
SEA007Po-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 818.54 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Angiogenin (ANG) in serum, plasma and other biological fluids. |
Pig Angiogenin (ANG) ELISA Kit |
SEA007Po-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3323.45 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Angiogenin (ANG) in serum, plasma and other biological fluids. |
Pig Angiogenin (ANG) ELISA Kit |
4-SEA007Po |
Cloud-Clone |
-
EUR 6178.80
-
EUR 3264.00
-
EUR 819.60
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Angiogenin (ANG) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Rat Angiostatin (ANG) ELISA Kit |
SEB254Ra-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5850.59 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiostatin (ANG) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Angiostatin (ANG) ELISA Kit |
SEB254Ra-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 586.99 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiostatin (ANG) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Angiostatin (ANG) ELISA Kit |
SEB254Ra-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 787.13 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiostatin (ANG) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Angiostatin (ANG) ELISA Kit |
SEB254Ra-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3182.08 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiostatin (ANG) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Angiostatin (ANG) ELISA Kit |
4-SEB254Ra |
Cloud-Clone |
-
EUR 5911.20
-
EUR 3122.40
-
EUR 787.20
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Angiostatin (ANG) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Tetro cDNA Synthesis Kit |
BIO-65042 |
Bioline |
30 Reactions |
Ask for price |
Tetro cDNA Synthesis Kit |
BIO-65043 |
Bioline |
100 Reactions |
Ask for price |
EVery cDNA Synthesis Kit |
EVery200B-1 |
SBI |
20 preps |
EUR 307 |
Human Angiogenin (ANG) ELISA Kit |
SEA007Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4091.29 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Angiogenin (ANG) ELISA Kit |
SEA007Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 442.1 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Angiogenin (ANG) ELISA Kit |
SEA007Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 580.15 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Angiogenin (ANG) ELISA Kit |
SEA007Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2250.68 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Angiogenin (ANG) ELISA Kit |
4-SEA007Hu |
Cloud-Clone |
-
EUR 4152.00
-
EUR 2191.20
-
EUR 580.80
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Angiogenin (ANG) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Mouse Angiogenin (ANG) ELISA Kit |
SEA007Mu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5269.39 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiogenin (ANG) in serum, plasma and other biological fluids. |
Mouse Angiogenin (ANG) ELISA Kit |
SEA007Mu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 539.12 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiogenin (ANG) in serum, plasma and other biological fluids. |
Mouse Angiogenin (ANG) ELISA Kit |
SEA007Mu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 718.75 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiogenin (ANG) in serum, plasma and other biological fluids. |
Mouse Angiogenin (ANG) ELISA Kit |
SEA007Mu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2874.38 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiogenin (ANG) in serum, plasma and other biological fluids. |
Mouse Angiogenin (ANG) ELISA Kit |
4-SEA007Mu |
Cloud-Clone |
-
EUR 5330.40
-
EUR 2815.20
-
EUR 718.80
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Angiogenin (ANG) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. |
Cattle Angiogenin (ANG) ELISA Kit |
SEA007Bo-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 6117.62 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Angiogenin (ANG) in serum, plasma and other biological fluids. |
Cattle Angiogenin (ANG) ELISA Kit |
SEA007Bo-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 608.98 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Angiogenin (ANG) in serum, plasma and other biological fluids. |
Cattle Angiogenin (ANG) ELISA Kit |
SEA007Bo-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 818.54 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Angiogenin (ANG) in serum, plasma and other biological fluids. |
Cattle Angiogenin (ANG) ELISA Kit |
SEA007Bo-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3323.45 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Angiogenin (ANG) in serum, plasma and other biological fluids. |
Cattle Angiogenin (ANG) ELISA Kit |
4-SEA007Bo |
Cloud-Clone |
-
EUR 6178.80
-
EUR 3264.00
-
EUR 819.60
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Angiogenin (ANG) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Human Angiostatin (ANG) ELISA Kit |
SEB254Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5402.92 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiostatin (ANG) in serum, plasma, urine and other biological fluids. |
Human Angiostatin (ANG) ELISA Kit |
SEB254Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 550.13 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiostatin (ANG) in serum, plasma, urine and other biological fluids. |
Human Angiostatin (ANG) ELISA Kit |
SEB254Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 734.46 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiostatin (ANG) in serum, plasma, urine and other biological fluids. |
Human Angiostatin (ANG) ELISA Kit |
SEB254Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2945.08 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiostatin (ANG) in serum, plasma, urine and other biological fluids. |
Human Angiostatin (ANG) ELISA Kit |
4-SEB254Hu |
Cloud-Clone |
-
EUR 5463.60
-
EUR 2886.00
-
EUR 735.60
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Angiostatin (ANG) in samples from Serum, plasma, urine and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Mouse Angiostatin (ANG) ELISA Kit |
SEB254Mu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5552.14 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiostatin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Mouse Angiostatin (ANG) ELISA Kit |
SEB254Mu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 562.42 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiostatin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Mouse Angiostatin (ANG) ELISA Kit |
SEB254Mu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 752.02 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiostatin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Mouse Angiostatin (ANG) ELISA Kit |
SEB254Mu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3024.07 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiostatin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Mouse Angiostatin (ANG) ELISA Kit |
4-SEB254Mu |
Cloud-Clone |
-
EUR 5612.40
-
EUR 2965.20
-
EUR 752.40
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Angiostatin (ANG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. |
cDNA - Liver Cirrhosis: Lung |
C1236152Lcs |
Biochain |
40 reactions |
EUR 729 |
cDNA - Liver Cirrhosis: Skin |
C1236218Lcs |
Biochain |
40 reactions |
EUR 729 |
cDNA - Liver Cirrhosis: Brain |
C1236035Lcs |
Biochain |
40 reactions |
EUR 729 |
cDNA - Liver Cirrhosis: Colon |
C1236090Lcs |
Biochain |
40 reactions |
EUR 729 |
cDNA - Liver Cirrhosis: Heart |
C1236122Lcs |
Biochain |
40 reactions |
EUR 729 |
cDNA - Liver Cirrhosis: Liver |
C1236149Lcs |
Biochain |
40 reactions |
EUR 729 |
cDNA - Lupus: Small Intestine |
C1236226Lup |
Biochain |
40 reactions |
EUR 737 |
circRNA cDNA Synthesis Kit |
G627 |
ABM |
25 rxn (20 ul/rxn) |
EUR 370.8 |
Angiogenin (ANG) Polyclonal Antibody (Pig) |
4-PAA007Po01 |
Cloud-Clone |
-
EUR 310.80
-
EUR 3232.80
-
EUR 800.40
-
EUR 391.20
-
EUR 261.60
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pig Angiogenin (ANG) |
Angiogenin (ANG) Polyclonal Antibody (Rat) |
4-PAA007Ra01 |
Cloud-Clone |
-
EUR 291.60
-
EUR 2948.40
-
EUR 735.60
-
EUR 366.00
-
EUR 254.40
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Rat Angiogenin (ANG) |
Angiostatin (ANG) Polyclonal Antibody (Rat) |
4-PAB254Ra01 |
Cloud-Clone |
-
EUR 301.20
-
EUR 3091.20
-
EUR 768.00
-
EUR 379.20
-
EUR 258.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Rat Angiostatin (ANG) |
Angiogenin (ANG) Polyclonal Antibody (Human) |
4-PAA007Hu01 |
Cloud-Clone |
-
EUR 232.80
-
EUR 2013.60
-
EUR 523.20
-
EUR 280.80
-
EUR 229.20
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Angiogenin (ANG) |
Angiogenin (ANG) Polyclonal Antibody (Mouse) |
4-PAA007Mu01 |
Cloud-Clone |
-
EUR 283.20
-
EUR 2805.60
-
EUR 703.20
-
EUR 352.80
-
EUR 250.80
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Angiogenin (ANG) |
Angiogenin (ANG) Polyclonal Antibody (Mouse) |
4-PAA007Mu02 |
Cloud-Clone |
-
EUR 283.20
-
EUR 2805.60
-
EUR 703.20
-
EUR 352.80
-
EUR 250.80
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Angiogenin (ANG) |
Angiogenin (ANG) Monoclonal Antibody (Human) |
4-MAA007Hu22 |
Cloud-Clone |
-
EUR 298.80
-
EUR 3058.80
-
EUR 760.80
-
EUR 375.60
-
EUR 258.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against Human Angiogenin (ANG) |
cDNA Probe Diluent Solution |
AR0063 |
BosterBio |
5mL |
EUR 127.2 |
cDNA - Liver Cirrhosis: Kidney |
C1236142Lcs |
Biochain |
40 reactions |
EUR 729 |
cDNA - Liver Cirrhosis: Spleen |
C1236246Lcs |
Biochain |
40 reactions |
EUR 729 |
Novo? Transcriptome cDNA Kit |
M1167-100 |
Biovision |
each |
EUR 1142.4 |
Novo? Transcriptome cDNA Kit |
M1167-25 |
Biovision |
each |
EUR 529.2 |
Angiogenin (ANG) Polyclonal Antibody (Bovine) |
4-PAA007Bo01 |
Cloud-Clone |
-
EUR 310.80
-
EUR 3232.80
-
EUR 800.40
-
EUR 391.20
-
EUR 261.60
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Bovine Angiogenin (ANG) |
Angiostatin (ANG) Polyclonal Antibody (Mouse) |
4-PAB254Mu01 |
Cloud-Clone |
-
EUR 291.60
-
EUR 2940.00
-
EUR 733.20
-
EUR 364.80
-
EUR 254.40
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Angiostatin (ANG) |
Angiogenin (ANG) Monoclonal Antibody (Bovine) |
4-MAA007Bo22 |
Cloud-Clone |
-
EUR 320.40
-
EUR 3391.20
-
EUR 836.40
-
EUR 405.60
-
EUR 266.40
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against Bovine Angiogenin (ANG) |
PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector |
PBQM812A-1 |
SBI |
10 ug |
EUR 1023 |
SensiFAST cDNA Synthesis Kit |
BIO-65053 |
Bioline |
50 Reactions |
Ask for price |
SensiFAST cDNA Synthesis Kit |
BIO-65053/S |
Bioline |
Sample |
Ask for price |
SensiFAST cDNA Synthesis Kit |
BIO-65054 |
Bioline |
250 Reactions |
Ask for price |
The pliability of the technique was demonstrated by cloning a number of copies of an orthobunyavirus L genome phase. This necessary step in reverse genetics expertise improvement opens novel avenues for the evaluation of virus variability mixed with phenotypical characterization of recombinant viruses at a clonal stage.