Comparative evaluation of cDNA library construction approaches for RNA-Seq analysis from low RNA-content human specimens.

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Comparative evaluation of cDNA library construction approaches for RNA-Seq analysis from low RNA-content human specimens.

With the emergence of RNA sequencing applied sciences, metatranscriptomic research are quickly gaining consideration as they concurrently present perception into gene expression profiles and subsequently illness affiliation pathways of microbial pathogens and their hosts. This strategy, subsequently, holds promise for applicability in infectious illness diagnostics.

A problem of this strategy within the scientific setting is the low quantity and high quality of RNA, particularly microbial RNA in most clinically-infected specimens. Right here, we in contrast two commercially accessible stranded cDNA library preparation kits, the NuGEN Ovation SoLo RNA-Seq System and the Illumina TruSeq Stranded Complete RNA, utilizing RNA extracted from synovial and sonicate fluids from a topic with periprosthetic joint an infection. The Ovation SoLo RNA-Seq System supplied extra helpful transcriptomic information for the infecting bacterium, whereas the TruSeq Stranded Complete RNA equipment supplied extra helpful human transcriptomic information.

PacBio full-length cDNA sequencing built-in with RNA-seq reads drastically improves the invention of splicing transcripts in rice.

In eukaryotes, various splicing (AS) vastly expands the variety of transcripts. Nevertheless, it’s difficult to precisely decide full-length splicing isoforms. Just lately, extra research have taken benefit of Pacific Bioscience (PacBio) long-read sequencing to establish full-length transcripts. Nonetheless, the excessive error fee of PacBio reads significantly offsets some great benefits of lengthy reads, particularly for precisely figuring out splicing junctions. To finest capitalize on the options of lengthy reads, we used Illumina RNA-seq reads to enhance PacBio round consensus sequence (CCS) high quality and to validate splicing patterns within the rice transcriptome.

We evaluated the influence of CCS accuracy on the quantity and the validation fee of splicing isoforms, and built-in a complete pipeline of splicing transcripts evaluation by Iso-Seq and RNA-seq (STAIR) to establish the full-length multi-exon isoforms in rice seedling transcriptome (Oryza sativa L. ssp. japonica). STAIR found 11 733 full-length multi-exon isoforms, 6599 greater than the SMRT Portal RS_IsoSeq pipeline did.

Of those splicing isoforms recognized, 4453 (37.9%) have been missed in assembled transcripts from RNA-seq reads, and 5204 (44.4%), together with 268 multi-exon lengthy non-coding RNAs (lncRNAs), weren’t reported within the MSU_osa1r7 annotation. Some randomly chosen unreported splicing junctions have been verified by polymerase chain response (PCR) amplification.

Comparative evaluation of cDNA library construction approaches for RNA-Seq analysis from low RNA-content human specimens.

As well as, we investigated various polyadenylation (APA) occasions in transcripts and recognized 829 main polyadenylation [poly(A)] web site clusters (PACs). The evaluation of splicing isoforms and APA occasions will facilitate the annotation of the rice genome and research on the expression and polyadenylation of AS genes in numerous developmental phases or progress situations of rice.

An RNA Binding Peptide Consisting of 4 Varieties of Amino Acid by in Vitro Choice Utilizing cDNA Show.

RNA-protein interactions have a central function within the residing world. On this article, we examined whether or not primitive peptides (30 residues) consisting of 4 kinds of amino acid (Gly, Ala, Asp, and Val) might work together with tRNA as a mannequin of primitive RNAs within the RNA world. By in vitro choice of binding peptides utilizing the cDNA show methodology, a attribute peptide was chosen from a random peptide library and assayed by electrophoretic mobility shift and pull-down assays. Curiously, the chosen peptide certain to a single-stranded area together with a loop construction of an RNA molecule with some sequence specificity.

Comparability of varied RNA extraction strategies, cDNA preparation and isolation of calmodulin gene from a extremely melanized isolate of apple leaf blotch fungus Marssonina coronaria.

Marssonina coronaria causes apple blotch illness leading to extreme untimely defoliation, and is distributed in lots of main apple-growing areas on the planet. Efficient, dependable and high-quality RNA extraction is an indispensable process in any molecular biology examine. No methodology presently exists for RNA extraction from M. coronaria that produces a excessive amount of melanin-free RNA.
Subsequently, we evaluated eight RNA extraction strategies together with handbook and industrial kits, to yield a ample amount of high-quality and melanin-free RNA. Guide strategies used right here resulted in low high quality and black coloured RNA pellets exhibiting the presence of melanin, regardless of all of the modifications employed to authentic procedures. Nevertheless, these strategies when coupled with clear up resulted in melanin-free RNA.
Alternatively, all industrial kits used have been in a position to yield high-quality melanin-free RNA having variable yields. TRIzol™ Reagent + RNA Clear & Concentrator™-5 and Ambion-PureLink® RNA Mini Package have been discovered to be the perfect strategies because the RNA extracted with these strategies from 15 day previous fungal tradition grown on strong medium have been freed from melanin with good yield. RNA extracted by this improved methodology was utilized for RT-PCR, subsequent PCR amplification, and isolation of calmodulin gene sequences from M. coronaria and contaminated apple leaf items.

COL20A1 cDNA Clone
MBS1267755-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

COL20A1 cDNA Clone
MBS1267755-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

COL11A1 cDNA Clone
MBS1273415-001mgPlasmid02mLGlycerolStockEColi 0.01mgPlasmid+0.2mLGlycerol-Stock-(E-Coli)
EUR 2440

COL19A1 cDNA Clone
MBS1269360-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 1230

COL19A1 cDNA Clone
MBS1269360-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 5500

COL24A1 cDNA Clone
MBS1277012-INQUIRE INQUIRE Ask for price

COL21A1 cDNA Clone
MBS1273026-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 520

COL21A1 cDNA Clone
MBS1273026-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 2295

COL9A1 cDNA Clone
MBS1265882-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

COL9A1 cDNA Clone
MBS1265882-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

COL8A2 cDNA Clone
MBS1267076-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 430

COL8A2 cDNA Clone
MBS1267076-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1890

COL8A1 cDNA Clone
MBS1274339-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 435

COL8A1 cDNA Clone
MBS1274339-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1910

COL4A6 cDNA Clone
MBS1274870-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

COL4A6 cDNA Clone
MBS1274870-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

COL1A1 cDNA Clone
MBS1269552-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 1715

COL1A1 cDNA Clone
MBS1269552-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 7675

COL2A1 cDNA Clone
MBS1276796-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

COL2A1 cDNA Clone
MBS1276796-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

COL3A1 cDNA Clone
MBS1272896-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 1220

COL3A1 cDNA Clone
MBS1272896-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 5435

COL9A3 cDNA Clone
MBS1275831-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 650

COL9A3 cDNA Clone
MBS1275831-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 2880

COL6A1 cDNA Clone
MBS1276495-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 1080

COL6A1 cDNA Clone
MBS1276495-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 4825

COL4A3BP cDNA Clone
MBS1275004-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

COL4A3BP cDNA Clone
MBS1275004-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

Active Collagen Type I Alpha 2 (COL1a2)
APA215Ga61 10ug
EUR 324

Recombinant Collagen Type I Alpha 2 (COL1a2)
RPA215Ca01 10ug
EUR 192

Recombinant Collagen Type I Alpha 2 (COL1a2)
4-RPA215Ca01
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  • 10ug
  • 50ug
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  • 1 mg
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Description: Recombinant Dog Collagen Type I Alpha 2 expressed in: E.coli

Recombinant Collagen Type I Alpha 2 (COL1a2)
RPA215Ga01 10ug
EUR 164

Recombinant Collagen Type I Alpha 2 (COL1a2)
RPA215Ga02 10ug
EUR 166

Recombinant Collagen Type I Alpha 2 (COL1a2)
RPA215Hu01 10ug
EUR 164

Recombinant Collagen Type I Alpha 2 (COL1a2)
4-RPA215Hu01
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  • 10ug
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  • 1 mg
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Description: Recombinant Human Collagen Type I Alpha 2 expressed in: E.coli

Recombinant Collagen Type I Alpha 2 (COL1a2)
RPA215Mu01 10ug
EUR 152

Recombinant Collagen Type I Alpha 2 (COL1a2)
4-RPA215Mu01
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Description: Recombinant Mouse Collagen Type I Alpha 2 expressed in: E.coli

Recombinant Collagen Type I Alpha 2 (COL1a2)
RPA215Ra01 10ug
EUR 180

Lenti-ORF clone of COL1A2 (mGFP-tagged)-Human collagen, type I, alpha 2 (COL1A2)
RC208484L4 10 µg Ask for price

Lenti ORF clone of Col16a1 (mGFP-tagged) - Mouse procollagen, type XVI, alpha 1 (cDNA clone MGC:38435 IMAGE:5346676)
MR211522L4 10 µg Ask for price

Lenti ORF clone of Col9a3 (mGFP-tagged) - Mouse procollagen, type IX, alpha 3 (cDNA clone MGC:32160 IMAGE:5003028)
MR205986L4 10 µg Ask for price

Lenti ORF clone of Col9a1 (mGFP-tagged) - Mouse procollagen, type IX, alpha 1 (cDNA clone MGC:47016 IMAGE:3592397)
MR209987L4 10 µg Ask for price

Lenti ORF clone of Col4a6 (mGFP-tagged) - Mouse procollagen, type IV, alpha 6 (cDNA clone MGC:67937 IMAGE:3500535)
MR211656L4 10 µg Ask for price

Lenti ORF clone of Col2a1 (mGFP-tagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379)
MR211978L4 10 µg Ask for price

Lenti ORF clone of Col4a1 (mGFP-tagged) - Mouse procollagen, type IV, alpha 1 (cDNA clone MGC:91373 IMAGE:6852661)
MR212033L4 10 µg Ask for price

Lenti ORF clone of Col13a1 (mGFP-tagged) - Mouse procollagen, type XIII, alpha 1 (cDNA clone MGC:36116 IMAGE:5025449)
MR208934L4 10 µg Ask for price

Lenti ORF clone of Col16a1 (Myc-DDK-tagged) - Mouse procollagen, type XVI, alpha 1 (cDNA clone MGC:38435 IMAGE:5346676)
MR211522L3 10 µg Ask for price

Lenti-ORF clone of COL1A2 (Myc-DDK-tagged)-Human collagen, type I, alpha 2 (COL1A2)
RC208484L3 10 µg Ask for price

Lenti ORF clone of Col9a3 (Myc-DDK-tagged) - Mouse procollagen, type IX, alpha 3 (cDNA clone MGC:32160 IMAGE:5003028)
MR205986L3 10 µg Ask for price

Lenti ORF clone of Col9a1 (Myc-DDK-tagged) - Mouse procollagen, type IX, alpha 1 (cDNA clone MGC:47016 IMAGE:3592397)
MR209987L3 10 µg Ask for price

Lenti ORF clone of Col4a6 (Myc-DDK-tagged) - Mouse procollagen, type IV, alpha 6 (cDNA clone MGC:67937 IMAGE:3500535)
MR211656L3 10 µg Ask for price

Lenti ORF clone of Col2a1 (Myc-DDK-tagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379)
MR211978L3 10 µg Ask for price

Lenti ORF clone of Col4a1 (Myc-DDK-tagged) - Mouse procollagen, type IV, alpha 1 (cDNA clone MGC:91373 IMAGE:6852661)
MR212033L3 10 µg Ask for price

Lenti ORF clone of Col13a1 (Myc-DDK-tagged) - Mouse procollagen, type XIII, alpha 1 (cDNA clone MGC:36116 IMAGE:5025449)
MR208934L3 10 µg Ask for price

ELISA Kit for Collagen Type I Alpha 2 (COL1a2)
SEA215Ca 96Т
EUR 720

Dog Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Ca-10x96wellstestplate 10x96-wells test plate
EUR 5834.88
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Dog Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Ca-1x48wellstestplate 1x48-wells test plate
EUR 585.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Dog Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Ca-1x96wellstestplate 1x96-wells test plate
EUR 785.28
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Dog Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Ca-5x96wellstestplate 5x96-wells test plate
EUR 3173.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Dog Collagen Type I Alpha 2 (COL1a2) ELISA Kit
4-SEA215Ca
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  • 1 plate of 96 wells
  • 5 plates of 96 wells
  • 10 plates of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Dog Collagen Type I Alpha 2 (COL1a2) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.

ELISA Kit for Collagen Type I Alpha 2 (COL1a2)
SEA215Hu 96Т
EUR 630

ELISA Kit for Collagen Type I Alpha 2 (COL1a2)
SEA215Mu 96Т
EUR 648

Rat Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Ra-10x96wellstestplate 10x96-wells test plate
EUR 5552.14
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Collagen Type I Alpha 2 (COL1a2) in serum, plasma, tissue homogenates and other biological fluids.

Rat Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Ra-1x48wellstestplate 1x48-wells test plate
EUR 562.42
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Collagen Type I Alpha 2 (COL1a2) in serum, plasma, tissue homogenates and other biological fluids.

Rat Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Ra-1x96wellstestplate 1x96-wells test plate
EUR 752.02
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Collagen Type I Alpha 2 (COL1a2) in serum, plasma, tissue homogenates and other biological fluids.

Rat Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Ra-5x96wellstestplate 5x96-wells test plate
EUR 3024.07
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Collagen Type I Alpha 2 (COL1a2) in serum, plasma, tissue homogenates and other biological fluids.

Rat Collagen Type I Alpha 2 (COL1a2) ELISA Kit
4-SEA215Ra
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  • 1 plate of 96 wells
  • 5 plates of 96 wells
  • 10 plates of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Collagen Type I Alpha 2 (COL1a2) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Hu-10x96wellstestplate 10x96-wells test plate
EUR 5128.02
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type I Alpha 2 (COL1a2) in serum, plasma, tissue homogenates and other biological fluids.

Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Hu-1x48wellstestplate 1x48-wells test plate
EUR 527.48
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type I Alpha 2 (COL1a2) in serum, plasma, tissue homogenates and other biological fluids.

Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Hu-1x96wellstestplate 1x96-wells test plate
EUR 702.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type I Alpha 2 (COL1a2) in serum, plasma, tissue homogenates and other biological fluids.

Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Hu-5x96wellstestplate 5x96-wells test plate
EUR 2799.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type I Alpha 2 (COL1a2) in serum, plasma, tissue homogenates and other biological fluids.

Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit
4-SEA215Hu
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  • 1 plate of 96 wells
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Collagen Type I Alpha 2 (COL1a2) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Mu-10x96wellstestplate 10x96-wells test plate
EUR 5269.39
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Mu-1x48wellstestplate 1x48-wells test plate
EUR 539.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Mu-1x96wellstestplate 1x96-wells test plate
EUR 718.75
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Mu-5x96wellstestplate 5x96-wells test plate
EUR 2874.38
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit
4-SEA215Mu
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  • 1 plate of 96 wells
  • 5 plates of 96 wells
  • 10 plates of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Collagen Type I Alpha 2 (COL1a2) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Cattle Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Bo-10x96wellstestplate 10x96-wells test plate
EUR 6117.62
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Cattle Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Bo-1x48wellstestplate 1x48-wells test plate
EUR 608.98
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Cattle Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Bo-1x96wellstestplate 1x96-wells test plate
EUR 818.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Cattle Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Bo-5x96wellstestplate 5x96-wells test plate
EUR 3323.45
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Cattle Collagen Type I Alpha 2 (COL1a2) ELISA Kit
4-SEA215Bo
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  • 1 plate of 96 wells
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  • 10 plates of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Collagen Type I Alpha 2 (COL1a2) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Rb-10x96wellstestplate 10x96-wells test plate
EUR 5552.14
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rabbit Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Rb-1x48wellstestplate 1x48-wells test plate
EUR 562.42
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rabbit Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Rb-1x96wellstestplate 1x96-wells test plate
EUR 752.02
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rabbit Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit
SEA215Rb-5x96wellstestplate 5x96-wells test plate
EUR 3024.07
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rabbit Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit
4-SEA215Rb
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  • 1 plate of 96 wells
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rabbit Collagen Type I Alpha 2 (COL1a2) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.

Rabbit Anti Human COL1A2 Monoclonal Clone AFD-3
IRBAHUCOL1A2AFD3C100UL each
EUR 496
Description: Rabbit Anti Human COL1A2 Monoclonal Clone AFD-3

Col16a1 (GFP-tagged) - Mouse procollagen, type XVI, alpha 1 (cDNA clone MGC:38435 IMAGE:5346676)
MG211522 10 µg Ask for price

Monoclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
MAA215Hu22 100ul
EUR 235

Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
PAA215Ca01 100ul
EUR 252

Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
PAA215Ga01 100ul
EUR 239

Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
PAA215Hu01 100ul
EUR 221

Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
PAA215Mu01 100ul
EUR 227

Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
PAA215Rb51 100ul
EUR 239

Col4a6 (GFP-tagged) - Mouse procollagen, type IV, alpha 6 (cDNA clone MGC:67937 IMAGE:3500535)
MG211656 10 µg Ask for price

Col2a1 (GFP-tagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379)
MG211978 10 µg Ask for price

Col2a1 (GFP-tagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:90638 IMAGE:30537724)
MG212008 10 µg Ask for price

Col4a1 (GFP-tagged) - Mouse procollagen, type IV, alpha 1 (cDNA clone MGC:91373 IMAGE:6852661)
MG212033 10 µg Ask for price

Col4a5 (GFP-tagged) - Mouse procollagen, type IV, alpha 5 (cDNA clone MGC:91198 IMAGE:5343488)
MG212066 10 µg Ask for price

Col9a1 (GFP-tagged) - Mouse procollagen, type IX, alpha 1 (cDNA clone MGC:47016 IMAGE:3592397)
MG209987 10 µg Ask for price

Col9a3 (GFP-tagged) - Mouse procollagen, type IX, alpha 3 (cDNA clone MGC:32160 IMAGE:5003028)
MG205986 10 µg Ask for price

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Dog)
4-PAA215Ca01
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  • 20ul
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  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Dog Collagen Type I Alpha 2 (COL1a2)

Col13a1 (GFP-tagged) - Mouse procollagen, type XIII, alpha 1 (cDNA clone MGC:36116 IMAGE:5025449)
MG208934 10 µg Ask for price

Col13a1 (untagged) - Mouse procollagen, type XIII, alpha 1 (cDNA clone MGC:36116 IMAGE:5025449), (10ug)
MC218153 10 µg Ask for price

Col16a1 (untagged) - Mouse procollagen, type XVI, alpha 1 (cDNA clone MGC:38435 IMAGE:5346676), (10ug)
MC223116 10 µg Ask for price

Col9a3 (untagged) - Mouse procollagen, type IX, alpha 3 (cDNA clone MGC:32160 IMAGE:5003028), (10ug)
MC217694 10 µg Ask for price

Col2a1 (untagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379), (10ug)
MC224565 10 µg Ask for price

Col4a1 (untagged) - Mouse procollagen, type IV, alpha 1 (cDNA clone MGC:91373 IMAGE:6852661), (10ug)
MC224566 10 µg Ask for price

Col4a6 (untagged) - Mouse procollagen, type IV, alpha 6 (cDNA clone MGC:67937 IMAGE:3500535), (10ug)
MC223028 10 µg Ask for price

Col9a1 (untagged) - Mouse procollagen, type IX, alpha 1 (cDNA clone MGC:47016 IMAGE:3592397), (10ug)
MC220709 10 µg Ask for price

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Mouse)
4-PAA215Mu01
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Description: A Rabbit polyclonal antibody against Mouse Collagen Type I Alpha 2 (COL1a2)

COL25A1 (untagged)-Human collagen, type XXV, alpha 1 (cDNA clone MGC:33431 IMAGE:5268875), complete cds
SC122503 10 µg Ask for price

Col13a1 (Myc-DDK-tagged) - Mouse procollagen, type XIII, alpha 1 (cDNA clone MGC:36116 IMAGE:5025449)
MR208934 10 µg Ask for price

Col16a1 (Myc-DDK-tagged) - Mouse procollagen, type XVI, alpha 1 (cDNA clone MGC:38435 IMAGE:5346676)
MR211522 10 µg Ask for price

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Dog), PE
4-PAA215Ca01-PE
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  • 20ul
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  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Dog Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with PE.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Dog), APC
4-PAA215Ca01-APC
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  • 20ul
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Description: A Rabbit polyclonal antibody against Dog Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with APC.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Dog), Cy3
4-PAA215Ca01-Cy3
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  • 20ul
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  • 1ml
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Description: A Rabbit polyclonal antibody against Dog Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with Cy3.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Dog), HRP
4-PAA215Ca01-HRP
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  • 20ul
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  • 1ml
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Description: A Rabbit polyclonal antibody against Dog Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with HRP.

Col9a3 (Myc-DDK-tagged) - Mouse procollagen, type IX, alpha 3 (cDNA clone MGC:32160 IMAGE:5003028)
MR205986 10 µg Ask for price

Col9a1 (Myc-DDK-tagged) - Mouse procollagen, type IX, alpha 1 (cDNA clone MGC:47016 IMAGE:3592397)
MR209987 10 µg Ask for price

Col4a6 (Myc-DDK-tagged) - Mouse procollagen, type IV, alpha 6 (cDNA clone MGC:67937 IMAGE:3500535)
MR211656 10 µg Ask for price

Col2a1 (Myc-DDK-tagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379)
MR211978 10 µg Ask for price

Col4a1 (Myc-DDK-tagged) - Mouse procollagen, type IV, alpha 1 (cDNA clone MGC:91373 IMAGE:6852661)
MR212033 10 µg Ask for price

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Dog), FITC
4-PAA215Ca01-FITC
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Dog Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with FITC.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Mouse), PE
4-PAA215Mu01-PE
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  • 20ul
  • 100ul
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  • 1ml
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Description: A Rabbit polyclonal antibody against Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with PE.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Mouse), APC
4-PAA215Mu01-APC
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  • 20ul
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  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with APC.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Mouse), Cy3
4-PAA215Mu01-Cy3
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with Cy3.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Mouse), HRP
4-PAA215Mu01-HRP
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with HRP.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Mouse), FITC
4-PAA215Mu01-FITC
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  • 20ul
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  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with FITC.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Human, Mouse)
4-PAA215Hu01
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Human, Mouse Collagen Type I Alpha 2 (COL1a2)

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Dog), Biotinylated
4-PAA215Ca01-Biotin
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Dog Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with Biotin.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Mouse), Biotinylated
4-PAA215Mu01-Biotin
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with Biotin.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Dog), APC-Cy7
4-PAA215Ca01-APC-Cy7
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Dog Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with APC-Cy7.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Human, Mouse), PE
4-PAA215Hu01-PE
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Human, Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with PE.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Human, Mouse), APC
4-PAA215Hu01-APC
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Human, Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with APC.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Human, Mouse), Cy3
4-PAA215Hu01-Cy3
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Human, Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with Cy3.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Human, Mouse), HRP
4-PAA215Hu01-HRP
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  • 20ul
  • 100ul
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  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Human, Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with HRP.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Mouse), APC-Cy7
4-PAA215Mu01-APC-Cy7
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with APC-Cy7.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Human, Mouse), FITC
4-PAA215Hu01-FITC
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  • 20ul
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Description: A Rabbit polyclonal antibody against Human, Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with FITC.

COLEC11 cDNA Clone
MBS1277053-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

COLEC11 cDNA Clone
MBS1277053-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

COLEC10 cDNA Clone
MBS1272322-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

COLEC10 cDNA Clone
MBS1272322-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

COLEC12 cDNA Clone
MBS1271673-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 700

COLEC12 cDNA Clone
MBS1271673-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 3110

Lenti ORF clone of Col4a3bp (mGFP-tagged) - Mouse procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein (cDNA clone MGC:27595 IMAGE
MR206816L4 10 µg Ask for price

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Human, Mouse), Biotinylated
4-PAA215Hu01-Biotin
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
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Description: A Rabbit polyclonal antibody against Human, Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with Biotin.

Lenti ORF clone of Col4a3bp (Myc-DDK-tagged) - Mouse procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein (cDNA clone MGC:27595 IM
MR206816L3 10 µg Ask for price

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Human, Mouse), APC-Cy7
4-PAA215Hu01-APC-Cy7
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  • 20ul
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  • 1ml
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Description: A Rabbit polyclonal antibody against Human, Mouse Collagen Type I Alpha 2 (COL1a2). This antibody is labeled with APC-Cy7.

COL1A2 cloning plasmid
CSB-CL005728HU-10ug 10ug
EUR 1934.4
Description: A cloning plasmid for the COL1A2 gene.

Lenti ORF particles, Col13a1 (GFP-tagged) - Mouse procollagen, type XIII, alpha 1 (cDNA clone MGC:36116 IMAGE:5025449), 200ul, >10^7 TU/mL
MR208934L4V 200 µl Ask for price

Lenti ORF particles, Col16a1 (GFP-tagged) - Mouse procollagen, type XVI, alpha 1 (cDNA clone MGC:38435 IMAGE:5346676), 200ul, >10^7 TU/mL
MR211522L4V 200 µl Ask for price

Lenti ORF particles, Col9a3 (GFP-tagged) - Mouse procollagen, type IX, alpha 3 (cDNA clone MGC:32160 IMAGE:5003028), 200ul, >10^7 TU/mL
MR205986L4V 200 µl Ask for price

Lenti ORF particles, Col9a1 (GFP-tagged) - Mouse procollagen, type IX, alpha 1 (cDNA clone MGC:47016 IMAGE:3592397), 200ul, >10^7 TU/mL
MR209987L4V 200 µl Ask for price

Lenti ORF particles, Col4a6 (GFP-tagged) - Mouse procollagen, type IV, alpha 6 (cDNA clone MGC:67937 IMAGE:3500535), 200ul, >10^7 TU/mL
MR211656L4V 200 µl Ask for price
These strategies are extra time efficient than conventional strategies and take solely an hour to finish. To our information, that is the primary report on the strategy of isolation of high-quality RNA for cDNA synthesis in addition to isolation of the calmodulin gene sequence from this fungus.
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