Comparative evaluation of cDNA library construction approaches for RNA-Seq analysis from low RNA-content human specimens.

Comparative evaluation of cDNA library construction approaches for RNA-Seq analysis from low RNA-content human specimens.

With the emergence of RNA sequencing applied sciences, metatranscriptomic research are quickly gaining consideration as they concurrently present perception into gene expression profiles and subsequently illness affiliation pathways of microbial pathogens and their hosts. This strategy, subsequently, holds promise for applicability in infectious illness diagnostics.

A problem of this strategy within the scientific setting is the low quantity and high quality of RNA, particularly microbial RNA in most clinically-infected specimens. Right here, we in contrast two commercially accessible stranded cDNA library preparation kits, the NuGEN Ovation SoLo RNA-Seq System and the Illumina TruSeq Stranded Complete RNA, utilizing RNA extracted from synovial and sonicate fluids from a topic with periprosthetic joint an infection. The Ovation SoLo RNA-Seq System supplied extra helpful transcriptomic information for the infecting bacterium, whereas the TruSeq Stranded Complete RNA equipment supplied extra helpful human transcriptomic information.

PacBio full-length cDNA sequencing built-in with RNA-seq reads drastically improves the invention of splicing transcripts in rice.

In eukaryotes, various splicing (AS) vastly expands the variety of transcripts. Nevertheless, it’s difficult to precisely decide full-length splicing isoforms. Just lately, extra research have taken benefit of Pacific Bioscience (PacBio) long-read sequencing to establish full-length transcripts. Nonetheless, the excessive error fee of PacBio reads significantly offsets some great benefits of lengthy reads, particularly for precisely figuring out splicing junctions. To finest capitalize on the options of lengthy reads, we used Illumina RNA-seq reads to enhance PacBio round consensus sequence (CCS) high quality and to validate splicing patterns within the rice transcriptome.

We evaluated the influence of CCS accuracy on the quantity and the validation fee of splicing isoforms, and built-in a complete pipeline of splicing transcripts evaluation by Iso-Seq and RNA-seq (STAIR) to establish the full-length multi-exon isoforms in rice seedling transcriptome (Oryza sativa L. ssp. japonica). STAIR found 11 733 full-length multi-exon isoforms, 6599 greater than the SMRT Portal RS_IsoSeq pipeline did.

Of those splicing isoforms recognized, 4453 (37.9%) have been missed in assembled transcripts from RNA-seq reads, and 5204 (44.4%), together with 268 multi-exon lengthy non-coding RNAs (lncRNAs), weren’t reported within the MSU_osa1r7 annotation. Some randomly chosen unreported splicing junctions have been verified by polymerase chain response (PCR) amplification.

Comparative evaluation of cDNA library construction approaches for RNA-Seq analysis from low RNA-content human specimens.

As well as, we investigated various polyadenylation (APA) occasions in transcripts and recognized 829 main polyadenylation [poly(A)] web site clusters (PACs). The evaluation of splicing isoforms and APA occasions will facilitate the annotation of the rice genome and research on the expression and polyadenylation of AS genes in numerous developmental phases or progress situations of rice.

An RNA Binding Peptide Consisting of 4 Varieties of Amino Acid by in Vitro Choice Utilizing cDNA Show.

RNA-protein interactions have a central function within the residing world. On this article, we examined whether or not primitive peptides (30 residues) consisting of 4 kinds of amino acid (Gly, Ala, Asp, and Val) might work together with tRNA as a mannequin of primitive RNAs within the RNA world. By in vitro choice of binding peptides utilizing the cDNA show methodology, a attribute peptide was chosen from a random peptide library and assayed by electrophoretic mobility shift and pull-down assays. Curiously, the chosen peptide certain to a single-stranded area together with a loop construction of an RNA molecule with some sequence specificity.

Comparability of varied RNA extraction strategies, cDNA preparation and isolation of calmodulin gene from a extremely melanized isolate of apple leaf blotch fungus Marssonina coronaria.

Marssonina coronaria causes apple blotch illness leading to extreme untimely defoliation, and is distributed in lots of main apple-growing areas on the planet. Efficient, dependable and high-quality RNA extraction is an indispensable process in any molecular biology examine. No methodology presently exists for RNA extraction from M. coronaria that produces a excessive amount of melanin-free RNA.
Subsequently, we evaluated eight RNA extraction strategies together with handbook and industrial kits, to yield a ample amount of high-quality and melanin-free RNA. Guide strategies used right here resulted in low high quality and black coloured RNA pellets exhibiting the presence of melanin, regardless of all of the modifications employed to authentic procedures. Nevertheless, these strategies when coupled with clear up resulted in melanin-free RNA.
Alternatively, all industrial kits used have been in a position to yield high-quality melanin-free RNA having variable yields. TRIzol™ Reagent + RNA Clear & Concentrator™-5 and Ambion-PureLink® RNA Mini Package have been discovered to be the perfect strategies because the RNA extracted with these strategies from 15 day previous fungal tradition grown on strong medium have been freed from melanin with good yield. RNA extracted by this improved methodology was utilized for RT-PCR, subsequent PCR amplification, and isolation of calmodulin gene sequences from M. coronaria and contaminated apple leaf items.

Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit

DLR-COL1a2-Mu-48T 48T
EUR 586.8
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Collagen Type I Alpha 2 (COL1a2) in samples from serum, plasma or other biological fluids.

Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit

DLR-COL1a2-Mu-96T 96T
EUR 762
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Collagen Type I Alpha 2 (COL1a2) in samples from serum, plasma or other biological fluids.

Rat Collagen Type I Alpha 2 (COL1a2) ELISA Kit

DLR-COL1a2-Ra-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Collagen Type I Alpha 2 (COL1a2) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Collagen Type I Alpha 2 (COL1a2) ELISA Kit

DLR-COL1a2-Ra-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Rat Collagen Type I Alpha 2 (COL1a2) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit

DLR-COL1a2-Rb-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Collagen Type I Alpha 2 (COL1a2) in samples from serum, plasma or other biological fluids.

Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit

DLR-COL1a2-Rb-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Collagen Type I Alpha 2 (COL1a2) in samples from serum, plasma or other biological fluids.

Bovine Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-b-48Tests 48 Tests
EUR 666

Bovine Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-b-96Tests 96 Tests
EUR 925.2

Canine Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-c-48Tests 48 Tests
EUR 639.6

Canine Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-c-96Tests 96 Tests
EUR 888

Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-Hu-48Tests 48 Tests
EUR 573.6

Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-Hu-96Tests 96 Tests
EUR 794.4

Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-Mu-48Tests 48 Tests
EUR 586.8

Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-Mu-96Tests 96 Tests
EUR 812.4

Rat Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-Ra-48Tests 48 Tests
EUR 613.2

Rat Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-Ra-96Tests 96 Tests
EUR 850.8

Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-Rb-48Tests 48 Tests
EUR 613.2

Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RD-COL1a2-Rb-96Tests 96 Tests
EUR 850.8

Bovine Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-b-48Tests 48 Tests
EUR 696

Bovine Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-b-96Tests 96 Tests
EUR 968.4

Canine Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-c-48Tests 48 Tests
EUR 668.4

Canine Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-c-96Tests 96 Tests
EUR 928.8

Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-Hu-48Tests 48 Tests
EUR 600

Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-Hu-96Tests 96 Tests
EUR 830.4

Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-Mu-48Tests 48 Tests
EUR 613.2

Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-Mu-96Tests 96 Tests
EUR 850.8

Rat Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-Ra-48Tests 48 Tests
EUR 640.8

Rat Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-Ra-96Tests 96 Tests
EUR 890.4

Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-Rb-48Tests 48 Tests
EUR 640.8

Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit

RDR-COL1a2-Rb-96Tests 96 Tests
EUR 890.4

Genorise® Chicken TNFa cDNA Clone

GR147001 - Ask for price

Genorise® Chicken IFNg cDNA Clone

GR147002 10 µg
EUR 183.6

Genorise® Chicken IL-6 cDNA Clone

GR147003 10 µg
EUR 183.6

Genorise® Chicken IL-4 cDNA Clone

GR147004 10 µg
EUR 183.6

Genorise® Chicken IL-10 cDNA Clone

GR147005 10 µg
EUR 183.6

Col1a2/ Rat Col1a2 ELISA Kit

ELI-33673r 96 Tests
EUR 1063.2

COL1a2/ Rat COL1a2 ELISA Kit

ELA-E2570r 96 Tests
EUR 1063.2

Anti-ASFV P72 Antibody | MASF-5A-Clone #10E11

MASF-5A-Clone-10E11 1 mg
EUR 667
Description:

Anti-ASFV P72 Antibody | MASF-5A-Clone #10E11 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-ASFV P72 Antibody | MASF-5A-Clone #10G5

MASF-5A-Clone-10G5 1 mg
EUR 667
Description:

Anti-ASFV P72 Antibody | MASF-5A-Clone #10G5 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Genorise® Human GLUT1 cDNA Clone, 10 ug

GR154002 10 µg
EUR 183.6

Genorise® Human cTnT cDNA Clone, 10 ug

GR154003 10 µg
EUR 183.6

Genorise® Human TNN3 cDNA Clone, 10 ug

GR154004 10 µg
EUR 183.6

Genorise® Human BNP cDNA Clone, 10 ug

GR154005 10 µg
EUR 183.6

Genorise® Human Myoglobin cDNA Clone, 10 ug

GR154006 10 µg
EUR 183.6

Genorise® Human ANP cDNA Clone, 10 ug

GR154008 10 µg
EUR 183.6

Genorise® Human Myeloperoxidase cDNA Clone, 10 ug

GR154009 10 µg
EUR 183.6

Genorise® Human GPBB cDNA Clone, 10 ug

GR154010 10 µg
EUR 183.6

Genorise® Human CRP cDNA Clone, 10 ug

GR154011 10 µg
EUR 183.6

Genorise® Human MyBPC3 cDNA Clone, 10 ug

GR154012 10 µg
EUR 183.6

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#3B11

MALV-30A-Clone-3B11 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#3B11 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#5A4

MALV-30A-Clone-5A4 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#5A4 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6C2

MALV-30A-Clone-6C2 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6C2 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6D7

MALV-30A-Clone-6D7 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6D7 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

COL1A2 antibody

70R-49569 100 ul
EUR 292.8
Description: Purified Polyclonal COL1A2 antibody

COL1A2 antibody

70R-49570 100 ul
EUR 544.8
Description: Purified Polyclonal COL1A2 antibody

COL1A2 antibody

70R-16497 50 ul
EUR 522
Description: Rabbit polyclonal COL1A2 antibody

COL1A2 antibody

70R-31222 100 ug
EUR 392.4
Description: Rabbit polyclonal COL1A2 antibody

COL1A2 Antibody

35634-100ul 100ul
EUR 302.4

COL1A2 siRNA

20-abx901176
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

COL1A2 siRNA

20-abx912408
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

COL1A2 siRNA

20-abx912409
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

COL1A2 Antibody

1-CSB-PA001742
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against COL1A2. Recognizes COL1A2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/10000

COL1A2 Antibody

1-CSB-PA001743
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against COL1A2. Recognizes COL1A2 from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000

COL1A2 Antibody

CSB-PA571805-
EUR 402
Description: A polyclonal antibody against COL1A2. Recognizes COL1A2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

COL1A2 Antibody

CSB-PA571805-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against COL1A2. Recognizes COL1A2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

COL1A2 Antibody

1-CSB-PA182143
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against COL1A2. Recognizes COL1A2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:5000, IHC:1:100-1:300

COL1A2 Antibody

1-CSB-PA201854
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against COL1A2. Recognizes COL1A2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

COL1A2 Antibody

1-CSB-PA225534
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against COL1A2. Recognizes COL1A2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-2000, ELISA:1:10000-20000

COL1A2 Antibody

1-CSB-PA005728GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against COL1A2. Recognizes COL1A2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

Genorise® Sheep IL-6 cDNA Clone, 10 ug

GR151001 10 µg
EUR 183.6

Genorise® Ovine IL-2 cDNA Clone, 10 ug

GR151002 10 µg
EUR 183.6

Genorise® Ovine IL-4 cDNA Clone, 10 ug

GR151003 10 µg
EUR 183.6

Genorise® Bovine IL-1b cDNA Clone, 10 ug

GR152001 10 µg
EUR 183.6

Genorise® Rabbit IL-1b cDNA Clone, 10 ug

GR153001 10 µg
EUR 183.6

Genorise® Human H-FABP cDNA Clone, 10 ug

GR154001 10 µg
EUR 183.6

Genorise® Human Lp-PLA2 cDNA Clone, 10 ug

GR154007 10 µg
EUR 183.6

Genorise® Human PAPP-A cDNA Clone, 10 ug

GR154013 10 µg
EUR 183.6

Genorise® Human NT-proBNP cDNA Clone, 10 ug

GR154014 10 µg
EUR 183.6

Genorise® Human Pro-C3 cDNA Clone, 10 ug

GR154015 10 µg
EUR 183.6

Genorise® Canine NGF-B cDNA Clone, 10 ug

GR159001 10 µg
EUR 183.6

Genorise® Canine IL-7 cDNA Clone, 10 ug

GR159002 10 µg
EUR 183.6

Anti-COL1A2 Antibody

A00624-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for COL1A2 Antibody (COL1A2) detection.tested for WB in Human, Mouse, Rat.

COL1A2 Polyclonal Antibody

40771-100ul 100ul
EUR 302.4

COL1A2 Polyclonal Antibody

40771-50ul 50ul
EUR 224.4

COL1A2 Blocking Peptide

20-abx061969
  • EUR 309.60
  • EUR 460.80
  • 1 mg
  • 5 mg

COL1A2 Blocking Peptide

20-abx062444
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

COL1A2 Blocking Peptide

20-abx062445
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

COL1A2 Polyclonal Antibody

ABP51021-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of COL1A2 from Human, Mouse, Rat. This COL1A2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human COL1A2 at AA range: 1-80

COL1A2 Polyclonal Antibody

ABP51021-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of COL1A2 from Human, Mouse, Rat. This COL1A2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human COL1A2 at AA range: 1-80

COL1A2 Polyclonal Antibody

ABP51021-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of COL1A2 from Human, Mouse, Rat. This COL1A2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human COL1A2 at AA range: 1-80

COL1A2 Polyclonal Antibody

ABP51022-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of COL1A2 from Human. This COL1A2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human COL1A2 at AA range: 440-520

COL1A2 Polyclonal Antibody

ABP51022-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of COL1A2 from Human. This COL1A2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human COL1A2 at AA range: 440-520

COL1A2 Polyclonal Antibody

ABP51022-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of COL1A2 from Human. This COL1A2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human COL1A2 at AA range: 440-520

COL1A2 Rabbit pAb

A16699-100ul 100 ul
EUR 369.6

COL1A2 Rabbit pAb

A16699-200ul 200 ul
EUR 550.8

COL1A2 Rabbit pAb

A16699-20ul 20 ul
EUR 219.6

COL1A2 Rabbit pAb

A16699-50ul 50 ul
EUR 267.6

COL1A2 Rabbit pAb

A5786-100ul 100 ul
EUR 369.6

COL1A2 Rabbit pAb

A5786-200ul 200 ul
EUR 550.8

COL1A2 Rabbit pAb

A5786-20ul 20 ul
EUR 219.6

COL1A2 Rabbit pAb

A5786-50ul 50 ul
EUR 267.6

COL1A2 / COL1A1 Antibody

20-abx329798
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

COL1A2 Conjugated Antibody

C35634 100ul
EUR 476.4

COL1A2 cloning plasmid

CSB-CL005728HU-10ug 10ug
EUR 1934.4
Description: A cloning plasmid for the COL1A2 gene.

COL1A2/COL1A1 Antibody

1-CSB-PA164287
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against COL1A2/COL1A1. Recognizes COL1A2/COL1A1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-2000, ELISA:1:10000-20000

COL1A2 Polyclonal Antibody

ES2020-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against COL1A2 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

COL1A2 Polyclonal Antibody

ES2020-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against COL1A2 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

COL1A2 Polyclonal Antibody

ES2021-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against COL1A2 from Human. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

COL1A2 Polyclonal Antibody

ES2021-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against COL1A2 from Human. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Anti-COL1A2 antibody

STJ119121 100 µl
EUR 332.4

Anti-COL1A2 antibody

STJ92383 200 µl
EUR 236.4
Description: COL1A2 is a protein encoded by the COL1A2 gene which is approximately 129,3 kDa. COL1A2 is secreted into the extracellular space. It is involved in collagen chain trimerization, the integrin pathway, ERK signalling and focal adhesion. It is a pro-alpha-2 chain of type I collagen whose triple helix comprises two alpha-1 chains and one alpha-2 chain. COL1A2 is expressed in tendons, ligaments and bones. Mutations in the COL1A2 gene may result in Ehlers-Danlos syndrome and osteogenesis imperfecta. STJ92383 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of COL1A2 protein.

Anti-COL1A2 antibody

STJ92384 200 µl
EUR 236.4
Description: Rabbit polyclonal to COL1A2.

Anti-COL1A2 antibody

STJ28351 100 µl
EUR 332.4
Description: This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Anti-COL1A2 (7E11)

YF-MA12496 100 ug
EUR 435.6
Description: Mouse monoclonal to COL1A2

Recombinant Collagen Type I Alpha 2 (COL1a2)

4-RPA215Ca01
  • EUR 636.10
  • EUR 294.00
  • EUR 2055.36
  • EUR 765.12
  • EUR 1410.24
  • EUR 501.60
  • EUR 4958.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Dog Collagen Type I Alpha 2 expressed in: E.coli

Recombinant Collagen Type I Alpha 2 (COL1a2)

4-RPA215Hu01
  • EUR 560.83
  • EUR 273.60
  • EUR 1773.12
  • EUR 671.04
  • EUR 1222.08
  • EUR 451.20
  • EUR 4252.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Collagen Type I Alpha 2 expressed in: E.coli

Recombinant Collagen Type I Alpha 2 (COL1a2)

4-RPA215Mu01
  • EUR 528.58
  • EUR 265.20
  • EUR 1652.16
  • EUR 630.72
  • EUR 1141.44
  • EUR 429.60
  • EUR 3950.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Collagen Type I Alpha 2 expressed in: E.coli

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 678.00
  • EUR 577.20
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Novo? cDNA Kit

M1165-100
EUR 424.8

Novo? cDNA Kit

M1165-25
EUR 320.4

Evo? cDNA Supermix

M1168-100
EUR 457.2

Evo? cDNA Supermix

M1168-25
EUR 320.4

Novo? cDNA Supermix

M1169-100
EUR 529.2

Novo? cDNA Supermix

M1169-25
EUR 346.8

COL1A2 (Cleaved-Gly1102) Antibody

20-abx015578
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Human COL1A2 shRNA Plasmid

20-abx950894
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Cleaved-COL1A2 (G1102) Antibody

1-CSB-PA000039
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Cleaved-COL1A2 (G1102). Recognizes Cleaved-COL1A2 (G1102) from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000

Mouse COL1A2 shRNA Plasmid

20-abx969768
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Rat COL1A2 shRNA Plasmid

20-abx986911
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Porcine COL1a2 ELISA Kit

EPC0329 96Tests
EUR 625.2

Rabbit COL1a2 ELISA Kit

ERTC0329 96Tests
EUR 625.2

Rat COL1a2 ELISA Kit

ERC0329 96Tests
EUR 625.2

Mouse COL1a2 ELISA Kit

EMC0329 96Tests
EUR 625.2

Monkey COL1a2 ELISA Kit

EMKC0329 96Tests
EUR 625.2

Canine COL1A2 ELISA KIT

ELI-51021d 96 Tests
EUR 1113.6

Human COL1a2 ELISA Kit

EHC0329 96Tests
EUR 625.2

Goat COL1a2 ELISA Kit

EGTC0329 96Tests
EUR 625.2

Canine COL1a2 ELISA Kit

ECC0329 96Tests
EUR 625.2

Chicken COL1a2 ELISA Kit

ECKC0329 96Tests
EUR 625.2

Anserini COL1a2 ELISA Kit

EAC0329 96Tests
EUR 625.2

Bovine COL1a2 ELISA Kit

EBC0329 96Tests
EUR 625.2

Sheep COL1a2 ELISA Kit

ESC0329 96Tests
EUR 625.2

Canine COL1a2 ELISA Kit

ELA-E2570d 96 Tests
EUR 1113.6

Collagen I Antibody / COL1A2

F41539-0.08ML 0.08 ml
EUR 165

Collagen I Antibody / COL1A2

F41539-0.4ML 0.4 ml
EUR 379

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Dog)

4-PAA215Ca01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Dog Collagen Type I Alpha 2 (COL1a2)

Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody (Mouse)

4-PAA215Mu01
  • EUR 283.20
  • EUR 2805.60
  • EUR 703.20
  • EUR 352.80
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Collagen Type I Alpha 2 (COL1a2)

Cattle Collagen Type I Alpha 2 (COL1a2) ELISA Kit

SEA215Bo-10x96wellstestplate 10x96-wells test plate
EUR 6117.62
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Cattle Collagen Type I Alpha 2 (COL1a2) ELISA Kit

SEA215Bo-1x48wellstestplate 1x48-wells test plate
EUR 608.98
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.

Cattle Collagen Type I Alpha 2 (COL1a2) ELISA Kit

SEA215Bo-1x96wellstestplate 1x96-wells test plate
EUR 818.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Collagen Type I Alpha 2 (COL1a2) in serum, plasma and other biological fluids.
These strategies are extra time efficient than conventional strategies and take solely an hour to finish. To our information, that is the primary report on the strategy of isolation of high-quality RNA for cDNA synthesis in addition to isolation of the calmodulin gene sequence from this fungus.

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